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Article: Characterization of epithelial cell culture from human hydrosalpinges and effects of its conditioned medium on embryo development and sperm motility

TitleCharacterization of epithelial cell culture from human hydrosalpinges and effects of its conditioned medium on embryo development and sperm motility
Authors
KeywordsAcrosome reaction
Conditioned medium
Embryo development
Hydrosalpinx
Sperm motility
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2003, v. 18 n. 2, p. 291-298 How to Cite?
AbstractBackground: Recent studies have reported the negative impact of hydrosalpinx on IVF outcome. Toxic effects of hydrosalpinx fluid (HF) have been the main reason for the recommendation of functional surgery, salpingectomy, prior to IVF. The present study characterized hydrosalpinx epithelial cell culture and examined the effects of its conditioned medium (CM) on sperm motility, acrosome reaction and embryo development. Methods: Normal Fallopian tubes (n = 6) and hydrosalpinges (n = 9) were used to prepare epithelial cell culture and CM. Epithelial cell characterization was confirmed using electron microscopy. Sperm motility and acrosome reaction were determined using computer-aided sperm analysis and acrobead assay respectively and embryo development by mouse embryo development assay. Results: The percentage of human motile sperm incubated in hydrosalpinx CM was significantly different from those in normal Fallopian tube (NFT) CM and modified human tubal fluid medium (hTF) (control) (P < 0.05 at 3 h and P < 0.001 at 5 and 24 h), with alteration in movement characteristic, linearity, 24 h after incubation in hydrosalpinx CM (P < 0.05). However, other sperm movement characteristics remained unchanged. Reduced acrosome reaction and poor mouse embryo development were also observed in hydrosalpinx CM but not in NFT CM and hTF. Conclusions: The results suggest that hydrosalpinx epithelial cells may be producing a fluid milieu hostile to sperm and early embryo development. The established epithelial cell culture system may provide a model to further investigate the mechanisms underlying the toxic effects of HF on embryo development and the adverse effects on IVF outcomes.
Persistent Identifierhttp://hdl.handle.net/10722/88485
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAjonuma, LCen_HK
dc.contributor.authorChan, LNen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorKung, LSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorBritonJones, Cen_HK
dc.contributor.authorLok, IHen_HK
dc.contributor.authorHaines, CJen_HK
dc.contributor.authorChan, HCen_HK
dc.date.accessioned2010-09-06T09:43:59Z-
dc.date.available2010-09-06T09:43:59Z-
dc.date.issued2003en_HK
dc.identifier.citationHuman Reproduction, 2003, v. 18 n. 2, p. 291-298en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88485-
dc.description.abstractBackground: Recent studies have reported the negative impact of hydrosalpinx on IVF outcome. Toxic effects of hydrosalpinx fluid (HF) have been the main reason for the recommendation of functional surgery, salpingectomy, prior to IVF. The present study characterized hydrosalpinx epithelial cell culture and examined the effects of its conditioned medium (CM) on sperm motility, acrosome reaction and embryo development. Methods: Normal Fallopian tubes (n = 6) and hydrosalpinges (n = 9) were used to prepare epithelial cell culture and CM. Epithelial cell characterization was confirmed using electron microscopy. Sperm motility and acrosome reaction were determined using computer-aided sperm analysis and acrobead assay respectively and embryo development by mouse embryo development assay. Results: The percentage of human motile sperm incubated in hydrosalpinx CM was significantly different from those in normal Fallopian tube (NFT) CM and modified human tubal fluid medium (hTF) (control) (P < 0.05 at 3 h and P < 0.001 at 5 and 24 h), with alteration in movement characteristic, linearity, 24 h after incubation in hydrosalpinx CM (P < 0.05). However, other sperm movement characteristics remained unchanged. Reduced acrosome reaction and poor mouse embryo development were also observed in hydrosalpinx CM but not in NFT CM and hTF. Conclusions: The results suggest that hydrosalpinx epithelial cells may be producing a fluid milieu hostile to sperm and early embryo development. The established epithelial cell culture system may provide a model to further investigate the mechanisms underlying the toxic effects of HF on embryo development and the adverse effects on IVF outcomes.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectAcrosome reactionen_HK
dc.subjectConditioned mediumen_HK
dc.subjectEmbryo developmenten_HK
dc.subjectHydrosalpinxen_HK
dc.subjectSperm motilityen_HK
dc.subject.meshAcrosome Reaction - drug effectsen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCulture Media, Conditioned - pharmacologyen_HK
dc.subject.meshEmbryonic and Fetal Development - drug effectsen_HK
dc.subject.meshEpithelial Cells - ultrastructureen_HK
dc.subject.meshFallopian Tube Diseases - pathologyen_HK
dc.subject.meshFallopian Tubes - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMice - embryologyen_HK
dc.subject.meshMice, Inbred ICRen_HK
dc.subject.meshMicroscopy, Electronen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshSperm Motility - drug effectsen_HK
dc.titleCharacterization of epithelial cell culture from human hydrosalpinges and effects of its conditioned medium on embryo development and sperm motilityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=18&issue=2&spage=291&epage=298&date=2003&atitle=Characterization+of+epithelial+cell+culture+from+human+hydrosalpinges+and+effects+of+its+conditioned+medium+on+embryo+development+and+sperm+motility+en_HK
dc.identifier.emailAjonuma, LC: louisca@hkucc.hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityAjonuma, LC=rp00051en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/humrep/deg078en_HK
dc.identifier.pmid12571164-
dc.identifier.scopuseid_2-s2.0-0037329374en_HK
dc.identifier.hkuros76883en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037329374&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue2en_HK
dc.identifier.spage291en_HK
dc.identifier.epage298en_HK
dc.identifier.isiWOS:000181040800011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridAjonuma, LC=6602292557en_HK
dc.identifier.scopusauthoridChan, LN=8151044300en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridChow, PH=36917519800en_HK
dc.identifier.scopusauthoridKung, LS=26659465100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridBritonJones, C=6701451221en_HK
dc.identifier.scopusauthoridLok, IH=6701801517en_HK
dc.identifier.scopusauthoridHaines, CJ=35884956100en_HK
dc.identifier.scopusauthoridChan, HC=7403402737en_HK

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