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Conference Paper: Heparanse-syndecan-3 interaction in neurons of the adult spinal cord

TitleHeparanse-syndecan-3 interaction in neurons of the adult spinal cord
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006-2007, v. 15 n. 3, p. 133-134 How to Cite?
AbstractHeparan sulfate proteoglycans (HSPGs) are found in the extracellular matrix, on the cell surface and even in intracellular organelles of mammalian neural tissue. The HS moiety can be cleaved endoglycosidically at limited sites by heparanase to yield HS fragments. Most studies of heparanase expression have been related to tissue invasion by tumor cells and inflammatory cells, our study has however been focused on heparanase expression in the normal adult spinal cord. RT-PCR revealed the expression of heparanase mRNA in the spinal cord. In situ hybridization with DIG-labeled anti-sense riboprobe located heparanase mRNA in cells of the spinal cord. Intensely stained signals were found in motor neurons (ventral gray column), interneurons (substantia gelatinosa) and tract cells (dorsal gray column). In the white matter, signals were found mainly in glial cell bodies, such as astrocytes. These results were further confirmed immunocytochemically in combination with in situ hybridization. To find interaction of heparanase with HSPG in spinal cord, we explored spinal cord sections with double immunofluorscence for colocalization of heparanase and syndecan-3. Syndecan-3 was found in neuronal cell bodies of the gray matter, similar to heparanase in cellular distribution. In the white matter, although axon tract were syndecan-3-positivity, there was no detectable heparanase immunoreactivity. No syndecan-3 expression was observable in astrocytes. These observations suggest functional significance of interaction of heparanase and syndecan-3 in the neurons within the normal adult spinal cord.
Persistent Identifierhttp://hdl.handle.net/10722/96154
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorChau, CHen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:25:03Z-
dc.date.available2010-09-25T16:25:03Z-
dc.date.issued2006en_HK
dc.identifier.citationThe Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006-2007, v. 15 n. 3, p. 133-134en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/96154-
dc.description.abstractHeparan sulfate proteoglycans (HSPGs) are found in the extracellular matrix, on the cell surface and even in intracellular organelles of mammalian neural tissue. The HS moiety can be cleaved endoglycosidically at limited sites by heparanase to yield HS fragments. Most studies of heparanase expression have been related to tissue invasion by tumor cells and inflammatory cells, our study has however been focused on heparanase expression in the normal adult spinal cord. RT-PCR revealed the expression of heparanase mRNA in the spinal cord. In situ hybridization with DIG-labeled anti-sense riboprobe located heparanase mRNA in cells of the spinal cord. Intensely stained signals were found in motor neurons (ventral gray column), interneurons (substantia gelatinosa) and tract cells (dorsal gray column). In the white matter, signals were found mainly in glial cell bodies, such as astrocytes. These results were further confirmed immunocytochemically in combination with in situ hybridization. To find interaction of heparanase with HSPG in spinal cord, we explored spinal cord sections with double immunofluorscence for colocalization of heparanase and syndecan-3. Syndecan-3 was found in neuronal cell bodies of the gray matter, similar to heparanase in cellular distribution. In the white matter, although axon tract were syndecan-3-positivity, there was no detectable heparanase immunoreactivity. No syndecan-3 expression was observable in astrocytes. These observations suggest functional significance of interaction of heparanase and syndecan-3 in the neurons within the normal adult spinal cord.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleHeparanse-syndecan-3 interaction in neurons of the adult spinal corden_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=134&epage=134&date=2006&atitle=Heparanse-syndecan-3+interaction+in+neurons+of+the+adult+spinal+corden_HK
dc.identifier.emailZhang, Y: zhangyigoo@gmail.comen_HK
dc.identifier.emailLiu, J: liuj@hkusua.hku.hken_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros129731en_HK
dc.identifier.volume15en_HK
dc.identifier.spage133en_HK
dc.identifier.epage134en_HK

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