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Conference Paper: Overexpression of epidermal growth factor induced hypospermatogenesis in transgenic mice

TitleOverexpression of epidermal growth factor induced hypospermatogenesis in transgenic mice
Authors
Issue Date2000
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
Citation
The 7th International Congress of Cell Biology, Gold Coast, QLD., Australia, 24–28 September 2000. In Cell Biology International, 2000, v. 24 n. 12, p. 1009-1010 How to Cite?
AbstractThe in vivo role of epidermal growth factor (EGF) is not well defined even though its effects on culture cells were well studied. To understand the developmental, physiological and pathological roles of EGF, we have generated transgenic mice widely expressing human EGF with the use of the b-actin promoter. EGF and transforming growth factor a (TGFa) bind with equal affinity to the EGF receptor, a transmembrane tyrosine kinase, to trigger various biological responses. EGF and TGFa signaling are implicated in the development of the reproductive system. EGF also plays a physiological role in reproduction. Removal of the salivary gland in rodents, which reduces circulating EGF, reduces spermatogenesis, which can be corrected by EGF replacement. Here we show that in our transgenic males, only few post-meiosis II gametes were found, and the mice were sterile. This resembles a common cause of infertility in humans. Furthermore, the transgenic males had reduced serum testosterone. Our findings contrast the previous report on transgenic mice overexpressing TGFa in testis, which showed normal spermatogenesis. These data suggest that EGF is the active ligand for EGF receptor reported in germ cells, and proper EGF expression is important for completion of spermatogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/96170
ISSN
2015 Impact Factor: 1.663
2015 SCImago Journal Rankings: 0.705

 

DC FieldValueLanguage
dc.contributor.authorWong, RWC-
dc.contributor.authorSham, MH-
dc.contributor.authorMak, KKL-
dc.contributor.authorChan, SY-
dc.date.accessioned2010-09-25T16:25:33Z-
dc.date.available2010-09-25T16:25:33Z-
dc.date.issued2000-
dc.identifier.citationThe 7th International Congress of Cell Biology, Gold Coast, QLD., Australia, 24–28 September 2000. In Cell Biology International, 2000, v. 24 n. 12, p. 1009-1010-
dc.identifier.issn1065-6995-
dc.identifier.urihttp://hdl.handle.net/10722/96170-
dc.description.abstractThe in vivo role of epidermal growth factor (EGF) is not well defined even though its effects on culture cells were well studied. To understand the developmental, physiological and pathological roles of EGF, we have generated transgenic mice widely expressing human EGF with the use of the b-actin promoter. EGF and transforming growth factor a (TGFa) bind with equal affinity to the EGF receptor, a transmembrane tyrosine kinase, to trigger various biological responses. EGF and TGFa signaling are implicated in the development of the reproductive system. EGF also plays a physiological role in reproduction. Removal of the salivary gland in rodents, which reduces circulating EGF, reduces spermatogenesis, which can be corrected by EGF replacement. Here we show that in our transgenic males, only few post-meiosis II gametes were found, and the mice were sterile. This resembles a common cause of infertility in humans. Furthermore, the transgenic males had reduced serum testosterone. Our findings contrast the previous report on transgenic mice overexpressing TGFa in testis, which showed normal spermatogenesis. These data suggest that EGF is the active ligand for EGF receptor reported in germ cells, and proper EGF expression is important for completion of spermatogenesis.-
dc.languageeng-
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm-
dc.relation.ispartofCell Biology International-
dc.titleOverexpression of epidermal growth factor induced hypospermatogenesis in transgenic mice-
dc.typeConference_Paper-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1065-6995&volume=24&issue=12&spage=1009&epage=&date=2001&atitle=Overexpression+of+epidermal+growth+factor+induced+hypospermatogenesis+in+transgenic+miceen_HK
dc.identifier.emailSham, MH: mhsham@hku.hk-
dc.identifier.emailChan, SY: sychan@hkucc.hku.hk-
dc.identifier.authoritySham, MH=rp00380-
dc.identifier.authorityChan, SY=rp00356-
dc.description.natureLink_to_subscribed_fulltext-
dc.identifier.doi10.1006/cbir.2000.0671-
dc.identifier.hkuros55815-
dc.identifier.hkuros63608-
dc.identifier.volume24-
dc.identifier.issue12-
dc.identifier.spage1009-
dc.identifier.epage1010-
dc.publisher.placeUnited Kingdom-

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