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Article: Comparison of human papillomavirus DNA levels in gynecological cancers: Implication for cancer development

TitleComparison of human papillomavirus DNA levels in gynecological cancers: Implication for cancer development
Authors
KeywordsCervical cancer
Endometrial cancer
Human papillomavirus
Ovarian cancer
Real-time quantitative PCR
Viral load
Issue Date2003
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBI
Citation
Tumor Biology, 2003, v. 24 n. 6, p. 310-316 How to Cite?
AbstractWe have previously demonstrated the presence of human papillomavirus (HPV) DNA in several gynecological cancers using conventional PCR. In the present study, to further understand the role of HPV in malignant transformation of these cancers, the infection rates and viral loads of HPV 16 and 18 in gynecological cancers were analyzed using real-time quantitative PCR (qPCR). HPV 16 DNA was detected in 61.0% (58/95), 15.2% (7/46) and 32.1% (18/56) of cases of cervical, endometrial and ovarian cancers, respectively. On the other hand, HPV 18 DNA was detected in 23.2% (22/95) of cervical cancers, 1.8% (1/56) of ovarian cancers, and in no cases of endometrial cancer. Thus, HPV 16 is much more prevalent than HPV 18 in malignancies of the female genital tract. We also found that both HPV 16 and 18 were significantly (p < 0.05) less frequently present in endometrial and ovarian cancers than in cervical cancer. The median copy numbers of HPV 16 DNA in endometrial and ovarian cancers were 3,500 and 7,590 copies/μg DNA, respectively. These amounts were also significantly (p < 0.05) lower than HPV 16 DNA in cervical cancer (492,800 copies/μg DNA). Thus, HPV 16 could be detected in all three types of gynecological cancer, whilst HPV 18 is extremely rare in endometrial and ovarian cancers. The lower HPV 16 infection rates and lower copy numbers when compared with cervical cancer tend to suggest that HPV plays a less essential role in the development of endometrial cancer and ovarian cancer. Copyright © 2003 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/87208
ISSN
2015 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.989
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, HJen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorTsang, PCKen_HK
dc.contributor.authorYip, AMWen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:26:43Z-
dc.date.available2010-09-06T09:26:43Z-
dc.date.issued2003en_HK
dc.identifier.citationTumor Biology, 2003, v. 24 n. 6, p. 310-316en_HK
dc.identifier.issn1010-4283en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87208-
dc.description.abstractWe have previously demonstrated the presence of human papillomavirus (HPV) DNA in several gynecological cancers using conventional PCR. In the present study, to further understand the role of HPV in malignant transformation of these cancers, the infection rates and viral loads of HPV 16 and 18 in gynecological cancers were analyzed using real-time quantitative PCR (qPCR). HPV 16 DNA was detected in 61.0% (58/95), 15.2% (7/46) and 32.1% (18/56) of cases of cervical, endometrial and ovarian cancers, respectively. On the other hand, HPV 18 DNA was detected in 23.2% (22/95) of cervical cancers, 1.8% (1/56) of ovarian cancers, and in no cases of endometrial cancer. Thus, HPV 16 is much more prevalent than HPV 18 in malignancies of the female genital tract. We also found that both HPV 16 and 18 were significantly (p < 0.05) less frequently present in endometrial and ovarian cancers than in cervical cancer. The median copy numbers of HPV 16 DNA in endometrial and ovarian cancers were 3,500 and 7,590 copies/μg DNA, respectively. These amounts were also significantly (p < 0.05) lower than HPV 16 DNA in cervical cancer (492,800 copies/μg DNA). Thus, HPV 16 could be detected in all three types of gynecological cancer, whilst HPV 18 is extremely rare in endometrial and ovarian cancers. The lower HPV 16 infection rates and lower copy numbers when compared with cervical cancer tend to suggest that HPV plays a less essential role in the development of endometrial cancer and ovarian cancer. Copyright © 2003 S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBIen_HK
dc.relation.ispartofTumor Biologyen_HK
dc.subjectCervical canceren_HK
dc.subjectEndometrial canceren_HK
dc.subjectHuman papillomavirusen_HK
dc.subjectOvarian canceren_HK
dc.subjectReal-time quantitative PCRen_HK
dc.subjectViral loaden_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshDNA, Viral - analysisen_HK
dc.subject.meshEndometrial Neoplasms - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Dosageen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIncidenceen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOvarian Neoplasms - virologyen_HK
dc.subject.meshPapillomaviridae - genetics - isolation & purificationen_HK
dc.subject.meshPapillomavirus Infections - complications - epidemiology - virologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshUterine Cervical Neoplasms - virologyen_HK
dc.subject.meshViral Loaden_HK
dc.titleComparison of human papillomavirus DNA levels in gynecological cancers: Implication for cancer developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1010-4283&volume=24&issue=6&spage=310&epage=316&date=2003&atitle=Comparison+of+human+papillomavirus+DNA+levels+in+gynecological+cancers:+implication+for+cancer+developmenten_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000076463en_HK
dc.identifier.pmid15004491-
dc.identifier.scopuseid_2-s2.0-1542297658en_HK
dc.identifier.hkuros86892en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1542297658&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue6en_HK
dc.identifier.spage310en_HK
dc.identifier.epage316en_HK
dc.identifier.isiWOS:000220001300006-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridYang, HJ=7408624370en_HK
dc.identifier.scopusauthoridLiu, VWS=7006405113en_HK
dc.identifier.scopusauthoridTsang, PCK=7102404070en_HK
dc.identifier.scopusauthoridYip, AMW=19838748300en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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