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Article: Abnormal surface markers expression on bone marrow CD34+ cells and correlation with disease activity in patients with systemic lupus erythematosus

TitleAbnormal surface markers expression on bone marrow CD34+ cells and correlation with disease activity in patients with systemic lupus erythematosus
Authors
KeywordsBone morrow CD34+ cells
CD166
Systemic lupus erythematosus
Issue Date2007
PublisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/
Citation
Clinical Rheumatology, 2007, v. 26 n. 12, p. 2073-2079 How to Cite?
Abstract
Defects of hematopoietic stem cells (HSCs) have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the phenotypic characteristics of bone marrow (BM) CD34+ cells in patients with SLE and its relationship with SLE disease activity. Ten SLE patients and 10 healthy subjects were recruited and their BM CD34+ cells were analyzed by flow cytometric analysis with CD45/SSC gating for the expression of CD90, CD95, CD117, CD123, CD164, CD166, FAS-L, and HLA-DR. The percentage of BM CD34+ cells was significantly decreased in active SLE patients (1.48 ± 0.41%, n=7) compared to the healthy controls (2.31 ± 0.75%, n=10, p<0.01), but no significant difference was found between the inactive patients (2.04 ± 0.44%, n=3) and the controls. The expression of CD95, CD123, and CD166 on BM CD34+ cells were significantly increased in SLE patients (48.31 ± 10.59%, 44.9 ± 21.5%, 30.9 ± 19.54%, respectively, n=10) when compared with the control subjects (24.33 ± 11.1%, 19.5 ± 4.4%, 10.7 ± 5.5%, respectively, n=10, p<0.05). The increased CD123 expression was negatively correlated with the number of peripheral white blood cells (r=-0.700, p<0.05, n=10). The percentage of CD166 expression was found significantly correlated with the index of SLE disease activity (r=0.472, p<0.05, n=10) and 24 h proteinuria (r=0.558, p<0.05, n=10), but negatively correlated with serum C3 level (r=-0.712, p<0.01, n=10). Our study found that the surface marker expression of CD95, CD123, and CD166 on BM CD34+ cells were significantly increased in patients. This supports the hypothesis that there are abnormalities of the HSC in SLE. Since CD166 and CD123 correlated with the overall lupus activity, their role as a biomarker of inflammatory disease activity also requires further study. © Clinical Rheumatology 2007.
Persistent Identifierhttp://hdl.handle.net/10722/88771
ISSN
2013 Impact Factor: 1.774
2013 SCImago Journal Rankings: 0.862
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, LYen_HK
dc.contributor.authorZhou, KXen_HK
dc.contributor.authorFeng, XBen_HK
dc.contributor.authorZhang, HYen_HK
dc.contributor.authorDing, XQen_HK
dc.contributor.authorJin, Oen_HK
dc.contributor.authorLu, LWen_HK
dc.contributor.authorLau, CSen_HK
dc.contributor.authorHou, YYen_HK
dc.contributor.authorFan, LMen_HK
dc.date.accessioned2010-09-06T09:47:46Z-
dc.date.available2010-09-06T09:47:46Z-
dc.date.issued2007en_HK
dc.identifier.citationClinical Rheumatology, 2007, v. 26 n. 12, p. 2073-2079en_HK
dc.identifier.issn0770-3198en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88771-
dc.description.abstractDefects of hematopoietic stem cells (HSCs) have been suggested to contribute to the development of systemic lupus erythematosus (SLE). The aim of this study was to investigate the phenotypic characteristics of bone marrow (BM) CD34+ cells in patients with SLE and its relationship with SLE disease activity. Ten SLE patients and 10 healthy subjects were recruited and their BM CD34+ cells were analyzed by flow cytometric analysis with CD45/SSC gating for the expression of CD90, CD95, CD117, CD123, CD164, CD166, FAS-L, and HLA-DR. The percentage of BM CD34+ cells was significantly decreased in active SLE patients (1.48 ± 0.41%, n=7) compared to the healthy controls (2.31 ± 0.75%, n=10, p<0.01), but no significant difference was found between the inactive patients (2.04 ± 0.44%, n=3) and the controls. The expression of CD95, CD123, and CD166 on BM CD34+ cells were significantly increased in SLE patients (48.31 ± 10.59%, 44.9 ± 21.5%, 30.9 ± 19.54%, respectively, n=10) when compared with the control subjects (24.33 ± 11.1%, 19.5 ± 4.4%, 10.7 ± 5.5%, respectively, n=10, p<0.05). The increased CD123 expression was negatively correlated with the number of peripheral white blood cells (r=-0.700, p<0.05, n=10). The percentage of CD166 expression was found significantly correlated with the index of SLE disease activity (r=0.472, p<0.05, n=10) and 24 h proteinuria (r=0.558, p<0.05, n=10), but negatively correlated with serum C3 level (r=-0.712, p<0.01, n=10). Our study found that the surface marker expression of CD95, CD123, and CD166 on BM CD34+ cells were significantly increased in patients. This supports the hypothesis that there are abnormalities of the HSC in SLE. Since CD166 and CD123 correlated with the overall lupus activity, their role as a biomarker of inflammatory disease activity also requires further study. © Clinical Rheumatology 2007.en_HK
dc.languageengen_HK
dc.publisherSpringer-Verlag London Ltd. The Journal's web site is located at http://link.springer.de/link/service/journals/10067/en_HK
dc.relation.ispartofClinical Rheumatologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectBone morrow CD34+ cellsen_HK
dc.subjectCD166en_HK
dc.subjectSystemic lupus erythematosusen_HK
dc.subject.meshAntigens, CD - biosynthesis-
dc.subject.meshAntigens, CD34 - biosynthesis-
dc.subject.meshBone Marrow Cells - immunology - metabolism-
dc.subject.meshCell Adhesion Molecules, Neuronal - biosynthesis-
dc.subject.meshLupus Erythematosus, Systemic - immunology - metabolism - pathology-
dc.titleAbnormal surface markers expression on bone marrow CD34+ cells and correlation with disease activity in patients with systemic lupus erythematosusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0770-3198&volume=26&issue=12&spage=2073&epage=2079&date=2007&atitle=Abnormal+surface+markers+expression+on+bone+marrow+CD34++cells+and+correlation+with+disease+activity+in+patients+with+systemic+lupus+erythematosusen_HK
dc.identifier.emailLu, LW:liweilu@hkucc.hku.hken_HK
dc.identifier.emailLau, CS:cslau@hku.hken_HK
dc.identifier.authorityLu, LW=rp00477en_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10067-007-0621-2en_HK
dc.identifier.pmid17447103en_HK
dc.identifier.scopuseid_2-s2.0-36248958730en_HK
dc.identifier.hkuros132284en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36248958730&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2073en_HK
dc.identifier.epage2079en_HK
dc.identifier.isiWOS:000250833300010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSun, LY=35265069800en_HK
dc.identifier.scopusauthoridZhou, KX=8218830200en_HK
dc.identifier.scopusauthoridFeng, XB=7403047175en_HK
dc.identifier.scopusauthoridZhang, HY=14625777100en_HK
dc.identifier.scopusauthoridDing, XQ=23004378200en_HK
dc.identifier.scopusauthoridJin, O=7004432895en_HK
dc.identifier.scopusauthoridLu, LW=7403963552en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK
dc.identifier.scopusauthoridHou, YY=7402198846en_HK
dc.identifier.scopusauthoridFan, LM=7402513170en_HK

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