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Article: In situ detection of Epstein-Barr virus in non-small cell lung carcinomas

TitleIn situ detection of Epstein-Barr virus in non-small cell lung carcinomas
Authors
KeywordsClonality
EBNA2
EBV proteins
Epstein-Barr virus
LMP-1
Lymphoepithelioma-like carcinoma
Non-small cell lung carcinoma
Issue Date1995
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal Of Pathology, 1995, v. 177 n. 3, p. 233-240 How to Cite?
AbstractEpstein-Barr virus (EBV) is strongly associated with nasopharyngeal carcinoma and lymphoepithelioma-like carcinomas (LELC) of foregut-derived organs. Recently this group of EBV-associated carcinomas has been expanded by the identification of the virus in conventional adenocarcinomas of the stomach. In situ hybridization (ISH) using a sensitive digoxigenin-labelled EBER RNA probe was performed on 167 consecutive unselected primary non-small cell lung carcinomas, to determine the frequency of EBV association in these tumours. Nine cases (5.4 per cent) showed strong EBER signals in the tumour cell nuclei. By immunohistochemistry, four of the EBER-positive tumours showed patchy expression of the viral latent membrane protein (LMP-1) and none showed any expression of the EBV nuclear antigen 2 (EBNA2). Morphologically, all the positive tumours were LELC, whereas no conventional type of non-small cell lung carcinoma showed EBV association. The LELC presented a morphological spectrum from undifferentiated to squamoid or glandular differentiation. The patients showed a male to female ratio of 8:1. The mean age at presentation was 48 years. Smoking was not a risk factor. All patients were alive at follow-up periods of 23-52 months. Southern blot analysis performed on eight of the nine positive tumours showed a clonal episomal form of EBV, suggesting the clonal expansion of an infected tumour cell early in oncogenesis. These characteristics of the EBV-associated lung tumours justify their consideration as a distinct clinicopathological entity.
Persistent Identifierhttp://hdl.handle.net/10722/88720
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, MPen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorYuen, STen_HK
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorChan, SYen_HK
dc.contributor.authorWang, Een_HK
dc.contributor.authorFu, KHen_HK
dc.date.accessioned2010-09-06T09:47:05Z-
dc.date.available2010-09-06T09:47:05Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Pathology, 1995, v. 177 n. 3, p. 233-240en_HK
dc.identifier.issn0022-3417en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88720-
dc.description.abstractEpstein-Barr virus (EBV) is strongly associated with nasopharyngeal carcinoma and lymphoepithelioma-like carcinomas (LELC) of foregut-derived organs. Recently this group of EBV-associated carcinomas has been expanded by the identification of the virus in conventional adenocarcinomas of the stomach. In situ hybridization (ISH) using a sensitive digoxigenin-labelled EBER RNA probe was performed on 167 consecutive unselected primary non-small cell lung carcinomas, to determine the frequency of EBV association in these tumours. Nine cases (5.4 per cent) showed strong EBER signals in the tumour cell nuclei. By immunohistochemistry, four of the EBER-positive tumours showed patchy expression of the viral latent membrane protein (LMP-1) and none showed any expression of the EBV nuclear antigen 2 (EBNA2). Morphologically, all the positive tumours were LELC, whereas no conventional type of non-small cell lung carcinoma showed EBV association. The LELC presented a morphological spectrum from undifferentiated to squamoid or glandular differentiation. The patients showed a male to female ratio of 8:1. The mean age at presentation was 48 years. Smoking was not a risk factor. All patients were alive at follow-up periods of 23-52 months. Southern blot analysis performed on eight of the nine positive tumours showed a clonal episomal form of EBV, suggesting the clonal expansion of an infected tumour cell early in oncogenesis. These characteristics of the EBV-associated lung tumours justify their consideration as a distinct clinicopathological entity.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_HK
dc.relation.ispartofJournal of Pathologyen_HK
dc.rightsJournal of Pathology. Copyright © John Wiley & Sons Ltd.en_HK
dc.subjectClonalityen_HK
dc.subjectEBNA2en_HK
dc.subjectEBV proteinsen_HK
dc.subjectEpstein-Barr virusen_HK
dc.subjectLMP-1en_HK
dc.subjectLymphoepithelioma-like carcinomaen_HK
dc.subjectNon-small cell lung carcinomaen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshCarcinoma, Non-Small-Cell Lung - virologyen_HK
dc.subject.meshCarcinoma, Squamous Cell - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHerpesviridae Infections - complicationsen_HK
dc.subject.meshHerpesvirus 4, Human - isolation & purificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshLung Neoplasms - virologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshTumor Virus Infections - complicationsen_HK
dc.titleIn situ detection of Epstein-Barr virus in non-small cell lung carcinomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3417&volume=177&spage=233&epage=240&date=1995&atitle=In+situ+detection+of+Epstein-Barr+virus+in+non-small+cell+lung+carcinomasen_HK
dc.identifier.emailWong, MP: mwpik@hkucc.hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.emailLeung, SY: suetyi@hku.hken_HK
dc.identifier.authorityWong, MP=rp00348en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/path.1711770304en_HK
dc.identifier.pmid8551384-
dc.identifier.scopuseid_2-s2.0-0028867314en_HK
dc.identifier.hkuros9738en_HK
dc.identifier.volume177en_HK
dc.identifier.issue3en_HK
dc.identifier.spage233en_HK
dc.identifier.epage240en_HK
dc.identifier.isiWOS:A1995TF18400003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, MP=7403907887en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridYuen, ST=7103160927en_HK
dc.identifier.scopusauthoridLeung, SY=7202044886en_HK
dc.identifier.scopusauthoridChan, SY=7404255845en_HK
dc.identifier.scopusauthoridWang, E=7403414620en_HK
dc.identifier.scopusauthoridFu, KH=7202283800en_HK

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