File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A gene expression signature of genetic instability in colon cancer

TitleA gene expression signature of genetic instability in colon cancer
Authors
Issue Date2005
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2005, v. 65 n. 20, p. 9200-9205 How to Cite?
AbstractGenetic instability plays a central role in the development and progression of human cancer. Two major classes of genetic instability, microsatellite instability (MSI) and chromosome instability (microsatellite stable; MSS), are best understood in the context of colon cancer, where MSI tumors represent ∼ 15% of cases, and compared with MSS tumors, more often arise in the proximal colon and display favorable clinical outcome. To further explore molecular differences, we profiled gene expression in a set of 18 colon cancer cell lines using cDNA microarrays representing ∼ 21,000 different genes. Supervised analysis identified a robust expression signature distinguishing MSI and MSS samples. As few as eight genes predicted with high accuracy the underlying genetic instability in the original and in three independent sample sets, comprising 13 colon cancer cell lines, 61 colorectal tumors, and 87 gastric tumors. Notably, the MSI signature was retained despite genetically correcting the underlying instability, suggesting the signature reflects a legacy of the tumor having arisen from MSI, rather than sensing the ongoing state of MSI. Our findings support a model in which MSI and MSS preferentially target different genes and pathways in cancer. Further, among the MSI signature genes, our findings implicate a role of elevated metallothionein expression in the clinical behavior of MSI cancers. ©2005 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/88796
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGiacomini, CPen_HK
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorYuen, STen_HK
dc.contributor.authorKim, YHen_HK
dc.contributor.authorBair, Een_HK
dc.contributor.authorPollack, JRen_HK
dc.date.accessioned2010-09-06T09:48:06Z-
dc.date.available2010-09-06T09:48:06Z-
dc.date.issued2005en_HK
dc.identifier.citationCancer Research, 2005, v. 65 n. 20, p. 9200-9205en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88796-
dc.description.abstractGenetic instability plays a central role in the development and progression of human cancer. Two major classes of genetic instability, microsatellite instability (MSI) and chromosome instability (microsatellite stable; MSS), are best understood in the context of colon cancer, where MSI tumors represent ∼ 15% of cases, and compared with MSS tumors, more often arise in the proximal colon and display favorable clinical outcome. To further explore molecular differences, we profiled gene expression in a set of 18 colon cancer cell lines using cDNA microarrays representing ∼ 21,000 different genes. Supervised analysis identified a robust expression signature distinguishing MSI and MSS samples. As few as eight genes predicted with high accuracy the underlying genetic instability in the original and in three independent sample sets, comprising 13 colon cancer cell lines, 61 colorectal tumors, and 87 gastric tumors. Notably, the MSI signature was retained despite genetically correcting the underlying instability, suggesting the signature reflects a legacy of the tumor having arisen from MSI, rather than sensing the ongoing state of MSI. Our findings support a model in which MSI and MSS preferentially target different genes and pathways in cancer. Further, among the MSI signature genes, our findings implicate a role of elevated metallothionein expression in the clinical behavior of MSI cancers. ©2005 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.subject.meshAdaptor Proteins, Signal Transducingen_HK
dc.subject.meshCarrier Proteins - geneticsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosomal Instabilityen_HK
dc.subject.meshColonic Neoplasms - classification - genetics - metabolismen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGenomic Instabilityen_HK
dc.subject.meshHCT116 Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshNuclear Proteins - geneticsen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshStomach Neoplasms - genetics - metabolismen_HK
dc.titleA gene expression signature of genetic instability in colon canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=65&issue=20&spage=9200&epage=5&date=2005&atitle=A+Gene+Expression+Signature+of+Genetic+Instability+in+Colon+Canceren_HK
dc.identifier.emailLeung, SY:suetyi@hkucc.hku.hken_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/0008-5472.CAN-04-4163en_HK
dc.identifier.pmid16230380-
dc.identifier.scopuseid_2-s2.0-27144468959en_HK
dc.identifier.hkuros113965en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27144468959&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue20en_HK
dc.identifier.spage9200en_HK
dc.identifier.epage9205en_HK
dc.identifier.isiWOS:000232566800019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike524535-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats