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Article: p73 Gene expression in ovarian cancer tissues and cell lines

Titlep73 Gene expression in ovarian cancer tissues and cell lines
Authors
Issue Date2000
PublisherAmerican Association for Cancer Research.
Citation
Clinical Cancer Research, 2000, v. 6 n. 10, p. 3910-3915 How to Cite?
AbstractThe p73 gene, a homology of p53, is a new candidate of imprinting and tumor suppressor gene. To investigate the role of p73 in ovarian cancer, we studied the allelic expression in 56 cases of ovarian cancer using StyI polymorphism analysis. We also examined p73 expression by semi-quantitative reverse transcription-PCR as well as by Western blot analysis and DNA methylation study of the CpG island in exon 1 in ovarian cancer tissues and cell lines. Loss of heterozygosity was found in 8.3% (2 of 24) of the cases. Biallelic expression was demonstrated in 91.7% (22 of 24) of the tumor samples, in 70.8% (17 of 24) of the normal samples, and in 1 ovarian cancer cell line. Imbalanced expression and monoallelic expression were found in three and two pairs of matched samples, respectively. Overexpression of p73 was found in advanced ovarian cancer rather than in early-stage disease or in borderline ovarian tumor. No significant difference was found in the p53 expression. Three cell lines with absent p73 protein expression and one tumor sample with monoallelic expression were methylated in the CpG island. Demethylation in SKOV3 cell line using 5-azacytidine can reactivate the expression of this gene in both the mRNA and the protein level. Our results indicated that p73 was not imprinted in most of the ovarian cancer and normal tissues, but it could be involved in the advanced ovarian cancer through overexpression. DNA methylation may contribute to the lack of p73 expression.
Persistent Identifierhttp://hdl.handle.net/10722/87435
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, CLen_HK
dc.contributor.authorIp, SMen_HK
dc.contributor.authorCheng, Den_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:29:37Z-
dc.date.available2010-09-06T09:29:37Z-
dc.date.issued2000en_HK
dc.identifier.citationClinical Cancer Research, 2000, v. 6 n. 10, p. 3910-3915en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87435-
dc.description.abstractThe p73 gene, a homology of p53, is a new candidate of imprinting and tumor suppressor gene. To investigate the role of p73 in ovarian cancer, we studied the allelic expression in 56 cases of ovarian cancer using StyI polymorphism analysis. We also examined p73 expression by semi-quantitative reverse transcription-PCR as well as by Western blot analysis and DNA methylation study of the CpG island in exon 1 in ovarian cancer tissues and cell lines. Loss of heterozygosity was found in 8.3% (2 of 24) of the cases. Biallelic expression was demonstrated in 91.7% (22 of 24) of the tumor samples, in 70.8% (17 of 24) of the normal samples, and in 1 ovarian cancer cell line. Imbalanced expression and monoallelic expression were found in three and two pairs of matched samples, respectively. Overexpression of p73 was found in advanced ovarian cancer rather than in early-stage disease or in borderline ovarian tumor. No significant difference was found in the p53 expression. Three cell lines with absent p73 protein expression and one tumor sample with monoallelic expression were methylated in the CpG island. Demethylation in SKOV3 cell line using 5-azacytidine can reactivate the expression of this gene in both the mRNA and the protein level. Our results indicated that p73 was not imprinted in most of the ovarian cancer and normal tissues, but it could be involved in the advanced ovarian cancer through overexpression. DNA methylation may contribute to the lack of p73 expression.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.en_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.titlep73 Gene expression in ovarian cancer tissues and cell linesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=6&spage=3910&epage=3915&date=2000&atitle=p73+Gene+expression+in+ovarian+cancer+tissues+and+cell+linesen_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11051237-
dc.identifier.scopuseid_2-s2.0-0033754993en_HK
dc.identifier.hkuros56304en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033754993&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3910en_HK
dc.identifier.epage3915en_HK
dc.identifier.isiWOS:000089931600017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, CL=7501960426en_HK
dc.identifier.scopusauthoridIp, SM=55041321600en_HK
dc.identifier.scopusauthoridCheng, D=7402806161en_HK
dc.identifier.scopusauthoridWong, LC=7402092003en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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