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Article: Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines

TitleComprehensive analysis of the gene expression profiles in human gastric cancer cell lines
Authors
Issue Date2002
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2002, v. 21 n. 42, p. 6549-6556 How to Cite?
AbstractGastric adenocarcinoma is one of the major malignancies worldwide. Gastric cell lines have been widely used as the model to study the genetics, pharmacology and biochemistry of gastric cancers. Here we describe a comprehensive survey of the gene expression profiles of 12 gastric carcinoma cell lines, using cDNA microarray with 43 000 clones. For comparison, we also explored the gene expression patterns of 15 cell lines derived from lymphoid, endothelial, stromal and other epithelial cancers. Expression levels of specific genes were validated through comparison to protein expression by immunohistochemistry using cell block arrays. We found sets of genes whose expression corresponds to the molecular signature of each cell type. In the gastric cancer cell lines, apart from genes that are highly expressed corresponding to their common epithelial origin from the gastrointestinal tract, we found marked heterogeneity among the gene expression patterns of these cell lines. Some of the heterogeneity may reflect their underlying molecular characteristics or specific differentiation program. Two putative gastric carcinoma cell lines were found to be B-cell lymphoma, and another one had no epithelial specific gene expression and hence was of doubtful epithelial origin. These cell lines should no longer be used in gastric carcinoma research. In conclusion, our gene expression database can serve as a powerful resource for the study of gastric cancer using these cell lines.
Persistent Identifierhttp://hdl.handle.net/10722/88489
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJi, Jen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorYi Leung, Sen_HK
dc.contributor.authorA Chi, JTen_HK
dc.contributor.authorMan Chu, Ken_HK
dc.contributor.authorTsan Yuen, Sen_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorSy Chan, Aen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorDunphy, Nen_HK
dc.contributor.authorSo, Sen_HK
dc.date.accessioned2010-09-06T09:44:02Z-
dc.date.available2010-09-06T09:44:02Z-
dc.date.issued2002en_HK
dc.identifier.citationOncogene, 2002, v. 21 n. 42, p. 6549-6556en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88489-
dc.description.abstractGastric adenocarcinoma is one of the major malignancies worldwide. Gastric cell lines have been widely used as the model to study the genetics, pharmacology and biochemistry of gastric cancers. Here we describe a comprehensive survey of the gene expression profiles of 12 gastric carcinoma cell lines, using cDNA microarray with 43 000 clones. For comparison, we also explored the gene expression patterns of 15 cell lines derived from lymphoid, endothelial, stromal and other epithelial cancers. Expression levels of specific genes were validated through comparison to protein expression by immunohistochemistry using cell block arrays. We found sets of genes whose expression corresponds to the molecular signature of each cell type. In the gastric cancer cell lines, apart from genes that are highly expressed corresponding to their common epithelial origin from the gastrointestinal tract, we found marked heterogeneity among the gene expression patterns of these cell lines. Some of the heterogeneity may reflect their underlying molecular characteristics or specific differentiation program. Two putative gastric carcinoma cell lines were found to be B-cell lymphoma, and another one had no epithelial specific gene expression and hence was of doubtful epithelial origin. These cell lines should no longer be used in gastric carcinoma research. In conclusion, our gene expression database can serve as a powerful resource for the study of gastric cancer using these cell lines.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subject.meshCell Lineageen_HK
dc.subject.meshEpithelial Cells - metabolismen_HK
dc.subject.meshGastric Mucosa - metabolism - pathologyen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshLymphoma, B-Cell - chemistry - pathologyen_HK
dc.subject.meshNeoplasm Proteins - genetics - metabolismen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshStomach Neoplasms - genetics - metabolismen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleComprehensive analysis of the gene expression profiles in human gastric cancer cell linesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-9232&volume=21&issue=42&spage=6549&epage=6556&date=2002&atitle=Comprehensive+analysis+of+the+gene+expression+profiles+in+human+gastric+cancer+cell+linesen_HK
dc.identifier.emailYi Leung, S:suetyi@hkucc.hku.hken_HK
dc.identifier.authorityYi Leung, S=rp00359en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.onc.1205829en_HK
dc.identifier.pmid12226758-
dc.identifier.scopuseid_2-s2.0-18644377695en_HK
dc.identifier.hkuros77497en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18644377695&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue42en_HK
dc.identifier.spage6549en_HK
dc.identifier.epage6556en_HK
dc.identifier.isiWOS:000177925300015-
dc.publisher.placeUnited Kingdomen_HK

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