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Article: Cytogenetic study of malignant ovarian germ cell tumors by chromosome in situ hybridization

TitleCytogenetic study of malignant ovarian germ cell tumors by chromosome in situ hybridization
Authors
KeywordsCISH
Germ cell tumors
Ovary
Issue Date1998
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJG
Citation
International Journal Of Gynecological Cancer, 1998, v. 8 n. 3, p. 222-232 How to Cite?
AbstractScant cytogenetic data are available for ovarian malignant germ cell tumors (MOGCT) when compared with their testicular counterparts. The aim of this study was to analyze numeric and structural chromosome aberrations in MOGCT using the technique of chromosome in situ hybridization (CISH). Twenty- four cases, including six dysgerminomas, 12 yolk sac tumors (YST), and six immature teratomas (IMT) were included. Biotinylated probes specific for the pericentromeric regions of chromosomes 1, 11, 12, 16 and x and the short arm of chromosome 1 (1p) were used on formalin fixed paraffin embedded tumor tissues. All the dysgerminomas and YST showed increase in chromosome copy numbers of the chromosomes analyzed. Gain in chromosomes 1, 12 and 16 were found in all six dysgerminomas. YST showed gain in chromosome 1 (5 cases), chromosome 16 (9 cases), chromosome 12 (11 cases), chromosome 11 (11 cases) as well as chromosome x (8 cases). The hybridization signals for chromosome 12 in three dysgerminomas and four YST were found to be larger and/or smaller than those seen in normal cells suggesting the presence of i(12p). 1p deletion was found in one dysgerminoma and eight YST. In IMT, a gain in chromosome copy number was found only in the neuroepithelium, in chromosome 1 in two cases and chromosome 12 in another two cases. Our data demonstrates that CISH is a useful technique to identify the presence of multiple chromosome aberrations in MOGCT with different patterns seen in different histologic types. Such abnormalities may be important in the biology of these tumors and will be worth further analysis correlating karyotype with clinical behavior.
Persistent Identifierhttp://hdl.handle.net/10722/88753
ISSN
2015 Impact Factor: 2.116
2015 SCImago Journal Rankings: 0.830
References

 

DC FieldValueLanguage
dc.contributor.authorShen, DHen_HK
dc.contributor.authorKhoo, USen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-09-06T09:47:30Z-
dc.date.available2010-09-06T09:47:30Z-
dc.date.issued1998en_HK
dc.identifier.citationInternational Journal Of Gynecological Cancer, 1998, v. 8 n. 3, p. 222-232en_HK
dc.identifier.issn1048-891Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/88753-
dc.description.abstractScant cytogenetic data are available for ovarian malignant germ cell tumors (MOGCT) when compared with their testicular counterparts. The aim of this study was to analyze numeric and structural chromosome aberrations in MOGCT using the technique of chromosome in situ hybridization (CISH). Twenty- four cases, including six dysgerminomas, 12 yolk sac tumors (YST), and six immature teratomas (IMT) were included. Biotinylated probes specific for the pericentromeric regions of chromosomes 1, 11, 12, 16 and x and the short arm of chromosome 1 (1p) were used on formalin fixed paraffin embedded tumor tissues. All the dysgerminomas and YST showed increase in chromosome copy numbers of the chromosomes analyzed. Gain in chromosomes 1, 12 and 16 were found in all six dysgerminomas. YST showed gain in chromosome 1 (5 cases), chromosome 16 (9 cases), chromosome 12 (11 cases), chromosome 11 (11 cases) as well as chromosome x (8 cases). The hybridization signals for chromosome 12 in three dysgerminomas and four YST were found to be larger and/or smaller than those seen in normal cells suggesting the presence of i(12p). 1p deletion was found in one dysgerminoma and eight YST. In IMT, a gain in chromosome copy number was found only in the neuroepithelium, in chromosome 1 in two cases and chromosome 12 in another two cases. Our data demonstrates that CISH is a useful technique to identify the presence of multiple chromosome aberrations in MOGCT with different patterns seen in different histologic types. Such abnormalities may be important in the biology of these tumors and will be worth further analysis correlating karyotype with clinical behavior.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJGen_HK
dc.relation.ispartofInternational Journal of Gynecological Canceren_HK
dc.subjectCISHen_HK
dc.subjectGerm cell tumorsen_HK
dc.subjectOvaryen_HK
dc.titleCytogenetic study of malignant ovarian germ cell tumors by chromosome in situ hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1048-891X&volume=8&spage=222&epage=232&date=1998&atitle=Cytogenetic+study+of+malignant+ovarian+germ+cell+tumors+by+chromosome+in+situ+hybridizationen_HK
dc.identifier.emailKhoo, US:uskhoo@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.authorityKhoo, US=rp00362en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1525-1438.1998.09803.xen_HK
dc.identifier.scopuseid_2-s2.0-0031819660en_HK
dc.identifier.hkuros35067en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031819660&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue3en_HK
dc.identifier.spage222en_HK
dc.identifier.epage232en_HK
dc.publisher.placeUnited Statesen_HK

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