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Conference Paper: Expression of heparanase in newborn mouse growth plates

TitleExpression of heparanase in newborn mouse growth plates
Authors
Issue Date2005
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0282-0080
Citation
The 18th International Symposium on Glycoconjugates (ISO-18), Florence, Italy, 4-9 September 2005. In Glycoconjugate Journal, , v. 22 n. 4, p. 343, abstract no. P277 How to Cite?
AbstractEndochondral ossification is tightly regulated by various heparan sulphate (HS)-binding growth factors, such as fibroblast growth factor 2 and Indian hedgehog. However, HS chains immobilized as substituents on cell surface or matrix proteoglycans are limited in the capacity to present these growth factors efficiently to their corresponding signaling receptors. We hypothesize that heparanase, an endo-E-Dglucuronidase, cleaves HS chains at specific sites to yield mobile HS fragments and avails HS-binding growth factors to their targets. Here we aim to map the expression pattern of heparanase in growth plates of both Hspg2¨3/¨3 , a HS-deficient perlecan mouse mutant, and its wildtype littermates by immunohistochemistry. In both mutant and wildtype animals, heparanase protein was detectable in both the pericellular and extracellular matrix of the resting and proliferative zones but not detectable in the pre-hypertrophic zone. In particular, heparanase is localized in the extracellular matrix of the hypertrophic zone where von Kossa staining of calcium deposits were also found. Our results suggest little involvement of HS moieties of perlecan in the distribution of heparanase and HS-mediated maturation process of the growth plate.
Persistent Identifierhttp://hdl.handle.net/10722/96158
ISSN
2015 Impact Factor: 1.828
2015 SCImago Journal Rankings: 0.747

 

DC FieldValueLanguage
dc.contributor.authorYeung, MNen_HK
dc.contributor.authorZhou, Zen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:25:11Z-
dc.date.available2010-09-25T16:25:11Z-
dc.date.issued2005en_HK
dc.identifier.citationThe 18th International Symposium on Glycoconjugates (ISO-18), Florence, Italy, 4-9 September 2005. In Glycoconjugate Journal, , v. 22 n. 4, p. 343, abstract no. P277-
dc.identifier.issn0282-0080-
dc.identifier.urihttp://hdl.handle.net/10722/96158-
dc.description.abstractEndochondral ossification is tightly regulated by various heparan sulphate (HS)-binding growth factors, such as fibroblast growth factor 2 and Indian hedgehog. However, HS chains immobilized as substituents on cell surface or matrix proteoglycans are limited in the capacity to present these growth factors efficiently to their corresponding signaling receptors. We hypothesize that heparanase, an endo-E-Dglucuronidase, cleaves HS chains at specific sites to yield mobile HS fragments and avails HS-binding growth factors to their targets. Here we aim to map the expression pattern of heparanase in growth plates of both Hspg2¨3/¨3 , a HS-deficient perlecan mouse mutant, and its wildtype littermates by immunohistochemistry. In both mutant and wildtype animals, heparanase protein was detectable in both the pericellular and extracellular matrix of the resting and proliferative zones but not detectable in the pre-hypertrophic zone. In particular, heparanase is localized in the extracellular matrix of the hypertrophic zone where von Kossa staining of calcium deposits were also found. Our results suggest little involvement of HS moieties of perlecan in the distribution of heparanase and HS-mediated maturation process of the growth plate.-
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0282-0080-
dc.relation.ispartofGlycoconjugate Journalen_HK
dc.rightsThe final publication is available at Springer via http://dx.doi.org/10.1007/s10719-005-3833-1-
dc.titleExpression of heparanase in newborn mouse growth platesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYeung, MN: mandy_ymn@yahoo.com.hken_HK
dc.identifier.emailZhou, Z: zhongjun@hkucc.hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@.hku.hken_HK
dc.identifier.authorityZhou, Z=rp00503en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.doi10.1007/s10719-005-3833-1-
dc.identifier.hkuros114711en_HK
dc.publisher.placeUnited States-

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