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Article: Involvement of leptin signaling in the survival and maturation of bone marrow-derived dendritic cells

TitleInvolvement of leptin signaling in the survival and maturation of bone marrow-derived dendritic cells
Authors
KeywordsApoptosis
db/db mouse
Dendritic cells
Leptin receptor
Maturation
Issue Date2006
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 2006, v. 36 n. 12, p. 3118-3130 How to Cite?
AbstractPrevious studies demonstrated that lymphocyte development is impaired in leptin receptor (Ob-R)-deficient db/db mice. However, it remains unclear whether or not leptin signaling plays a physiological role in dendritic cell (DC) development and function. In this study, we first detected Ob-R expression in murine DC. Using db/db mice at a pre-diabetic stage, we demonstrate that the total number of DC generated from bone marrow (BM) cultures is significantly lower than in WT controls. Similarly, selective blockade of leptin with a soluble mouse Ob-R chimera (Ob-R:Fc) inhibited DC generation in wild-type BM cultures. The reduced DC yield in db/db BM culture was attributed to significantly increased apoptosis, which was associated with dysregulated expression of Bcl-2 family genes. Moreover, db/db DC displayed markedly reduced expression of co-stimulatory molecules and a Th2-type cytokine profile, with a poor capacity to stimulate allogeneic T cell proliferation. Consistent with their impaired DC phenotype and function, db/db DC showed significantly down-regulated activities of the PI3K/Akt pathway as well as STAT-3 and IκB-α. In conclusion, our findings demonstrate the involvement of leptin signaling in DC survival and maturation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/88567
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, QLKen_HK
dc.contributor.authorLiu, Sen_HK
dc.contributor.authorCao, Xen_HK
dc.contributor.authorLu, Len_HK
dc.date.accessioned2010-09-06T09:45:03Z-
dc.date.available2010-09-06T09:45:03Z-
dc.date.issued2006en_HK
dc.identifier.citationEuropean Journal Of Immunology, 2006, v. 36 n. 12, p. 3118-3130en_HK
dc.identifier.issn0014-2980en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88567-
dc.description.abstractPrevious studies demonstrated that lymphocyte development is impaired in leptin receptor (Ob-R)-deficient db/db mice. However, it remains unclear whether or not leptin signaling plays a physiological role in dendritic cell (DC) development and function. In this study, we first detected Ob-R expression in murine DC. Using db/db mice at a pre-diabetic stage, we demonstrate that the total number of DC generated from bone marrow (BM) cultures is significantly lower than in WT controls. Similarly, selective blockade of leptin with a soluble mouse Ob-R chimera (Ob-R:Fc) inhibited DC generation in wild-type BM cultures. The reduced DC yield in db/db BM culture was attributed to significantly increased apoptosis, which was associated with dysregulated expression of Bcl-2 family genes. Moreover, db/db DC displayed markedly reduced expression of co-stimulatory molecules and a Th2-type cytokine profile, with a poor capacity to stimulate allogeneic T cell proliferation. Consistent with their impaired DC phenotype and function, db/db DC showed significantly down-regulated activities of the PI3K/Akt pathway as well as STAT-3 and IκB-α. In conclusion, our findings demonstrate the involvement of leptin signaling in DC survival and maturation. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_HK
dc.relation.ispartofEuropean Journal of Immunologyen_HK
dc.subjectApoptosisen_HK
dc.subjectdb/db mouseen_HK
dc.subjectDendritic cellsen_HK
dc.subjectLeptin receptoren_HK
dc.subjectMaturationen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Marrow Cells - cytology - metabolismen_HK
dc.subject.meshCell Differentiation - immunologyen_HK
dc.subject.meshCell Survival - immunologyen_HK
dc.subject.meshDendritic Cells - cytology - metabolismen_HK
dc.subject.meshLeptin - physiologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshSignal Transduction - immunologyen_HK
dc.titleInvolvement of leptin signaling in the survival and maturation of bone marrow-derived dendritic cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2980&volume=36&issue=12&spage=3118&epage=30&date=2006&atitle=Involvement+of+leptin+signaling+in+the+survival+and+maturation+of+bone+marrow-derived+dendritic+cellsen_HK
dc.identifier.emailLam, QLK: qlam@pathology.hku.hken_HK
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_HK
dc.identifier.authorityLam, QLK=rp00312en_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/eji.200636602en_HK
dc.identifier.pmid17125143-
dc.identifier.scopuseid_2-s2.0-33845566273en_HK
dc.identifier.hkuros125414en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845566273&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3118en_HK
dc.identifier.epage3130en_HK
dc.identifier.isiWOS:000243078600004-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLam, QLK=8722491000en_HK
dc.identifier.scopusauthoridLiu, S=7409460628en_HK
dc.identifier.scopusauthoridCao, X=7403370836en_HK
dc.identifier.scopusauthoridLu, L=7403963552en_HK

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