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Conference Paper: Heparan sulfates that upregulate regeneration of post-traumatic sciatic nerves of adult guinea pigs

TitleHeparan sulfates that upregulate regeneration of post-traumatic sciatic nerves of adult guinea pigs
Authors
KeywordsHeparan sulfates
nerve regeneration
Issue Date2002
PublisherSociety for Neuroscience
Citation
Neuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 529.15 How to Cite?
AbstractWe reported that supplementation of soluble heparan sulfate (HS) to the fluid regenerative neural environment could restore functional, axonal reconnection of the severed nerve with the target muscle (Eur J Neurosci 11: 1914-26, 1999). The HS supplements used were from guinea-pig sciatic nerve and bovine kidney. Both showed similar efficacy in facilitating resumption of conduction velocity and re-myelination. We then asked if the composition of HS used as supplements differed from those recoverable from post-crush nerve homogenates. Unsaturated disaccharides resulting from digestions of the glycosaminoglycan (GAG) extracts with a mixture of heparitinases I-III were therefore analyzed by anion-exchange HPLC. The HS supplements comprised non-sulfated [ΔHexA-GlcNAc], monosulfated [ΔHexA-GlcNSO3, ΔHexA(2S)-GlcNAc, ΔHexA-GlcNAc(6S)] and disulfated [ΔHexA-GlcNSO3(6S)] forms, among which the non-sulfated form was predominant. Both supernatant and pellet fractions of injured nerves similarly indicated predominance of ΔHexA-GlcNAc but these were far lower in amount than those introduced as supplement. Whereas the pellet fractions of crushed nerves indicated ΔHexA-GlcNSO3(6S) that peaked at 14 days post-injury, no 6-sulfated HS disaccharides were detectable in the supernatant fractions. The results suggest the importance of non-sulfated forms in stabilizing matrix organization and transient enrichment of 6-sulfated forms of HS in fine-tuning the environment for growth factor-mediated cellular processes. Our HS supplements were therefore timely in providing the required S-domains of HS for tissue reorganization and axonal regrowth (Supported by RGC grant HKU7249/97M).
Persistent Identifierhttp://hdl.handle.net/10722/96271

 

DC FieldValueLanguage
dc.contributor.authorChau, CHen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:28:39Z-
dc.date.available2010-09-25T16:28:39Z-
dc.date.issued2002en_HK
dc.identifier.citationNeuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 529.15en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96271-
dc.description.abstractWe reported that supplementation of soluble heparan sulfate (HS) to the fluid regenerative neural environment could restore functional, axonal reconnection of the severed nerve with the target muscle (Eur J Neurosci 11: 1914-26, 1999). The HS supplements used were from guinea-pig sciatic nerve and bovine kidney. Both showed similar efficacy in facilitating resumption of conduction velocity and re-myelination. We then asked if the composition of HS used as supplements differed from those recoverable from post-crush nerve homogenates. Unsaturated disaccharides resulting from digestions of the glycosaminoglycan (GAG) extracts with a mixture of heparitinases I-III were therefore analyzed by anion-exchange HPLC. The HS supplements comprised non-sulfated [ΔHexA-GlcNAc], monosulfated [ΔHexA-GlcNSO3, ΔHexA(2S)-GlcNAc, ΔHexA-GlcNAc(6S)] and disulfated [ΔHexA-GlcNSO3(6S)] forms, among which the non-sulfated form was predominant. Both supernatant and pellet fractions of injured nerves similarly indicated predominance of ΔHexA-GlcNAc but these were far lower in amount than those introduced as supplement. Whereas the pellet fractions of crushed nerves indicated ΔHexA-GlcNSO3(6S) that peaked at 14 days post-injury, no 6-sulfated HS disaccharides were detectable in the supernatant fractions. The results suggest the importance of non-sulfated forms in stabilizing matrix organization and transient enrichment of 6-sulfated forms of HS in fine-tuning the environment for growth factor-mediated cellular processes. Our HS supplements were therefore timely in providing the required S-domains of HS for tissue reorganization and axonal regrowth (Supported by RGC grant HKU7249/97M).-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectHeparan sulfates-
dc.subjectnerve regeneration-
dc.titleHeparan sulfates that upregulate regeneration of post-traumatic sciatic nerves of adult guinea pigsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.hkuros84408en_HK

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