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Article: Comprehensive analysis of 19q12 amplicon in human gastric cancers

TitleComprehensive analysis of 19q12 amplicon in human gastric cancers
Authors
KeywordsAmplification
Array CGH
Gastric cancer
Microarrays
Issue Date2006
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
Citation
Modern Pathology, 2006, v. 19 n. 6, p. 854-863 How to Cite?
AbstractAmplification at 19q12 has been observed in multiple tumor types, while cyclin E1 (CCNE1) has been considered to be the key oncogene within this amplicon. We have previously applied cDNA microarray analysis to systematically characterize gene expression patterns of gastric tumor and nontumor samples. We identified a cluster of five tightly coregulated genes all located at chromosome 19q12, including CCNE1. We found that the 19q12 gene cluster is highly expressed in gastric tumors compared to nontumor gastric samples. Array based comparative genomic hybridization and real-time PCR was used to define the boundary of the 19q12 amplicon to a region of approximately 200 kb. Interestingly, we found that in some cases amplification at 19q12 was not associated with DNA copy number gain at CCNE1, suggesting that some other genes within the 19q12 amplicon may also have important function during gastric tumorigenesis. We found high expression of the 19q12 gene cluster to be statistically correlated with the cell proliferation gene signature. Using the SAM software, we identified a set of 577 genes whose expression levels positively correlated with the 19q12 gene cluster. GO term analysis revealed that this genelist is enriched with genes involved in cell cycle regulation and cell proliferation. In conclusion, expression array analysis combined with array comparative genomic hybridization and real-time PCR provides a new and powerful tool to identify clusters of genes which may be regulated by genomic DNA aberrations. In addition, our study indicates that amplification at 19q12 is associated with cell proliferation in vivo. © 2006 USCAP, Inc All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88763
ISSN
2015 Impact Factor: 5.485
2015 SCImago Journal Rankings: 2.803
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSuet, YLen_HK
dc.contributor.authorHo, Cen_HK
dc.contributor.authorTu, IPen_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorSo, Sen_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorSiu, TYen_HK
dc.contributor.authorChen, Xen_HK
dc.date.accessioned2010-09-06T09:47:39Z-
dc.date.available2010-09-06T09:47:39Z-
dc.date.issued2006en_HK
dc.identifier.citationModern Pathology, 2006, v. 19 n. 6, p. 854-863en_HK
dc.identifier.issn0893-3952en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88763-
dc.description.abstractAmplification at 19q12 has been observed in multiple tumor types, while cyclin E1 (CCNE1) has been considered to be the key oncogene within this amplicon. We have previously applied cDNA microarray analysis to systematically characterize gene expression patterns of gastric tumor and nontumor samples. We identified a cluster of five tightly coregulated genes all located at chromosome 19q12, including CCNE1. We found that the 19q12 gene cluster is highly expressed in gastric tumors compared to nontumor gastric samples. Array based comparative genomic hybridization and real-time PCR was used to define the boundary of the 19q12 amplicon to a region of approximately 200 kb. Interestingly, we found that in some cases amplification at 19q12 was not associated with DNA copy number gain at CCNE1, suggesting that some other genes within the 19q12 amplicon may also have important function during gastric tumorigenesis. We found high expression of the 19q12 gene cluster to be statistically correlated with the cell proliferation gene signature. Using the SAM software, we identified a set of 577 genes whose expression levels positively correlated with the 19q12 gene cluster. GO term analysis revealed that this genelist is enriched with genes involved in cell cycle regulation and cell proliferation. In conclusion, expression array analysis combined with array comparative genomic hybridization and real-time PCR provides a new and powerful tool to identify clusters of genes which may be regulated by genomic DNA aberrations. In addition, our study indicates that amplification at 19q12 is associated with cell proliferation in vivo. © 2006 USCAP, Inc All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/en_HK
dc.relation.ispartofModern Pathologyen_HK
dc.subjectAmplificationen_HK
dc.subjectArray CGHen_HK
dc.subjectGastric canceren_HK
dc.subjectMicroarraysen_HK
dc.subject.meshAdenocarcinoma - genetics - metabolism - pathologyen_HK
dc.subject.meshChromosomes, Human, Pair 19en_HK
dc.subject.meshCyclin E - genetics - metabolismen_HK
dc.subject.meshDNA, Neoplasm - analysisen_HK
dc.subject.meshGene Amplificationen_HK
dc.subject.meshGene Dosageen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshOncogene Proteins - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshStomach Neoplasms - genetics - metabolism - pathologyen_HK
dc.titleComprehensive analysis of 19q12 amplicon in human gastric cancersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0893-3952&volume=19&spage=854&epage=863&date=2006&atitle=Comprehensive+analysis+of+19Q12+amplicon+in+human+gastric+cancersen_HK
dc.identifier.emailSuet, YL: suetyi@hkucc.hku.hken_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.authoritySuet, YL=rp00359en_HK
dc.identifier.authorityChu, KM=rp00435en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/modpathol.3800593en_HK
dc.identifier.pmid16575401-
dc.identifier.scopuseid_2-s2.0-33646869235en_HK
dc.identifier.hkuros116387en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646869235&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue6en_HK
dc.identifier.spage854en_HK
dc.identifier.epage863en_HK
dc.identifier.isiWOS:000237674100013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSuet, YL=7202044886en_HK
dc.identifier.scopusauthoridHo, C=8914892800en_HK
dc.identifier.scopusauthoridTu, IP=35265351600en_HK
dc.identifier.scopusauthoridLi, R=7404723086en_HK
dc.identifier.scopusauthoridSo, S=7102397384en_HK
dc.identifier.scopusauthoridChu, KM=7402453538en_HK
dc.identifier.scopusauthoridSiu, TY=13612055100en_HK
dc.identifier.scopusauthoridChen, X=8978110800en_HK
dc.identifier.citeulike571893-

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