File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Membrane type 1-matrix metalloproteinase is involved in the migration of human monocyte-derived dendritic cells

TitleMembrane type 1-matrix metalloproteinase is involved in the migration of human monocyte-derived dendritic cells
Authors
KeywordsCell migration
Dendritic cell
Matrix metalloproteinase
Issue Date2006
PublisherNature Publishing Group.
Citation
Immunology And Cell Biology, 2006, v. 84 n. 6, p. 557-562 How to Cite?
AbstractDendritic cells (DC) are highly mobile APC. The trafficking of both immature and mature DC is crucial for their functions, which depends mainly on chemotactic attraction and matrix metalloproteinases (MMP) activity. MMP that are in a transmembrane form belong to membrane type (MT)-MMP, among which MT1-MMP has been shown to possess strong proteolytic activity that is capable of degrading extracellular matrix molecules. Although it is well established that MMP are zinc-dependent endopeptidases that collectively degrade most components of the extracellular matrix, relatively little is known about MT-MMP-mediated matrix degradation during DC migration. In this study, we showed that MT1-MMP was expressed in human monocyte-derived immature and mature DC by semi-quantitative reverse transcription PCR and western blotting analyses. Moreover, immunofluorescence microscopic studies showed that MT1-MMP was expressed on the membrane surface of DC. Blocking of MT1-MMP activity greatly reduced the invasion capacity of immature DC in Matrigel, whereas mature DC mobility was not affected. Taken together, our results show a novel functional link between MT1-MMP and DC motility and suggest that MT1-MMP may play an important role in modulating the migration of immature DC. © 2006 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/88717
ISSN
2015 Impact Factor: 4.473
2015 SCImago Journal Rankings: 2.542
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, MXen_HK
dc.contributor.authorQu, Xen_HK
dc.contributor.authorKong, BHen_HK
dc.contributor.authorLam, QLKen_HK
dc.contributor.authorShao, QQen_HK
dc.contributor.authorDeng, BPen_HK
dc.contributor.authorKo, KHen_HK
dc.contributor.authorLu, Len_HK
dc.date.accessioned2010-09-06T09:47:03Z-
dc.date.available2010-09-06T09:47:03Z-
dc.date.issued2006en_HK
dc.identifier.citationImmunology And Cell Biology, 2006, v. 84 n. 6, p. 557-562en_HK
dc.identifier.issn0818-9641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88717-
dc.description.abstractDendritic cells (DC) are highly mobile APC. The trafficking of both immature and mature DC is crucial for their functions, which depends mainly on chemotactic attraction and matrix metalloproteinases (MMP) activity. MMP that are in a transmembrane form belong to membrane type (MT)-MMP, among which MT1-MMP has been shown to possess strong proteolytic activity that is capable of degrading extracellular matrix molecules. Although it is well established that MMP are zinc-dependent endopeptidases that collectively degrade most components of the extracellular matrix, relatively little is known about MT-MMP-mediated matrix degradation during DC migration. In this study, we showed that MT1-MMP was expressed in human monocyte-derived immature and mature DC by semi-quantitative reverse transcription PCR and western blotting analyses. Moreover, immunofluorescence microscopic studies showed that MT1-MMP was expressed on the membrane surface of DC. Blocking of MT1-MMP activity greatly reduced the invasion capacity of immature DC in Matrigel, whereas mature DC mobility was not affected. Taken together, our results show a novel functional link between MT1-MMP and DC motility and suggest that MT1-MMP may play an important role in modulating the migration of immature DC. © 2006 The Authors.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group.en_HK
dc.relation.ispartofImmunology and Cell Biologyen_HK
dc.subjectCell migrationen_HK
dc.subjectDendritic cellen_HK
dc.subjectMatrix metalloproteinaseen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshDendritic Cells - metabolism - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMatrix Metalloproteinase 14 - metabolism - physiologyen_HK
dc.subject.meshMonocytes - physiologyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleMembrane type 1-matrix metalloproteinase is involved in the migration of human monocyte-derived dendritic cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0818-9641&volume=84&issue=6&spage=557&epage=62&date=2006&atitle=Membrane+type+1-matrix+metalloproteinase+is+involved+in+the+migration+of+human+monocyte-derived+dendritic+cellsen_HK
dc.identifier.emailLam, QLK: qlam@pathology.hku.hken_HK
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_HK
dc.identifier.authorityLam, QLK=rp00312en_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1711.2006.01465.xen_HK
dc.identifier.pmid16956391-
dc.identifier.scopuseid_2-s2.0-33750466237en_HK
dc.identifier.hkuros125415en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750466237&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume84en_HK
dc.identifier.issue6en_HK
dc.identifier.spage557en_HK
dc.identifier.epage562en_HK
dc.identifier.isiWOS:000241624800009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, MX=13309541800en_HK
dc.identifier.scopusauthoridQu, X=8924791300en_HK
dc.identifier.scopusauthoridKong, BH=7005061628en_HK
dc.identifier.scopusauthoridLam, QLK=8722491000en_HK
dc.identifier.scopusauthoridShao, QQ=23467634400en_HK
dc.identifier.scopusauthoridDeng, BP=14008198200en_HK
dc.identifier.scopusauthoridKo, KH=7202688627en_HK
dc.identifier.scopusauthoridLu, L=7403963552en_HK
dc.identifier.citeulike916047-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats