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Article: Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor

TitleDetection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor
Authors
KeywordsEndometrial cancer
Laser-captured micro-dissection
Mitochondrial DNA alterations
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/mito
Citation
Mitochondrion, 2007, v. 7 n. 1-2, p. 171-175 How to Cite?
AbstractSomatic mitochondrial DNA (mtDNA) alterations including point mutations and microsatellite instability (MSI) have been frequently detected in human cancers. To further explore the extensiveness of mtDNA alterations, we have analyzed the occurrence of somatic mtDNA mutations in different populations of endometrial cancer cells from the same tumor tissues as compared with adjacent non-tumor cells. Laser-captured micro-dissection was used to harvest endometrial cancer cells from separated areas of the same tumor and adjacent normal cells. Total DNA isolated from micro-dissected cells was PCR amplified and analyzed for mtDNA alterations by polyacrylamide gel electrophoresis and DNA sequencing. Multiple mtDNA alterations were detected in different portions of the same tumor. Different populations of endometrial cancer cells carried different patterns of mtDNA mutations. Interestingly, unlike previous reports, most mutations were found to be heteroplasmic. We have demonstrated the occurrence of hyper-variability of mtDNA alterations in a single piece of tumor tissue. Our observations support the hypothesis that the accumulation of mtDNA alterations is random and expands independently. The data presented here showed the heterogeneity of cancer cells in terms of mtDNA alterations in endometrial cancer. © 2006 Mitochondria Research Society.
Persistent Identifierhttp://hdl.handle.net/10722/87358
ISSN
2015 Impact Factor: 3.647
2015 SCImago Journal Rankings: 1.797
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorXue, WCen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:28:38Z-
dc.date.available2010-09-06T09:28:38Z-
dc.date.issued2007en_HK
dc.identifier.citationMitochondrion, 2007, v. 7 n. 1-2, p. 171-175en_HK
dc.identifier.issn1567-7249en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87358-
dc.description.abstractSomatic mitochondrial DNA (mtDNA) alterations including point mutations and microsatellite instability (MSI) have been frequently detected in human cancers. To further explore the extensiveness of mtDNA alterations, we have analyzed the occurrence of somatic mtDNA mutations in different populations of endometrial cancer cells from the same tumor tissues as compared with adjacent non-tumor cells. Laser-captured micro-dissection was used to harvest endometrial cancer cells from separated areas of the same tumor and adjacent normal cells. Total DNA isolated from micro-dissected cells was PCR amplified and analyzed for mtDNA alterations by polyacrylamide gel electrophoresis and DNA sequencing. Multiple mtDNA alterations were detected in different portions of the same tumor. Different populations of endometrial cancer cells carried different patterns of mtDNA mutations. Interestingly, unlike previous reports, most mutations were found to be heteroplasmic. We have demonstrated the occurrence of hyper-variability of mtDNA alterations in a single piece of tumor tissue. Our observations support the hypothesis that the accumulation of mtDNA alterations is random and expands independently. The data presented here showed the heterogeneity of cancer cells in terms of mtDNA alterations in endometrial cancer. © 2006 Mitochondria Research Society.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/mitoen_HK
dc.relation.ispartofMitochondrionen_HK
dc.rightsMitochondrion. Copyright © Elsevier BV.en_HK
dc.subjectEndometrial canceren_HK
dc.subjectLaser-captured micro-dissectionen_HK
dc.subjectMitochondrial DNA alterationsen_HK
dc.titleDetection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1567-7249&volume=7&spage=171&epage=175&date=2007&atitle=Detection+of+Mosaic+Pattern+of+Mitochondrial+DNA+Alterations+in+Different+Populations+of+Cells+from+the+Same+Endometrial+Tumoren_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mito.2006.11.014en_HK
dc.identifier.pmid17280873-
dc.identifier.scopuseid_2-s2.0-33847662499en_HK
dc.identifier.hkuros131920en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847662499&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage171en_HK
dc.identifier.epage175en_HK
dc.identifier.isiWOS:000245701400026-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWang, Y=8678094600en_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridLiu, VWS=7006405113en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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