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Article: DLC-1 is deleted in primary hepatocellular carcinoma and exerts inhibitory effects on the proliferation of hepatoma cell lines with deleted DLC-1

TitleDLC-1 is deleted in primary hepatocellular carcinoma and exerts inhibitory effects on the proliferation of hepatoma cell lines with deleted DLC-1
Authors
Issue Date2000
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2000, v. 60 n. 23, p. 6581-6584 How to Cite?
AbstractWe investigated the expression and deletion of DLC-1 (frequently deleted in liver cancer gene), first reported in 1998 and having a high homology with rat p122RhoGAP in hepatocellular carcinoma (HCC). Six (20%) of 30 human HCC samples and 2 (40%) of 5 HCC cell lines were found to have no detectable DLC-1 expression by reverse transcription-PCR. Homozygous DLC-1 deletion was detected by Southern blotting in two of six HCC samples and in both HCC cell lines with no DLC-1 expression. Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. Our findings suggest that DLC-1 may play an important role in hepatocarcinogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/88633
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorLiang, ZDen_HK
dc.contributor.authorCao, Len_HK
dc.contributor.authorLee, TKWen_HK
dc.date.accessioned2010-09-06T09:45:56Z-
dc.date.available2010-09-06T09:45:56Z-
dc.date.issued2000en_HK
dc.identifier.citationCancer Research, 2000, v. 60 n. 23, p. 6581-6584en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88633-
dc.description.abstractWe investigated the expression and deletion of DLC-1 (frequently deleted in liver cancer gene), first reported in 1998 and having a high homology with rat p122RhoGAP in hepatocellular carcinoma (HCC). Six (20%) of 30 human HCC samples and 2 (40%) of 5 HCC cell lines were found to have no detectable DLC-1 expression by reverse transcription-PCR. Homozygous DLC-1 deletion was detected by Southern blotting in two of six HCC samples and in both HCC cell lines with no DLC-1 expression. Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. Our findings suggest that DLC-1 may play an important role in hepatocarcinogenesis.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshCarcinoma, Hepatocellular - genetics - pathologyen_HK
dc.subject.meshCell Division - geneticsen_HK
dc.subject.meshGTPase-Activating Proteinsen_HK
dc.subject.meshGene Deletionen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshHomozygoteen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver Neoplasms - genetics - pathologyen_HK
dc.subject.meshProtein Biosynthesisen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshTumor Suppressor Proteinsen_HK
dc.titleDLC-1 is deleted in primary hepatocellular carcinoma and exerts inhibitory effects on the proliferation of hepatoma cell lines with deleted DLC-1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=60&spage=6581&epage=6584&date=2000&atitle=DLC-1+is+deleted+in+primary+hepatocellular+carcinoma+and+exerts+inhibitory+effects+on+the+proliferation+of+hepatoma+cell+lines+with+deleted+DLC-1en_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.emailLee, TKW:tkwlee@hkucc.hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityLee, TKW=rp00447en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11118037-
dc.identifier.scopuseid_2-s2.0-0034548009en_HK
dc.identifier.hkuros56457en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034548009&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume60en_HK
dc.identifier.issue23en_HK
dc.identifier.spage6581en_HK
dc.identifier.epage6584en_HK
dc.identifier.isiWOS:000165638700007-
dc.publisher.placeUnited Statesen_HK

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