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Conference Paper: Human cathepsinG-cre mice for myeloid-specific gene targeting

TitleHuman cathepsinG-cre mice for myeloid-specific gene targeting
Authors
Issue Date2001
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DGD
Citation
The 14th International Congress of Developmental Biology, Kyoto, Japan, 8-12 July 2001, In Development, Growth and Differentiation, 2001, v. 43 suppl. 1, p. S131, abstract no. S13-P4 How to Cite?
AbstractThe flexibility and success of conditional gene targeting depends on the availability of tissue or cell lineage specific expression of cre recombinase in mouse mutants. The human cathepsin G (hCG) gene is expressed exclusively in promyelocytes/ promonocytes and encodes a neutral serine protease that is package in the azurophil granules of myeloid cells. With the aim of studying human leukaemia using mutant mouse models, we attempted to generate an early myeloid cell-specific cre recombinase transgenic mouse line, hCG-cre, using the hCG promoter. A DNA construct with the cre gene inserted upstream of the coding region of a hCG genomic fragment was used for transgenic mouse production. To assess the tissue specificity and efficiency of the cre recombinase in the hCG-cre mouse line, we would cross the hCG-cre mice with the Z/AP reporter mouse line. The activity and specificity of the alkaline phosphatase reporter in the bone marrow and peripheral blood of the double mutant mice will be examined.
Persistent Identifierhttp://hdl.handle.net/10722/96454
ISSN
2015 Impact Factor: 2.412
2015 SCImago Journal Rankings: 1.561

 

DC FieldValueLanguage
dc.contributor.authorKong, CTen_HK
dc.contributor.authorSo, CWen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorSham, MHen_HK
dc.date.accessioned2010-09-25T16:34:18Z-
dc.date.available2010-09-25T16:34:18Z-
dc.date.issued2001en_HK
dc.identifier.citationThe 14th International Congress of Developmental Biology, Kyoto, Japan, 8-12 July 2001, In Development, Growth and Differentiation, 2001, v. 43 suppl. 1, p. S131, abstract no. S13-P4en_HK
dc.identifier.issn0012-1592en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96454-
dc.description.abstractThe flexibility and success of conditional gene targeting depends on the availability of tissue or cell lineage specific expression of cre recombinase in mouse mutants. The human cathepsin G (hCG) gene is expressed exclusively in promyelocytes/ promonocytes and encodes a neutral serine protease that is package in the azurophil granules of myeloid cells. With the aim of studying human leukaemia using mutant mouse models, we attempted to generate an early myeloid cell-specific cre recombinase transgenic mouse line, hCG-cre, using the hCG promoter. A DNA construct with the cre gene inserted upstream of the coding region of a hCG genomic fragment was used for transgenic mouse production. To assess the tissue specificity and efficiency of the cre recombinase in the hCG-cre mouse line, we would cross the hCG-cre mice with the Z/AP reporter mouse line. The activity and specificity of the alkaline phosphatase reporter in the bone marrow and peripheral blood of the double mutant mice will be examined.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DGDen_HK
dc.relation.ispartofDevelopment, Growth and Differentiationen_HK
dc.titleHuman cathepsinG-cre mice for myeloid-specific gene targetingen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1592&volume=43&issue=Suppl.&spage=S131&epage=&date=2001&atitle=Human+cathepsinG-cre+mice+for+myeloid-specific+gene+targetingen_HK
dc.identifier.emailChan, LC: chanlc@hkucc.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1046/j.1440-169X.2001.00004-Supplement.x-
dc.identifier.hkuros63619en_HK
dc.identifier.volume43en_HK
dc.identifier.issuesuppl.en_HK
dc.identifier.spageS131, abstract no. S13-P4en_HK
dc.identifier.epageS131, abstract no. S13-P4-

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