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Conference Paper: Explant cultures of embryonic rat dorsal root ganglia exhibit heparanase expression in migrating cells

TitleExplant cultures of embryonic rat dorsal root ganglia exhibit heparanase expression in migrating cells
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 150 How to Cite?
AbstractMammalian heparanase is an endo- -D-glucuronidase that cleaves heparan sulfate moieties of proteoglycans at limited sites. Its enzymatic activity is strongly implicated in the regulation of cell growth, migration and differentiation of tumor cells, endothelial cells and inflammatory cells [1] . The findings of the differences in cellular expression of heparanase in normal adult spinal cord [2]open a new area for us to explore the functions of heparanase in cell migration, during neural development and regulation of cellular environments in adult neural tissue. Here, we provide in vitro evidence that heparanase expression correlates with cell migration as revealed by model cultures of embryonic rat dorsal root ganglia (DRG). Cells migrating away and cells about to exit the DRG core showed strong expression of heparanase protein and mRNA, while those cells remaining sessile in the DRG center showed low ISH signals. The upregulated expression of heparanase protein and endoglucuronidase activity in explant cultures compared with neat explants suggest heparanase functions as an active enzyme to facilitate the cellular emigration from the dorsal root ganglia in vitro. Acknowledgement: This work is supported by CRCG, The University of Hong Kong, grants 10204997&10205634 to D.K.Y.S. References 1 Vlodavsky I, et al: Nat Med 1999; 5: 793–802. 2 Zhang Y, et al: J Comp Neurol 2005.
Persistent Identifierhttp://hdl.handle.net/10722/96378
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:31:58Z-
dc.date.available2010-09-25T16:31:58Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 150en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/96378-
dc.description.abstractMammalian heparanase is an endo- -D-glucuronidase that cleaves heparan sulfate moieties of proteoglycans at limited sites. Its enzymatic activity is strongly implicated in the regulation of cell growth, migration and differentiation of tumor cells, endothelial cells and inflammatory cells [1] . The findings of the differences in cellular expression of heparanase in normal adult spinal cord [2]open a new area for us to explore the functions of heparanase in cell migration, during neural development and regulation of cellular environments in adult neural tissue. Here, we provide in vitro evidence that heparanase expression correlates with cell migration as revealed by model cultures of embryonic rat dorsal root ganglia (DRG). Cells migrating away and cells about to exit the DRG core showed strong expression of heparanase protein and mRNA, while those cells remaining sessile in the DRG center showed low ISH signals. The upregulated expression of heparanase protein and endoglucuronidase activity in explant cultures compared with neat explants suggest heparanase functions as an active enzyme to facilitate the cellular emigration from the dorsal root ganglia in vitro. Acknowledgement: This work is supported by CRCG, The University of Hong Kong, grants 10204997&10205634 to D.K.Y.S. References 1 Vlodavsky I, et al: Nat Med 1999; 5: 793–802. 2 Zhang Y, et al: J Comp Neurol 2005.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleExplant cultures of embryonic rat dorsal root ganglia exhibit heparanase expression in migrating cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=150&epage=150&date=2006&atitle=Explant+cultures+of+embryonic+rat+dorsal+root+ganglia+exhibit+heparanase+expression+in+migrating+cellsen_HK
dc.identifier.emailZhang, Y: zhangyigoo@gmail.comen_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros129739en_HK
dc.identifier.volume15en_HK
dc.identifier.spage150en_HK
dc.identifier.epage150en_HK

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