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Conference Paper: Mapping of chondroitin sulfate glycoform and chondroitin 6-sulfotransferase in neuroepithelial boundaries of mouse embryos

TitleMapping of chondroitin sulfate glycoform and chondroitin 6-sulfotransferase in neuroepithelial boundaries of mouse embryos
Authors
KeywordsRhombomere
chondroitin 6-sulfotransferase
chondroitin 6-sulfate
Issue Date2001
PublisherSociety for Neuroscience
Citation
Neuroscience 2001, San Diego, CA, 10-15 November 2001, Presentation no. 596.17 How to Cite?
AbstractAs chondroitin sulfate (CS) isoforms have been found in the hindbrain region of mouse embryos during early development, we hypothesized that these isoforms are involved in patterning hindbrain segmentation. We chose to study the segmentation pattern in E 8.5 to E 11.5 embryos. Immunostaining with the monoclonal antibody CS-56 revealed immunopositivity in the neuroepithelial boundaries of both tissue sections and whole-mount embryos. The signals were found stronger in E 8.5 and E 9.5 embryos than in later stages. By neutralization with CS isoforms, we found that CS-56 was more sensitive to chondroitin 6-sulfate (C6S) than other CS glycoforms. As C6S is likely a product of chondroitin 6-sulfotransferase (C6ST)-catalyzed reaction, we reasoned that upregulation of C6ST gene expression took place in the boundary regions. A riboprobe specific for the C6ST gene was synthesized and used for in situ hybridization. Signals were found between rhombomeric boundaries in E 8.5 and E 9.5 mouse embryos but not in E 10.5 and E 11.5 embryos. These results imply that C6S was produced by C6ST in the formation or maintenance of the boundaries. Further work will be performed to identify signals that trigger the in situ positive cells to change in C6ST expression and to test the consequence of this change to the formation of rhombomeric boundaries.
Persistent Identifierhttp://hdl.handle.net/10722/96208

 

DC FieldValueLanguage
dc.contributor.authorKwok, JCFen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:26:43Z-
dc.date.available2010-09-25T16:26:43Z-
dc.date.issued2001en_HK
dc.identifier.citationNeuroscience 2001, San Diego, CA, 10-15 November 2001, Presentation no. 596.17en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96208-
dc.description.abstractAs chondroitin sulfate (CS) isoforms have been found in the hindbrain region of mouse embryos during early development, we hypothesized that these isoforms are involved in patterning hindbrain segmentation. We chose to study the segmentation pattern in E 8.5 to E 11.5 embryos. Immunostaining with the monoclonal antibody CS-56 revealed immunopositivity in the neuroepithelial boundaries of both tissue sections and whole-mount embryos. The signals were found stronger in E 8.5 and E 9.5 embryos than in later stages. By neutralization with CS isoforms, we found that CS-56 was more sensitive to chondroitin 6-sulfate (C6S) than other CS glycoforms. As C6S is likely a product of chondroitin 6-sulfotransferase (C6ST)-catalyzed reaction, we reasoned that upregulation of C6ST gene expression took place in the boundary regions. A riboprobe specific for the C6ST gene was synthesized and used for in situ hybridization. Signals were found between rhombomeric boundaries in E 8.5 and E 9.5 mouse embryos but not in E 10.5 and E 11.5 embryos. These results imply that C6S was produced by C6ST in the formation or maintenance of the boundaries. Further work will be performed to identify signals that trigger the in situ positive cells to change in C6ST expression and to test the consequence of this change to the formation of rhombomeric boundaries.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectRhombomere-
dc.subjectchondroitin 6-sulfotransferase-
dc.subjectchondroitin 6-sulfate-
dc.titleMapping of chondroitin sulfate glycoform and chondroitin 6-sulfotransferase in neuroepithelial boundaries of mouse embryosen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailKwok, JCF: h9600218@hkusua.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.hkuros70187en_HK

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