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Article: Regulation of cell survival during B lymphopoiesis: Increased pre-B cell apoptosis in CD24-transgenic mouse bone marrow

TitleRegulation of cell survival during B lymphopoiesis: Increased pre-B cell apoptosis in CD24-transgenic mouse bone marrow
Authors
Issue Date2000
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 2000, v. 30 n. 9, p. 2686-2691 How to Cite?
AbstractCD24 (heat-stable antigen) is expressed in a developmentally regulated fashion by B cell precursors in mouse bone marrow (BM), but its role in B lymphopoiesis remains obscure. A slight overexpression of CD24 in transgenic (Tg) mice leads to depletion of B lymphoid cells in BM. The present study examines whether CD24 is involved in apoptotic selection of B lineage cells under normal microenvironmental conditions in vivo. Double immunofluorescence labeling and flow cytometry have been used to quantitate the apoptotic rates of phenotypically defined B cell populations in BM of CD24-Tg mice. Apoptosis of pre-B cells expressing cytoplasmic μ heavy chains of IgM but lacking surface (s)IgM was increased both ex vivo and in short-term culture, while the number of pre-B cells was halved compared to BM of normal mice. In contrast, B220+μ- pro-B cells and sIgM+ B lymphocytes showed no significant change in either apoptosis or number. The findings provide evidence that CD24 can play a role in vivo in modulating pre-B cell apoptosis, a quality control checkpoint in B cell development.
Persistent Identifierhttp://hdl.handle.net/10722/88460
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Len_HK
dc.contributor.authorChappel, MSen_HK
dc.contributor.authorHumphries, RKen_HK
dc.contributor.authorOsmond, DGen_HK
dc.date.accessioned2010-09-06T09:43:40Z-
dc.date.available2010-09-06T09:43:40Z-
dc.date.issued2000en_HK
dc.identifier.citationEuropean Journal Of Immunology, 2000, v. 30 n. 9, p. 2686-2691en_HK
dc.identifier.issn0014-2980en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88460-
dc.description.abstractCD24 (heat-stable antigen) is expressed in a developmentally regulated fashion by B cell precursors in mouse bone marrow (BM), but its role in B lymphopoiesis remains obscure. A slight overexpression of CD24 in transgenic (Tg) mice leads to depletion of B lymphoid cells in BM. The present study examines whether CD24 is involved in apoptotic selection of B lineage cells under normal microenvironmental conditions in vivo. Double immunofluorescence labeling and flow cytometry have been used to quantitate the apoptotic rates of phenotypically defined B cell populations in BM of CD24-Tg mice. Apoptosis of pre-B cells expressing cytoplasmic μ heavy chains of IgM but lacking surface (s)IgM was increased both ex vivo and in short-term culture, while the number of pre-B cells was halved compared to BM of normal mice. In contrast, B220+μ- pro-B cells and sIgM+ B lymphocytes showed no significant change in either apoptosis or number. The findings provide evidence that CD24 can play a role in vivo in modulating pre-B cell apoptosis, a quality control checkpoint in B cell development.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_HK
dc.relation.ispartofEuropean Journal of Immunologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntigens, CD - physiologyen_HK
dc.subject.meshAntigens, CD24en_HK
dc.subject.meshApoptosisen_HK
dc.subject.meshB-Lymphocytes - physiologyen_HK
dc.subject.meshBone Marrow Cells - physiologyen_HK
dc.subject.meshHematopoiesisen_HK
dc.subject.meshHematopoietic Stem Cells - physiologyen_HK
dc.subject.meshMembrane Glycoproteinsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C3Hen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMice, Transgenicen_HK
dc.titleRegulation of cell survival during B lymphopoiesis: Increased pre-B cell apoptosis in CD24-transgenic mouse bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2980&volume=30&spage=2686&epage=2691&date=2000&atitle=Regulation+of+cell+survival+during+B+lymphopoiesis:+increased+pre-B+cell+apoptosis+in+CD24-transgenic+mouse+bone+marrowen_HK
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1521-4141(200009)30:9<2686::AID-IMMU2686>3.0.CO;2-Fen_HK
dc.identifier.pmid11009103-
dc.identifier.scopuseid_2-s2.0-0033829931en_HK
dc.identifier.hkuros56452en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033829931&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2686en_HK
dc.identifier.epage2691en_HK
dc.identifier.isiWOS:000089223800029-
dc.publisher.placeGermanyen_HK

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