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Conference Paper: Commissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfates

TitleCommissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfates
Authors
Issue Date2007
PublisherSociety for Neuroscience
Citation
Neuroscience 2007, San Diego, CA, 3-7 November 2007, Program#/Poster#: 870.6/C12 How to Cite?
AbstractTo determine if chondroitin sulfates (CS) contribute restrictive guidance in commissural projections from the vestibular nucleus, we treated embryos (E12.5-14.5, Sprague-Dawley rats) with chondroitinase ABC or vehicle via the fourth ventricle for 24 hrs in culture and then tracked projections following DiI injection into the vestibular nucleus on one side. After 1 day in vitro (DIV), control treatment resulted in projections that were similar to those that developed in vivo. The projections remained fasciculated as they approached the midline (E12.5+1DIV), crossed the floor plate (E13.5+1DIV), and reached the contralateral nucleus (E14.5+1DIV). In enzyme- treated embryos, unfasciculated projections diverted from the course of the fasciculated pioneer-projections. To determine if the restrictive guidance is due to modulations in sulfation of chondroitins, we determined the mRNA expression profiles of chondroitin sulfotransferases in the hindbrains of E11.5-E16.5 embryos. Semi-quantitative RT-PCR indicated increasing expression as from E11.5 and by E13.5-14.5, peaking of the 6-sulfotransferase mRNA, leveled basal expression of the 4-sulfotransferase-1 mRNA and progressively rising 4-sulfotransferase-2 mRNA were observed. The results highlight roles of CS in fostering axonal fasciculation and limiting stray outgrowth and suggest involvement of chondroitin sulfotransferases in modulating these roles during development of vestibular commissures.
Persistent Identifierhttp://hdl.handle.net/10722/95972

 

DC FieldValueLanguage
dc.contributor.authorYuen, YLen_HK
dc.contributor.authorKwok, JCFen_HK
dc.contributor.authorLau, JWKen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:19:20Z-
dc.date.available2010-09-25T16:19:20Z-
dc.date.issued2007en_HK
dc.identifier.citationNeuroscience 2007, San Diego, CA, 3-7 November 2007, Program#/Poster#: 870.6/C12-
dc.identifier.urihttp://hdl.handle.net/10722/95972-
dc.description.abstractTo determine if chondroitin sulfates (CS) contribute restrictive guidance in commissural projections from the vestibular nucleus, we treated embryos (E12.5-14.5, Sprague-Dawley rats) with chondroitinase ABC or vehicle via the fourth ventricle for 24 hrs in culture and then tracked projections following DiI injection into the vestibular nucleus on one side. After 1 day in vitro (DIV), control treatment resulted in projections that were similar to those that developed in vivo. The projections remained fasciculated as they approached the midline (E12.5+1DIV), crossed the floor plate (E13.5+1DIV), and reached the contralateral nucleus (E14.5+1DIV). In enzyme- treated embryos, unfasciculated projections diverted from the course of the fasciculated pioneer-projections. To determine if the restrictive guidance is due to modulations in sulfation of chondroitins, we determined the mRNA expression profiles of chondroitin sulfotransferases in the hindbrains of E11.5-E16.5 embryos. Semi-quantitative RT-PCR indicated increasing expression as from E11.5 and by E13.5-14.5, peaking of the 6-sulfotransferase mRNA, leveled basal expression of the 4-sulfotransferase-1 mRNA and progressively rising 4-sulfotransferase-2 mRNA were observed. The results highlight roles of CS in fostering axonal fasciculation and limiting stray outgrowth and suggest involvement of chondroitin sulfotransferases in modulating these roles during development of vestibular commissures.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.titleCommissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfatesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYuen, YL: puppy_westie@yahoo.com.hken_HK
dc.identifier.emailKwok, JCF: h9600218@hkusua.hku.hken_HK
dc.identifier.emailLau, JWK: jaegerlau@yahoo.com.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.hkuros149820en_HK

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