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Article: Identification of carboxypeptidase of glutamate like-B as a candidate suppressor in cell growth and metastasis in human hepatocellular carcinoma

TitleIdentification of carboxypeptidase of glutamate like-B as a candidate suppressor in cell growth and metastasis in human hepatocellular carcinoma
Authors
Issue Date2006
PublisherAmerican Association for Cancer Research.
Citation
Clinical Cancer Research, 2006, v. 12 n. 22, p. 6617-6625 How to Cite?
AbstractPurpose: We have previously done large-scale cDNA transfection screening on human hepatocellular carcinoma (HCC) cells and have identified 3,806 cDNA genes that possess the ability of either stimulating or inhibiting cell growth. In this study, we characterized one of these growth suppressor genes, carboxypeptidase of glutamate like-B (CPGL-B), in HCC. Experimental Design: Semiquantitative reverse-transcription PCR was used to examine the expression levels of CPGL-B. The cellular localization and functions of CPGL-B were investigated by enforced expression of CPGL-B in HCC cells. Results: From our previous cDNA transfection screening, we identified a gene named CPGL and its isoform, CPGL-B. With computational analysis, CPGL was located at chromosome 18q22.3 and was a homologue of peptidase family M20. CPGL was expressed in all adult and fetal tissues, whereas its isoform, CPGL-B, lacking exons 3 and 4, was expressed in all fetal tissues but only in liver and placenta of adult tissues. In HCC, CPGL-B was frequently underexpressed (35 of 90, 38.9%) in tumorous tissues compared with the corresponding nontumorous livers. Intriguingly, the underexpression was significantly associated with the presence of venous invasion (P = 0.018) and tumor microsatellite formation (P = 0.004). Stable transfection of CPGL-B in SMMC7721 HCC cells showed significant inhibition in cell viability, colony formation, cell invasion, and tumor formation in nude mice. CPGL-B also down-regulated CXCR3, matrix metalloproteinase 11, and CD44s, which are involved in cell growth and cell migration. Conclusions: These findings suggest that the frequent underexpression of CPGL-B may be associated with cell growth and metastasis of HCC. © 2006 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/88588
ISSN
2014 Impact Factor: 8.722
2014 SCImago Journal Rankings: 4.097
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Pen_HK
dc.contributor.authorChan, DWen_HK
dc.contributor.authorYi, YZen_HK
dc.contributor.authorJin, JLen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorWan, Den_HK
dc.contributor.authorGu, Jen_HK
dc.date.accessioned2010-09-06T09:45:21Z-
dc.date.available2010-09-06T09:45:21Z-
dc.date.issued2006en_HK
dc.identifier.citationClinical Cancer Research, 2006, v. 12 n. 22, p. 6617-6625en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88588-
dc.description.abstractPurpose: We have previously done large-scale cDNA transfection screening on human hepatocellular carcinoma (HCC) cells and have identified 3,806 cDNA genes that possess the ability of either stimulating or inhibiting cell growth. In this study, we characterized one of these growth suppressor genes, carboxypeptidase of glutamate like-B (CPGL-B), in HCC. Experimental Design: Semiquantitative reverse-transcription PCR was used to examine the expression levels of CPGL-B. The cellular localization and functions of CPGL-B were investigated by enforced expression of CPGL-B in HCC cells. Results: From our previous cDNA transfection screening, we identified a gene named CPGL and its isoform, CPGL-B. With computational analysis, CPGL was located at chromosome 18q22.3 and was a homologue of peptidase family M20. CPGL was expressed in all adult and fetal tissues, whereas its isoform, CPGL-B, lacking exons 3 and 4, was expressed in all fetal tissues but only in liver and placenta of adult tissues. In HCC, CPGL-B was frequently underexpressed (35 of 90, 38.9%) in tumorous tissues compared with the corresponding nontumorous livers. Intriguingly, the underexpression was significantly associated with the presence of venous invasion (P = 0.018) and tumor microsatellite formation (P = 0.004). Stable transfection of CPGL-B in SMMC7721 HCC cells showed significant inhibition in cell viability, colony formation, cell invasion, and tumor formation in nude mice. CPGL-B also down-regulated CXCR3, matrix metalloproteinase 11, and CD44s, which are involved in cell growth and cell migration. Conclusions: These findings suggest that the frequent underexpression of CPGL-B may be associated with cell growth and metastasis of HCC. © 2006 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.en_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarcinoma, Hepatocellular - metabolism - pathology - therapyen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCytoplasm - metabolismen_HK
dc.subject.meshDipeptidases - genetics - isolation & purification - physiologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGrowth Inhibitors - isolation & purificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver - metabolismen_HK
dc.subject.meshLiver Neoplasms - metabolism - pathology - therapyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeoplasm Invasivenessen_HK
dc.subject.meshNeoplasm Metastasis - prevention & controlen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshTissue Distributionen_HK
dc.subject.meshTransfection - methodsen_HK
dc.subject.meshXenograft Model Antitumor Assaysen_HK
dc.titleIdentification of carboxypeptidase of glutamate like-B as a candidate suppressor in cell growth and metastasis in human hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=12&issue=22&spage=6617&epage=6625&date=2006&atitle=Identification+of+carboxypeptidase+of+glutamate+like-B+as+a+candidate+suppressor+in+cell+growth+and+metastasis+in+human+hepatocellular+carcinomaen_HK
dc.identifier.emailChan, DW:dwchan@hkucc.hku.hken_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityChan, DW=rp00543en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/1078-0432.CCR-06-1307en_HK
dc.identifier.pmid17121880-
dc.identifier.scopuseid_2-s2.0-33845290581en_HK
dc.identifier.hkuros129940en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845290581&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue22en_HK
dc.identifier.spage6617en_HK
dc.identifier.epage6625en_HK
dc.identifier.isiWOS:000242263300006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, P=14029386200en_HK
dc.identifier.scopusauthoridChan, DW=26533900600en_HK
dc.identifier.scopusauthoridYi, YZ=36755006600en_HK
dc.identifier.scopusauthoridJin, JL=15127452300en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridWan, D=7103411687en_HK
dc.identifier.scopusauthoridGu, J=7403129786en_HK

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