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Article: Comparison of two colloidal silica-based sperm separation media with a non-silica-based medium

TitleComparison of two colloidal silica-based sperm separation media with a non-silica-based medium
Authors
KeywordsAcrosome reaction
CASA
Isolate
Ixaprep
Morphology
Motile sperm recovery
Percoll
Sperm separation
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 1999, v. 72 n. 5, p. 796-802 How to Cite?
AbstractObjective: To study and compare the effects of three sperm separation media, two silica-based (Percoll; Pharmacia Biotech AB, Uppsala, Sweden, and Isolate; Irvine Scientific, Santa Ana, CA) and one non-silica-based (Ixaprep; Medicult, Copenhagen, Denmark), on the recovery of progressive motile sperm, the percentage of sperm with normal morphology, various sperm motion characteristics determined by computer-aided sperm analysis, and the percentage of acrosome-reacted sperm. Design: Prospective study. Setting: A university-based assisted reproductive technology center. Patient(s): Male partners of couples attending our infertility clinic. Intervention(s): None. Main Outcome Measure(s): Various semen parameters. Result(s): Both Isolate and Ixaprep resulted in enhanced recovery of motile spermatozoa compared with Percoll. The percentage of sperm with forward progressive motility, the percentage of sperm with normal morphology, and various sperm motion characteristics were similar after the use of Percoll and Isolate and were significantly better than after the use of Ixaprep. The same percentage of acrosome-reacted spermatozoa was observed with all three media. Similar results were observed in both normal and subnormal semen samples. Conclusion(s): The use of Isolate and Ixaprep resulted in better recovery of motile spermatozoa. Other semen parameters were similar with the use of Isolate and Percoll, whereas the use of Ixaprep was associated with lower sperm velocities and fewer morphologically normal spermatozoa.
Persistent Identifierhttp://hdl.handle.net/10722/87213
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMakkar, Gen_HK
dc.contributor.authorNg, HYen_HK
dc.contributor.authorYeung, SBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2010-09-06T09:26:46Z-
dc.date.available2010-09-06T09:26:46Z-
dc.date.issued1999en_HK
dc.identifier.citationFertility And Sterility, 1999, v. 72 n. 5, p. 796-802en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87213-
dc.description.abstractObjective: To study and compare the effects of three sperm separation media, two silica-based (Percoll; Pharmacia Biotech AB, Uppsala, Sweden, and Isolate; Irvine Scientific, Santa Ana, CA) and one non-silica-based (Ixaprep; Medicult, Copenhagen, Denmark), on the recovery of progressive motile sperm, the percentage of sperm with normal morphology, various sperm motion characteristics determined by computer-aided sperm analysis, and the percentage of acrosome-reacted sperm. Design: Prospective study. Setting: A university-based assisted reproductive technology center. Patient(s): Male partners of couples attending our infertility clinic. Intervention(s): None. Main Outcome Measure(s): Various semen parameters. Result(s): Both Isolate and Ixaprep resulted in enhanced recovery of motile spermatozoa compared with Percoll. The percentage of sperm with forward progressive motility, the percentage of sperm with normal morphology, and various sperm motion characteristics were similar after the use of Percoll and Isolate and were significantly better than after the use of Ixaprep. The same percentage of acrosome-reacted spermatozoa was observed with all three media. Similar results were observed in both normal and subnormal semen samples. Conclusion(s): The use of Isolate and Ixaprep resulted in better recovery of motile spermatozoa. Other semen parameters were similar with the use of Isolate and Percoll, whereas the use of Ixaprep was associated with lower sperm velocities and fewer morphologically normal spermatozoa.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectAcrosome reactionen_HK
dc.subjectCASAen_HK
dc.subjectIsolateen_HK
dc.subjectIxaprepen_HK
dc.subjectMorphologyen_HK
dc.subjectMotile sperm recoveryen_HK
dc.subjectPercollen_HK
dc.subjectSperm separationen_HK
dc.titleComparison of two colloidal silica-based sperm separation media with a non-silica-based mediumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=72&issue=5&spage=796&epage=802&date=1999&atitle=Comparison+of+two+colloidal+silica-based+sperm+separation+media+with+a+non-silica-based+mediumen_HK
dc.identifier.emailNg, HY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, SB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityNg, HY=rp00426en_HK
dc.identifier.authorityYeung, SB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(99)00375-1en_HK
dc.identifier.pmid10560980-
dc.identifier.scopuseid_2-s2.0-0032743474en_HK
dc.identifier.hkuros48175en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032743474&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume72en_HK
dc.identifier.issue5en_HK
dc.identifier.spage796en_HK
dc.identifier.epage802en_HK
dc.identifier.isiWOS:000083456000006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMakkar, G=6603413197en_HK
dc.identifier.scopusauthoridNg, HY=35238184300en_HK
dc.identifier.scopusauthoridYeung, SB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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