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Article: Gene expression profiling by cDNA array in human hepatoma cell line in response to cisplatin treatment

TitleGene expression profiling by cDNA array in human hepatoma cell line in response to cisplatin treatment
Authors
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 2002, v. 70 n. 14, p. 1677-1690 How to Cite?
AbstractGene expression profiling with cDNA array allows simultaneous analysis of the gene expression pattern of a large number of genes and may enhance the investigation of the molecular mechanisms involved in the treatment of hepatocellular carcinoma with cisplatin. We used cDNA array technology to assess the gene expression profiles related to cell cycle regulation and apoptosis in human hepatoma Hep3B cells in response to cisplatin treatment. In Hep3B cells, apoptosis induced by cisplatin was p53-independent, and was associated with up-regulation of cell cycle regulators, pro-apoptotic genes, growth receptors, and genes involved in signal transduction. These included p33ING1, c-Abl, Bax, insulin-like growth factor binding protein 3, Siva, cyclin D1, RhoA, and Raf-1. Down-regulation of cell cycle regulator CDC2 was observed. Semi-quantitative reverse transcription-polymerase chain reaction and/or Western blot analysis performed on seven of these genes confirmed their upregulation of gene expression. Such global analysis of the cytotoxic response to chemotherapeutic drugs may yield insight into the mechanisms of drug action and allow rational design of more effective treatment strategies. © 2002 Published by Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/88516
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, LFen_HK
dc.contributor.authorLee, TKWen_HK
dc.contributor.authorNg, IOLen_HK
dc.date.accessioned2010-09-06T09:44:23Z-
dc.date.available2010-09-06T09:44:23Z-
dc.date.issued2002en_HK
dc.identifier.citationLife Sciences, 2002, v. 70 n. 14, p. 1677-1690en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88516-
dc.description.abstractGene expression profiling with cDNA array allows simultaneous analysis of the gene expression pattern of a large number of genes and may enhance the investigation of the molecular mechanisms involved in the treatment of hepatocellular carcinoma with cisplatin. We used cDNA array technology to assess the gene expression profiles related to cell cycle regulation and apoptosis in human hepatoma Hep3B cells in response to cisplatin treatment. In Hep3B cells, apoptosis induced by cisplatin was p53-independent, and was associated with up-regulation of cell cycle regulators, pro-apoptotic genes, growth receptors, and genes involved in signal transduction. These included p33ING1, c-Abl, Bax, insulin-like growth factor binding protein 3, Siva, cyclin D1, RhoA, and Raf-1. Down-regulation of cell cycle regulator CDC2 was observed. Semi-quantitative reverse transcription-polymerase chain reaction and/or Western blot analysis performed on seven of these genes confirmed their upregulation of gene expression. Such global analysis of the cytotoxic response to chemotherapeutic drugs may yield insight into the mechanisms of drug action and allow rational design of more effective treatment strategies. © 2002 Published by Elsevier Science Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subject.meshAntineoplastic Agents - pharmacologyen_HK
dc.subject.meshApoptosis - drug effects - geneticsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCarcinoma, Hepatocellular - pathologyen_HK
dc.subject.meshCaspases - geneticsen_HK
dc.subject.meshCell Cycle Proteins - geneticsen_HK
dc.subject.meshCisplatin - pharmacologyen_HK
dc.subject.meshDNA Fragmentationen_HK
dc.subject.meshDNA, Complementaryen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGenes, p53en_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Nick-End Labelingen_HK
dc.subject.meshLiver Neoplasms - pathologyen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleGene expression profiling by cDNA array in human hepatoma cell line in response to cisplatin treatmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=70&issue=14&spage=1677&epage=1690&date=2002&atitle=Gene+expression+profiling+by+cDNA+array+in+human+hepatoma+cell+line+in+response+to+cisplatin+treatmenten_HK
dc.identifier.emailLee, TKW:tkwlee@hkucc.hku.hken_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityLee, TKW=rp00447en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0024-3205(01)01554-5en_HK
dc.identifier.pmid11991255-
dc.identifier.scopuseid_2-s2.0-0037154778en_HK
dc.identifier.hkuros69143en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037154778&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume70en_HK
dc.identifier.issue14en_HK
dc.identifier.spage1677en_HK
dc.identifier.epage1690en_HK
dc.identifier.isiWOS:000174750700008-
dc.publisher.placeUnited Statesen_HK

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