File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Intravirion targeting of a functional anti-human immunodeficiency virus ribozyme directed to pol

TitleIntravirion targeting of a functional anti-human immunodeficiency virus ribozyme directed to pol
Authors
Issue Date2000
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2000, v. 267 n. 2, p. 174-184 How to Cite?
AbstractRibozymes are catalytic RNAs that offer several advantages as specific therapeutic genes against human immunodeficiency virus type 1 (HIV-1). Significant challenges in antiviral uses of ribozymes include (1) how best to express and to deliver this agent and (2) what is the best locale to target ribozymes against HIV-1 RNA. To explore the former, we have previously characterized several vector systems for efficient expression/delivery of anti-HIV-1 ribozymes (Dropulic et al., 1992; Dropulic and Jeang, 1994a; Smith et al., 1997). Here, to investigate an optimal locale for ribozyme-targeting, we asked whether it might be advantageous to direct ribozymes into HIV-1 virions as opposed to the more conventional approach of targeting ribozymes into infected cells. Two series of experiments were performed. First, we demonstrated that ant-HIV-1 ribozymes could indeed be packaged specifically and efficiently into virions. Second, we compared the virus suppressing activity of packageable ribozyme with its counterpart, which cannot be packaged into HIV-1 virions. Our results showed that although both ribozymes cleaved HiV-1 genomic RNA in vitro with equivalent efficiencies, the former ribozyme demonstrated significantly higher virus-suppressing activity than the latter. These findings provide proof-of-principle that to combat productive HIV-1 replication, intravirion targeting is more effective than intracellular targeting of ribozymes. (C) 2000 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/88056
ISSN
2015 Impact Factor: 3.2
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGiordano, Ven_HK
dc.contributor.authorJin, DYen_HK
dc.contributor.authorRekosh, Den_HK
dc.contributor.authorJeang, KTen_HK
dc.date.accessioned2010-09-06T09:38:04Z-
dc.date.available2010-09-06T09:38:04Z-
dc.date.issued2000en_HK
dc.identifier.citationVirology, 2000, v. 267 n. 2, p. 174-184en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88056-
dc.description.abstractRibozymes are catalytic RNAs that offer several advantages as specific therapeutic genes against human immunodeficiency virus type 1 (HIV-1). Significant challenges in antiviral uses of ribozymes include (1) how best to express and to deliver this agent and (2) what is the best locale to target ribozymes against HIV-1 RNA. To explore the former, we have previously characterized several vector systems for efficient expression/delivery of anti-HIV-1 ribozymes (Dropulic et al., 1992; Dropulic and Jeang, 1994a; Smith et al., 1997). Here, to investigate an optimal locale for ribozyme-targeting, we asked whether it might be advantageous to direct ribozymes into HIV-1 virions as opposed to the more conventional approach of targeting ribozymes into infected cells. Two series of experiments were performed. First, we demonstrated that ant-HIV-1 ribozymes could indeed be packaged specifically and efficiently into virions. Second, we compared the virus suppressing activity of packageable ribozyme with its counterpart, which cannot be packaged into HIV-1 virions. Our results showed that although both ribozymes cleaved HiV-1 genomic RNA in vitro with equivalent efficiencies, the former ribozyme demonstrated significantly higher virus-suppressing activity than the latter. These findings provide proof-of-principle that to combat productive HIV-1 replication, intravirion targeting is more effective than intracellular targeting of ribozymes. (C) 2000 Academic Press.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshGene Products, pol - genetics - metabolismen_HK
dc.subject.meshGene Targetingen_HK
dc.subject.meshHIV - genetics - metabolismen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydrolysisen_HK
dc.subject.meshRNA, Catalytic - genetics - metabolismen_HK
dc.subject.meshRNA, Viral - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshVirion - genetics - physiologyen_HK
dc.subject.meshVirus Assembly - geneticsen_HK
dc.titleIntravirion targeting of a functional anti-human immunodeficiency virus ribozyme directed to polen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0042-6822&volume=267&issue=2&spage=174&epage=184&date=2000&atitle=Intravirion+targeting+of+a+functional+anti-human+immunodeficiency+virus+ribozyme+directed+to+polen_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/viro.1999.0112en_HK
dc.identifier.pmid10662613-
dc.identifier.scopuseid_2-s2.0-0034652541en_HK
dc.identifier.hkuros53959en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034652541&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume267en_HK
dc.identifier.issue2en_HK
dc.identifier.spage174en_HK
dc.identifier.epage184en_HK
dc.identifier.isiWOS:000085434000005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGiordano, V=36795391000en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK
dc.identifier.scopusauthoridRekosh, D=7005857181en_HK
dc.identifier.scopusauthoridJeang, KT=7004824803en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats