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Article: Increased cystic fibrosis transmembrane conductance regulator (CFTR) expression in the human hydrosalpinx

TitleIncreased cystic fibrosis transmembrane conductance regulator (CFTR) expression in the human hydrosalpinx
Authors
KeywordsCFTR
Female reproductive tract
Hydrosalpinx
Hydrosalpinx fluid
Infertility
Issue Date2005
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2005, v. 20 n. 5, p. 1228-1234 How to Cite?
AbstractBackground: Hydrosalpinx (HSP), characterized by abnormal fluid accumulation in the Fallopian tube, is one of the main causes of infertility in women; however, the mechanism underlying the formation of hydrosalpinx fluid (HF) remains elusive. The present study investigated the possible involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent chloride channel, in the pathogenesis of hydrosalpinx. Methods: Masson's trichrome staining was used to characterize epithelial transformation in human HSP; RT-PCR, immunohistochemistry and immunofluorescence staining were used for CFTR expression and localization. Results: Masson's trichrome staining showed areas of epithelial transformation, focally attenuated and pseudostratified. Immunostaining showed enhanced CFTR immunoreactivity in the focally attenuated and pseudostratified areas of HSP epithelium. RT-PCR revealed that CFTR expression in HSP was significantly greater than that in normal Fallopian tubes. Conclusions: These results indicate that HSP epithelium undergoes epithelial transformation with elevated CFTR expression, which may lead to increased transepithelial electrolyte and fluid secretion resulting in HF formation. The present findings may lead to the development of new treatment strategies for infertile patients with HSP. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88670
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAjonuma, LCen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorHung, CYen_HK
dc.contributor.authorTsang, LLen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorBritoJones, Cen_HK
dc.contributor.authorLok, IHen_HK
dc.contributor.authorHaines, CJen_HK
dc.contributor.authorChan, HCen_HK
dc.date.accessioned2010-09-06T09:46:25Z-
dc.date.available2010-09-06T09:46:25Z-
dc.date.issued2005en_HK
dc.identifier.citationHuman Reproduction, 2005, v. 20 n. 5, p. 1228-1234en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88670-
dc.description.abstractBackground: Hydrosalpinx (HSP), characterized by abnormal fluid accumulation in the Fallopian tube, is one of the main causes of infertility in women; however, the mechanism underlying the formation of hydrosalpinx fluid (HF) remains elusive. The present study investigated the possible involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent chloride channel, in the pathogenesis of hydrosalpinx. Methods: Masson's trichrome staining was used to characterize epithelial transformation in human HSP; RT-PCR, immunohistochemistry and immunofluorescence staining were used for CFTR expression and localization. Results: Masson's trichrome staining showed areas of epithelial transformation, focally attenuated and pseudostratified. Immunostaining showed enhanced CFTR immunoreactivity in the focally attenuated and pseudostratified areas of HSP epithelium. RT-PCR revealed that CFTR expression in HSP was significantly greater than that in normal Fallopian tubes. Conclusions: These results indicate that HSP epithelium undergoes epithelial transformation with elevated CFTR expression, which may lead to increased transepithelial electrolyte and fluid secretion resulting in HF formation. The present findings may lead to the development of new treatment strategies for infertile patients with HSP. © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectCFTRen_HK
dc.subjectFemale reproductive tracten_HK
dc.subjectHydrosalpinxen_HK
dc.subjectHydrosalpinx fluiden_HK
dc.subjectInfertilityen_HK
dc.subject.meshAdulten_HK
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - genetics - immunology - metabolismen_HK
dc.subject.meshEpithelial Cells - pathologyen_HK
dc.subject.meshFallopian Tube Diseases - complications - genetics - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfertility, Female - genetics - pathologyen_HK
dc.subject.meshReference Valuesen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleIncreased cystic fibrosis transmembrane conductance regulator (CFTR) expression in the human hydrosalpinxen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=20&issue=5&spage=1228&epage=1234&date=2005&atitle=Increased+cystic+fibrosis+transmembrane+conductance+regulator+(CFTR)+expression+in+the+human+hydrosalpinxen_HK
dc.identifier.emailAjonuma, LC: louisca@hkucc.hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityAjonuma, LC=rp00051en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/humrep/deh773en_HK
dc.identifier.pmid15705621-
dc.identifier.scopuseid_2-s2.0-20344374144en_HK
dc.identifier.hkuros98070en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20344374144&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1228en_HK
dc.identifier.epage1234en_HK
dc.identifier.isiWOS:000228636600015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridAjonuma, LC=6602292557en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridHung, CY=8581943100en_HK
dc.identifier.scopusauthoridTsang, LL=7103290955en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridBritoJones, C=8581943400en_HK
dc.identifier.scopusauthoridLok, IH=6701801517en_HK
dc.identifier.scopusauthoridHaines, CJ=35884956100en_HK
dc.identifier.scopusauthoridChan, HC=7403402737en_HK
dc.identifier.citeulike172288-

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