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Conference Paper: Adrenomedullin and oviduct function in human and rats

TitleAdrenomedullin and oviduct function in human and rats
Authors
Issue Date2009
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
The 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 suppl. 1, meeting abstract no. 99 How to Cite?
AbstractThe oviduct is the part of the female reproductive tract thatserves the important function to provide a favorable environmentfor sperm maturation, fertilization, and embryo transport. Ourrecent data showed a high level of adrenomedullin (ADM) in theoviduct but the function of this hormone in the oviduct wasnot clear. The aim of our study was to measure the oviductalADM levels in relation to the ovarian cycle and the effect ofADM on the ciliary beat frequency in the rat and human oviduct.Materials and Methods: To study the effect of ADM on the oviduct,4-week-old Sprague-Dawley rats at preovulatory or post-ovulatorystages were killed and the oviducts were slit open longitudinally.The oviducts were incubated for two hours with ADM at 0, 10,100 nM or 100 nM + 1µM human calcitonin-gene-regulatedpeptide receptor antagonist, CGRP8-37. The ciliary beat frequency(CBF) was measured using a photometric method at x100 magnificationat 5 randomly selected fields per tissue strip and expressedin Hz. Human oviducts were collected from women undergoing hysterectomyfor benign disease conditions. Oviductal tissue was treatedwith ADM and/ADM with CGRP8-37 for 2 h and CBF was measuredas described above. To study the effect of sperm on the oviduct,mature epididymal sperm from adult SD rat was collected, washedand incubated in DMEM/F12 for 5 minutes. Motile sperm in themedium was collected, washed and incubated for 5 h for capacitation. 10-week old female rats were monitored for at least two regularestrous cycles. They were killed at proestrus; oviducts wereremoved and slit open and cultured with/without an insert containingcapacitated sperm (2x105) for 24 h. After incubation, a stripof oviduct tissue was taken to measure CBF and the rest of thetissue was extracted to measure ADM peptide by an EIA kit andtotal protein. Results: Our results showed that CBF was higherin the rat oviduct compared with that in human. In both cases,the CBF increased significantly with increasing concentrationof ADM (0, 10, 100 nM) and the increase was greater in the preovulatoryrat oviducts than at postovulatory ones. Both increases wereblocked by ADM receptor antagonist. Spermatozoa coincubationsignificantly enhanced the CBF and ADM production of the oviduct,but the stimulatory effect on ADM production mainly dependedon direct physical contact between the spermatozoa and the oviductalcells. Conclusion: We demonstrated that ADM played a role inthe oviduct function in human and rats. The ciliary beat inthe oviduct was stimulated by ADM produced by the oviductalepithelium. The presence of the sperm up-regulated the ciliarybeat frequency in the oviduct which may affect ova/embryo transportin this part of the reproductive tract. Part of this increasewas due to the increase in ADM secretion produced by the oviductalepithelium as a result of direct contact with the sperm. Thisaction of ADM was mediated by CGRP receptors. Acknowledgement:This work was substantially support by a grant from the ResearchGrants Council of the Hong Kong Special Administrative Region,China (HKU7736/07M).
DescriptionPlatform Session 11
Persistent Identifierhttp://hdl.handle.net/10722/95090
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646

 

DC FieldValueLanguage
dc.contributor.authorO, WSen_HK
dc.contributor.authorLiao, Sen_HK
dc.contributor.authorSun, JZen_HK
dc.contributor.authorHo, JCMen_HK
dc.contributor.authorChiu, CNen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLi, RHWen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2010-09-25T15:51:20Z-
dc.date.available2010-09-25T15:51:20Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 42nd Annual Meeting of the Society for the Study of Reproduction (SSR 2009), Pittsburgh, PA., 18-22 July 2009. In Biology of Reproduction, 2009, v. 81 suppl. 1, meeting abstract no. 99en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/95090-
dc.descriptionPlatform Session 11-
dc.description.abstractThe oviduct is the part of the female reproductive tract thatserves the important function to provide a favorable environmentfor sperm maturation, fertilization, and embryo transport. Ourrecent data showed a high level of adrenomedullin (ADM) in theoviduct but the function of this hormone in the oviduct wasnot clear. The aim of our study was to measure the oviductalADM levels in relation to the ovarian cycle and the effect ofADM on the ciliary beat frequency in the rat and human oviduct.Materials and Methods: To study the effect of ADM on the oviduct,4-week-old Sprague-Dawley rats at preovulatory or post-ovulatorystages were killed and the oviducts were slit open longitudinally.The oviducts were incubated for two hours with ADM at 0, 10,100 nM or 100 nM + 1µM human calcitonin-gene-regulatedpeptide receptor antagonist, CGRP8-37. The ciliary beat frequency(CBF) was measured using a photometric method at x100 magnificationat 5 randomly selected fields per tissue strip and expressedin Hz. Human oviducts were collected from women undergoing hysterectomyfor benign disease conditions. Oviductal tissue was treatedwith ADM and/ADM with CGRP8-37 for 2 h and CBF was measuredas described above. To study the effect of sperm on the oviduct,mature epididymal sperm from adult SD rat was collected, washedand incubated in DMEM/F12 for 5 minutes. Motile sperm in themedium was collected, washed and incubated for 5 h for capacitation. 10-week old female rats were monitored for at least two regularestrous cycles. They were killed at proestrus; oviducts wereremoved and slit open and cultured with/without an insert containingcapacitated sperm (2x105) for 24 h. After incubation, a stripof oviduct tissue was taken to measure CBF and the rest of thetissue was extracted to measure ADM peptide by an EIA kit andtotal protein. Results: Our results showed that CBF was higherin the rat oviduct compared with that in human. In both cases,the CBF increased significantly with increasing concentrationof ADM (0, 10, 100 nM) and the increase was greater in the preovulatoryrat oviducts than at postovulatory ones. Both increases wereblocked by ADM receptor antagonist. Spermatozoa coincubationsignificantly enhanced the CBF and ADM production of the oviduct,but the stimulatory effect on ADM production mainly dependedon direct physical contact between the spermatozoa and the oviductalcells. Conclusion: We demonstrated that ADM played a role inthe oviduct function in human and rats. The ciliary beat inthe oviduct was stimulated by ADM produced by the oviductalepithelium. The presence of the sperm up-regulated the ciliarybeat frequency in the oviduct which may affect ova/embryo transportin this part of the reproductive tract. Part of this increasewas due to the increase in ADM secretion produced by the oviductalepithelium as a result of direct contact with the sperm. Thisaction of ADM was mediated by CGRP receptors. Acknowledgement:This work was substantially support by a grant from the ResearchGrants Council of the Hong Kong Special Administrative Region,China (HKU7736/07M).-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.titleAdrenomedullin and oviduct function in human and ratsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=81, Suppl. 1, meeting abstract no. 99&spage=&epage=&date=2009&atitle=Adrenomedullin+and+oviduct+function+in+human+and+ratsen_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailLiao, S: lsb776@hotmail.comen_HK
dc.identifier.emailSun, JZ: JZSUN@HKUCC.HKU.HKen_HK
dc.identifier.emailHo, JCM: jhocm@hku.hken_HK
dc.identifier.emailChiu, CN: ccn0106@netvigator.comen_HK
dc.identifier.emailNg, EHY: nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLi, RHW: rhwli@HKUCC.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityHo, JCM=rp00258en_HK
dc.identifier.authorityChiu, CN=rp00424en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.hkuros161222en_HK
dc.identifier.volume81en_HK
dc.identifier.issuesuppl. 1, meeting abstract-

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