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Article: Caspase-3 regulates cell cycle in B cells: A consequence of substrate specificity

TitleCaspase-3 regulates cell cycle in B cells: A consequence of substrate specificity
Authors
Issue Date2003
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/ni
Citation
Nature Immunology, 2003, v. 4 n. 10, p. 1016-1022 How to Cite?
AbstractCaspases are important for apoptosis but are also involved in mammalian cell survival and cell division. Here we report that caspase-3 is a negative regulator of B cell cycling. Mice deficient in caspase-3 (Casp3-/- mice) have increased numbers of splenic B cells that show normal apoptosis but enhanced proliferation in vivo and hyperproliferation after mitogenic stimulation in vitro. Cdkn1a encodes p21 (also called Waf1 or Cip1), a cyclin-dependent kinase (CDK) inhibitor. Although expression of p21 was increased, CDK activities and proliferating cell nuclear antigen (PCNA) were increased in Casp3-/- B cells. Using Casp3-/-Cdkn1a-/- mice, we show that the hyperproliferation of Casp3-/- B cells is abolished when Cdkn1a is also deleted. Our genetic and biochemical data demonstrate that caspase-3 is essential in the regulation of B cell homeostasis.
Persistent Identifierhttp://hdl.handle.net/10722/88597
ISSN
2015 Impact Factor: 19.381
2015 SCImago Journal Rankings: 13.253
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, Men_HK
dc.contributor.authorHakem, Ren_HK
dc.contributor.authorFurlonger, Cen_HK
dc.contributor.authorHakem, Aen_HK
dc.contributor.authorDuncan, GSen_HK
dc.contributor.authorSasaki, Ten_HK
dc.contributor.authorBouchard, Den_HK
dc.contributor.authorLu, Len_HK
dc.contributor.authorWu, GEen_HK
dc.contributor.authorPaige, CJen_HK
dc.contributor.authorMak, TWen_HK
dc.date.accessioned2010-09-06T09:45:28Z-
dc.date.available2010-09-06T09:45:28Z-
dc.date.issued2003en_HK
dc.identifier.citationNature Immunology, 2003, v. 4 n. 10, p. 1016-1022en_HK
dc.identifier.issn1529-2908en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88597-
dc.description.abstractCaspases are important for apoptosis but are also involved in mammalian cell survival and cell division. Here we report that caspase-3 is a negative regulator of B cell cycling. Mice deficient in caspase-3 (Casp3-/- mice) have increased numbers of splenic B cells that show normal apoptosis but enhanced proliferation in vivo and hyperproliferation after mitogenic stimulation in vitro. Cdkn1a encodes p21 (also called Waf1 or Cip1), a cyclin-dependent kinase (CDK) inhibitor. Although expression of p21 was increased, CDK activities and proliferating cell nuclear antigen (PCNA) were increased in Casp3-/- B cells. Using Casp3-/-Cdkn1a-/- mice, we show that the hyperproliferation of Casp3-/- B cells is abolished when Cdkn1a is also deleted. Our genetic and biochemical data demonstrate that caspase-3 is essential in the regulation of B cell homeostasis.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nien_HK
dc.relation.ispartofNature Immunologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApoptosis - immunologyen_HK
dc.subject.meshB-Lymphocytes - cytology - enzymology - immunologyen_HK
dc.subject.meshBromodeoxyuridine - metabolismen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspases - genetics - immunologyen_HK
dc.subject.meshCell Cycle - immunologyen_HK
dc.subject.meshCyclin-Dependent Kinase Inhibitor p21en_HK
dc.subject.meshCyclin-Dependent Kinases - immunology - metabolismen_HK
dc.subject.meshCyclins - biosynthesis - immunologyen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLymphocyte Activation - immunologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshProliferating Cell Nuclear Antigen - biosynthesis - immunologyen_HK
dc.subject.meshSpleen - immunology - pathologyen_HK
dc.subject.meshSubstrate Specificityen_HK
dc.titleCaspase-3 regulates cell cycle in B cells: A consequence of substrate specificityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1529-2908&volume=4&issue=10&spage=1016&epage=1022&date=2003&atitle=Caspase-3+regulates+cell+cycle+in+B+cells:+a+consequence+of+substrate+specificityen_HK
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/ni976en_HK
dc.identifier.pmid12970760-
dc.identifier.scopuseid_2-s2.0-0142092617en_HK
dc.identifier.hkuros87275en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0142092617&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume4en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1016en_HK
dc.identifier.epage1022en_HK
dc.identifier.isiWOS:000185590100018-
dc.publisher.placeUnited Statesen_HK

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