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Article: p53 overexpression is different in Epstein-Barr virus-associated and Epstein-Barr virus-negative carcinoma

Titlep53 overexpression is different in Epstein-Barr virus-associated and Epstein-Barr virus-negative carcinoma
Authors
KeywordsCarcinoma
Epstein-Barr virus
Immunohistochemistry
P53
Issue Date1998
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS
Citation
Histopathology, 1998, v. 33 n. 4, p. 311-317 How to Cite?
AbstractAims: Epstein-Barr virus (EBV) is associated with a subset of gastric and head and neck carcinomas. While p53 mutation and overexpression is common in gastric cancer, in nasopharyngeal carcinoma p53 is overexpressed yet mutation is uncommon, leading to a proposed viral mechanism of p53 upregulation. We examined the expression of p53 protein in 18 EBV-associated gastric carcinomas (EBV-GA) and compared it with 29 age and sex matched EBV- negative gastric carcinomas (EBV0-GA) and 23 non-nasopharyngeal EBV- associated carcinomas (EBV-CAs) arising from various head and neck regions. Methods and results: Using two monoclonal antibodies (DO7 and PAb1801) with microwave pre-treatment, the p53 protein was scored according to the intensity and percentage of positive cells. The EBV0-GA showed a clear cut bimodal distribution of p53 levels, with either homogeneous intense staining of most tumour nuclei, or only very weak expression in a few cells. Nearly all the EBV-GA and EBV-CAs showed a weak to moderate p53 expression, characterized by heterogeneous intensity of staining in a variable proportion of tumour cells. Conclusions: The difference in p53 levels in the EBV0-GA and EBV-GA is statistically significant. The heterogeneous level of p53 in the EBV-GA and EBV-CAs and its difference from the EBV0-GA is suggestive of a nonmutational mechanism of p53 upregulation and underscores the role of the virus in the oncogenic pathway.
Persistent Identifierhttp://hdl.handle.net/10722/88444
ISSN
2015 Impact Factor: 3.425
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorChau, KYen_HK
dc.contributor.authorYuen, STen_HK
dc.contributor.authorChu, KMen_HK
dc.contributor.authorBranicki, FJen_HK
dc.contributor.authorChung, LPen_HK
dc.date.accessioned2010-09-06T09:43:27Z-
dc.date.available2010-09-06T09:43:27Z-
dc.date.issued1998en_HK
dc.identifier.citationHistopathology, 1998, v. 33 n. 4, p. 311-317en_HK
dc.identifier.issn0309-0167en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88444-
dc.description.abstractAims: Epstein-Barr virus (EBV) is associated with a subset of gastric and head and neck carcinomas. While p53 mutation and overexpression is common in gastric cancer, in nasopharyngeal carcinoma p53 is overexpressed yet mutation is uncommon, leading to a proposed viral mechanism of p53 upregulation. We examined the expression of p53 protein in 18 EBV-associated gastric carcinomas (EBV-GA) and compared it with 29 age and sex matched EBV- negative gastric carcinomas (EBV0-GA) and 23 non-nasopharyngeal EBV- associated carcinomas (EBV-CAs) arising from various head and neck regions. Methods and results: Using two monoclonal antibodies (DO7 and PAb1801) with microwave pre-treatment, the p53 protein was scored according to the intensity and percentage of positive cells. The EBV0-GA showed a clear cut bimodal distribution of p53 levels, with either homogeneous intense staining of most tumour nuclei, or only very weak expression in a few cells. Nearly all the EBV-GA and EBV-CAs showed a weak to moderate p53 expression, characterized by heterogeneous intensity of staining in a variable proportion of tumour cells. Conclusions: The difference in p53 levels in the EBV0-GA and EBV-GA is statistically significant. The heterogeneous level of p53 in the EBV-GA and EBV-CAs and its difference from the EBV0-GA is suggestive of a nonmutational mechanism of p53 upregulation and underscores the role of the virus in the oncogenic pathway.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HISen_HK
dc.relation.ispartofHistopathologyen_HK
dc.rightsHistopathology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectCarcinomaen_HK
dc.subjectEpstein-Barr virusen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectP53en_HK
dc.subject.meshCarcinoma - metabolism - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHead and Neck Neoplasms - metabolism - virologyen_HK
dc.subject.meshHerpesvirus 4, Human - isolation & purificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshStomach Neoplasms - metabolism - virologyen_HK
dc.subject.meshTumor Suppressor Protein p53 - metabolismen_HK
dc.titlep53 overexpression is different in Epstein-Barr virus-associated and Epstein-Barr virus-negative carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=33&spage=311&epage=317&date=1998&atitle=p53+overexpression+is+different+in+Epstein-Barr+virus-associated+and+Epstein-Barr+virus-negative+carcinomaen_HK
dc.identifier.emailLeung, SY: suetyi@hku.hken_HK
dc.identifier.emailChu, KM: chukm@hkucc.hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.identifier.authorityChu, KM=rp00435en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9822919-
dc.identifier.scopuseid_2-s2.0-0031768535en_HK
dc.identifier.hkuros40702en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031768535&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue4en_HK
dc.identifier.spage311en_HK
dc.identifier.epage317en_HK
dc.identifier.isiWOS:000076936700003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLeung, SY=7202044886en_HK
dc.identifier.scopusauthoridChau, KY=20533659300en_HK
dc.identifier.scopusauthoridYuen, ST=7103160927en_HK
dc.identifier.scopusauthoridChu, KM=7402453538en_HK
dc.identifier.scopusauthoridBranicki, FJ=7003617514en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK

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