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Article: Is immunostaining with HAM56 antibody useful in identifying ovarian origin of metastatic adenocarcinomas?

TitleIs immunostaining with HAM56 antibody useful in identifying ovarian origin of metastatic adenocarcinomas?
Authors
Issue Date1997
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpath
Citation
Human Pathology, 1997, v. 28 n. 1, p. 91-94 How to Cite?
AbstractHAM56 (human alveolar macrophage) is a monoclonal antibody that reacts with macrophages and endothelial cells. There has been controversy as to its usefulness in differentiating adenocarcinomas of ovarian or gastrointestinal origin. The aim of this study is to test the specificity of HAM56 in identifying the origin of metastatic adenocarcinomas. Ninety-two adenocarcinomas of known primary site, metastatic to omentum or lymph nodes were used. Immunostaining for HAM56 was performed on formalin-fixed, paraffin-embedded tissue after antigen retrieval with microwave pretreatment. Positive immunostaining was shown as membrane or cytoplasmic staining with luminal accentuation. Nonspecific staining in necrotic debris or mucin was excluded. Immunoreactivity for HAM56 was found in 37 metastatic adenocarcinomas; 22 of 31 cases (71%) of ovarian origin, 7 of 33 (21%) of colonic origin, 4 of 16 (25%) of gastric origin, 3 of 6 (50%) of biliary origin; and 1 of uterine origin (100%). Negative staining was found in adenocarcinomas from the pancreas (n = 2), cervix (n = 2), and fallopian tube (n = 1). These findings suggest that HAM56 reacts with adenocarcinomas arising from several origins though with a higher frequency in ovarian tumors. It is thus not specific for ovarian carcinomas and is, therefore, not a useful tool to help distinguish adenocarcinomas of unknown origin.
Persistent Identifierhttp://hdl.handle.net/10722/88766
ISSN
2015 Impact Factor: 2.791
2015 SCImago Journal Rankings: 1.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorKhoo, UISen_HK
dc.date.accessioned2010-09-06T09:47:42Z-
dc.date.available2010-09-06T09:47:42Z-
dc.date.issued1997en_HK
dc.identifier.citationHuman Pathology, 1997, v. 28 n. 1, p. 91-94en_HK
dc.identifier.issn0046-8177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88766-
dc.description.abstractHAM56 (human alveolar macrophage) is a monoclonal antibody that reacts with macrophages and endothelial cells. There has been controversy as to its usefulness in differentiating adenocarcinomas of ovarian or gastrointestinal origin. The aim of this study is to test the specificity of HAM56 in identifying the origin of metastatic adenocarcinomas. Ninety-two adenocarcinomas of known primary site, metastatic to omentum or lymph nodes were used. Immunostaining for HAM56 was performed on formalin-fixed, paraffin-embedded tissue after antigen retrieval with microwave pretreatment. Positive immunostaining was shown as membrane or cytoplasmic staining with luminal accentuation. Nonspecific staining in necrotic debris or mucin was excluded. Immunoreactivity for HAM56 was found in 37 metastatic adenocarcinomas; 22 of 31 cases (71%) of ovarian origin, 7 of 33 (21%) of colonic origin, 4 of 16 (25%) of gastric origin, 3 of 6 (50%) of biliary origin; and 1 of uterine origin (100%). Negative staining was found in adenocarcinomas from the pancreas (n = 2), cervix (n = 2), and fallopian tube (n = 1). These findings suggest that HAM56 reacts with adenocarcinomas arising from several origins though with a higher frequency in ovarian tumors. It is thus not specific for ovarian carcinomas and is, therefore, not a useful tool to help distinguish adenocarcinomas of unknown origin.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpathen_HK
dc.relation.ispartofHuman Pathologyen_HK
dc.subject.meshAdenocarcinoma - secondaryen_HK
dc.subject.meshAntibodies, Monoclonalen_HK
dc.subject.meshDiagnosis, Differentialen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMacrophages, Alveolar - immunologyen_HK
dc.subject.meshNeoplasms, Unknown Primary - diagnosisen_HK
dc.subject.meshOvarian Neoplasms - pathologyen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.titleIs immunostaining with HAM56 antibody useful in identifying ovarian origin of metastatic adenocarcinomas?en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0046-8177&volume=28&spage=91&epage=94&date=1997&atitle=Is+immunostaining+with+HAM56+antibody+useful+in+identifying+ovarian+origin+of+metastatic+adenocarcinomas?en_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.emailKhoo, UIS:uskhoo@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityKhoo, UIS=rp00362en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0046-8177(97)90285-9en_HK
dc.identifier.pmid9013838-
dc.identifier.scopuseid_2-s2.0-0031049083en_HK
dc.identifier.hkuros26164en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031049083&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue1en_HK
dc.identifier.spage91en_HK
dc.identifier.epage94en_HK
dc.identifier.isiWOS:A1997WE84200016-
dc.publisher.placeUnited Statesen_HK

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