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Article: Cell cycle proteins and the development of oral squamous cell carcinoma

TitleCell cycle proteins and the development of oral squamous cell carcinoma
Authors
Issue Date1999
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncology
Citation
Oral Oncology, 1999, v. 35 n. 3, p. 333-342 How to Cite?
AbstractExpression of cell cycle regulatory proteins was evaluated in premalignant and malignant oral epithelial lesions, to test the hypothesis that protein regulation of the cell cycle may be altered in the development of oral squamous cell carcinoma. Archived paraffin-embedded specimens (n = 90) from 25 patients with recurrent or persistent lesions were evaluated in immunohistochemically stained sections for cell cycle regulatory proteins p53, Rb, Cyclin D1, p27, and p21. The cell cycle was also evaluated by expression of nuclear protein Ki67. Sections were graded semiquantitatively using a 0 - 3 + scale to indicate the percentage of positively stained cells. The initial histologic diagnosis for 17/25 patients was either focal keratosis, mild dysplasia, or moderate dysplasia; the initial diagnosis for the remaining eight patients ranged from severe dysplasia to moderately differentiated squamous cell carcinoma. Thirty-three of 90 specimens showed positive p53 expression, 11 of which were dysplasias. Eighty-nine of 90 specimens, from all stages of disease, showed positive Rb expression. Twenty- three of 90 specimens showed positive Cyclin D1 expression, typically in the later stages (carcinoma) of a patient's disease. Eighty-four of 90 specimens showed positive p21 expression; while 55 of 90 specimens were positive for p27. In control mucosa, p27 was highly expressed, while Rb and p21 proteins were expressed at relatively low levels; p53 and Cyclin D1 proteins were largely absent. Generally, staining of p53, Rb, p21, and Ki 67 increased with time in serial biopsies, while p27 showed decreased staining with disease progression. These data show that cell cycle regulatory proteins are altered in both premalignant and malignant disease, and that protein phenotypes are heterogeneous. P53 expression is seen early, and Cyclin D1 expression is seen late in the development of oral premalignant and malignant disease. Expression of p53, Rb, p21 and Ki67 increased, while p27 decreased, with disease progression.
Persistent Identifierhttp://hdl.handle.net/10722/88392
ISSN
2015 Impact Factor: 4.286
2015 SCImago Journal Rankings: 1.764
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSchoelch, MLen_HK
dc.contributor.authorRegezi, JAen_HK
dc.contributor.authorDekker, NPen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorMcMillan, Aen_HK
dc.contributor.authorZiober, BLen_HK
dc.contributor.authorLe, QTen_HK
dc.contributor.authorSilverman, Sen_HK
dc.contributor.authorFu, KKen_HK
dc.date.accessioned2010-09-06T09:42:46Z-
dc.date.available2010-09-06T09:42:46Z-
dc.date.issued1999en_HK
dc.identifier.citationOral Oncology, 1999, v. 35 n. 3, p. 333-342en_HK
dc.identifier.issn1368-8375en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88392-
dc.description.abstractExpression of cell cycle regulatory proteins was evaluated in premalignant and malignant oral epithelial lesions, to test the hypothesis that protein regulation of the cell cycle may be altered in the development of oral squamous cell carcinoma. Archived paraffin-embedded specimens (n = 90) from 25 patients with recurrent or persistent lesions were evaluated in immunohistochemically stained sections for cell cycle regulatory proteins p53, Rb, Cyclin D1, p27, and p21. The cell cycle was also evaluated by expression of nuclear protein Ki67. Sections were graded semiquantitatively using a 0 - 3 + scale to indicate the percentage of positively stained cells. The initial histologic diagnosis for 17/25 patients was either focal keratosis, mild dysplasia, or moderate dysplasia; the initial diagnosis for the remaining eight patients ranged from severe dysplasia to moderately differentiated squamous cell carcinoma. Thirty-three of 90 specimens showed positive p53 expression, 11 of which were dysplasias. Eighty-nine of 90 specimens, from all stages of disease, showed positive Rb expression. Twenty- three of 90 specimens showed positive Cyclin D1 expression, typically in the later stages (carcinoma) of a patient's disease. Eighty-four of 90 specimens showed positive p21 expression; while 55 of 90 specimens were positive for p27. In control mucosa, p27 was highly expressed, while Rb and p21 proteins were expressed at relatively low levels; p53 and Cyclin D1 proteins were largely absent. Generally, staining of p53, Rb, p21, and Ki 67 increased with time in serial biopsies, while p27 showed decreased staining with disease progression. These data show that cell cycle regulatory proteins are altered in both premalignant and malignant disease, and that protein phenotypes are heterogeneous. P53 expression is seen early, and Cyclin D1 expression is seen late in the development of oral premalignant and malignant disease. Expression of p53, Rb, p21 and Ki67 increased, while p27 decreased, with disease progression.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncologyen_HK
dc.relation.ispartofOral Oncologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshCarcinoma, Squamous Cell - metabolismen_HK
dc.subject.meshCell Cycle Proteins - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMouth Neoplasms - metabolismen_HK
dc.subject.meshPrecancerous Conditionsen_HK
dc.titleCell cycle proteins and the development of oral squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0964-1955&volume=1999&issue=35&spage=333&epage=342&date=1999&atitle=Cell+cycle+proteins+and+the+development+of+oral+squamous+cell+carcinomaen_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1368-8375(98)00098-0en_HK
dc.identifier.pmid10621856-
dc.identifier.scopuseid_2-s2.0-0033018571en_HK
dc.identifier.hkuros43023en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033018571&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue3en_HK
dc.identifier.spage333en_HK
dc.identifier.epage342en_HK
dc.identifier.isiWOS:000079456300016-
dc.publisher.placeUnited Kingdomen_HK

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