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Article: Induction of apoptosis by cisplatin and its effect on cell cycle-related proteins and cell cycle changes in hepatoma cells

TitleInduction of apoptosis by cisplatin and its effect on cell cycle-related proteins and cell cycle changes in hepatoma cells
Authors
Issue Date2002
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2002, v. 175 n. 1, p. 27-38 How to Cite?
AbstractWe investigated cisplatin-induced apoptosis and the effects on cell cycle-related proteins and cell cycle changes. Two human hepatoma cell lines, HepG2 (with wild-type p53) and Hep3B (with deleted p53), were treated with different concentrations of cisplatin. Cisplatin induced apoptosis in both cell lines as assessed by cell morphology, DNA fragmentation analysis, TdT-mediated dUTP nick end labeling assay and flow cytometry. HepG2 cells were more sensitive to cisplatin than Hep3B. Low-dose cisplatin induced a transient G1 arrest, S phase block and upregulation of p53 and p21WAF1/CIP1 expression in HepG2, but not in Hep3B cells. With cisplatin at a high dose, both cell lines underwent apoptosis that was accompanied by downregulation of p27KIP1 and Bcl-xL. In HepG2, upregulation of p53 and p21WAF1/CIP1 was observed before apoptosis occurred, suggesting that cisplatin-induced apoptosis in HepG2 might be p53-dependent. Expression of Fas was also increased following cisplatin treatment in HepG2. However, there was no induction of p53, p21WAF1/CIP1 and Fas observed in Hep3B cells. In conclusion, cisplatin induced apoptosis in hepatoma cells via both p53-dependent and -independent pathways. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88414
ISSN
2015 Impact Factor: 5.992
2015 SCImago Journal Rankings: 2.331
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, LFen_HK
dc.contributor.authorNg, IOLen_HK
dc.date.accessioned2010-09-06T09:43:03Z-
dc.date.available2010-09-06T09:43:03Z-
dc.date.issued2002en_HK
dc.identifier.citationCancer Letters, 2002, v. 175 n. 1, p. 27-38en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88414-
dc.description.abstractWe investigated cisplatin-induced apoptosis and the effects on cell cycle-related proteins and cell cycle changes. Two human hepatoma cell lines, HepG2 (with wild-type p53) and Hep3B (with deleted p53), were treated with different concentrations of cisplatin. Cisplatin induced apoptosis in both cell lines as assessed by cell morphology, DNA fragmentation analysis, TdT-mediated dUTP nick end labeling assay and flow cytometry. HepG2 cells were more sensitive to cisplatin than Hep3B. Low-dose cisplatin induced a transient G1 arrest, S phase block and upregulation of p53 and p21WAF1/CIP1 expression in HepG2, but not in Hep3B cells. With cisplatin at a high dose, both cell lines underwent apoptosis that was accompanied by downregulation of p27KIP1 and Bcl-xL. In HepG2, upregulation of p53 and p21WAF1/CIP1 was observed before apoptosis occurred, suggesting that cisplatin-induced apoptosis in HepG2 might be p53-dependent. Expression of Fas was also increased following cisplatin treatment in HepG2. However, there was no induction of p53, p21WAF1/CIP1 and Fas observed in Hep3B cells. In conclusion, cisplatin induced apoptosis in hepatoma cells via both p53-dependent and -independent pathways. © 2002 Elsevier Science Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshActins - geneticsen_HK
dc.subject.meshAntigens, CD95 - geneticsen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCarcinoma, Hepatocellular - pathologyen_HK
dc.subject.meshCell Cycle - drug effectsen_HK
dc.subject.meshCell Cycle Proteins - geneticsen_HK
dc.subject.meshCisplatin - toxicityen_HK
dc.subject.meshCyclin-Dependent Kinase Inhibitor p21en_HK
dc.subject.meshCyclins - geneticsen_HK
dc.subject.meshDNA Fragmentationen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Nick-End Labelingen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshLiver Neoplasms - pathologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleInduction of apoptosis by cisplatin and its effect on cell cycle-related proteins and cell cycle changes in hepatoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3835&volume=175&issue=1&spage=27&epage=38&date=2002&atitle=Induction+of+apoptosis+by+cisplatin+and+its+effect+on+cell+cycle-related+proteins+and+cell+cycle+changes+in+hepatoma+cellsen_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0304-3835(01)00720-0en_HK
dc.identifier.pmid11734333-
dc.identifier.scopuseid_2-s2.0-0037049879en_HK
dc.identifier.hkuros66022en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037049879&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume175en_HK
dc.identifier.issue1en_HK
dc.identifier.spage27en_HK
dc.identifier.epage38en_HK
dc.identifier.isiWOS:000173250100004-
dc.publisher.placeIrelanden_HK

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