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Article: Promoter methylation of death-associated protein kinase and its role in irradiation response in cervical cancer

TitlePromoter methylation of death-associated protein kinase and its role in irradiation response in cervical cancer
Authors
KeywordsCervical cancer
Death- associated protein kinase
Methylation
Issue Date2008
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htm
Citation
Oncology Reports, 2008, v. 19 n. 5, p. 1339-1345 How to Cite?
AbstractThis study was aimed at investigating the death-associated protein kinase (DAPK) promoter methylation and its clinical relevance in cervical cancer. The DAPK promoter methylation was detected by methylation-specific PCR (MSP) and correlated with DAPK mRNA and protein expression. The effect of DAPK expression on the radiosensitivity of the cervical cancer cell line was assessed by overexpressing DAPK in the radioresistant cell line SiHa. DAPK hypermethylation was found in 56.08% of the cervical cancer samples and was associated with the tumor histological cell type of squamous cell carcinoma (p=0.002) and advanced tumor stage (p=0.005). Subsequently, DAPK protein expression was found to significantly decrease in cervical cancer samples when compared to normal tissues. The DAPK mRNA and protein expression levels were absent or remarkably reduced in SiHa and HeLa in which the DAPK promoter was hypermethylated. The expression levels of DAPK could be restored after demethylation treatment with 5-aza-2′-deoxycytidine. Overexpressing DAPK in vitro had no significant influence to the survival of the radioresistant SiHa cell after being challenged by irradiation. Our findings suggest that DAPK might not directly be responsible for the cellular radio-sensitivity, however, DAPK hypermethylation appeared to be of prognostic significance in the advanced stages of cervical cancer.
Persistent Identifierhttp://hdl.handle.net/10722/87207
ISSN
2015 Impact Factor: 2.486
2015 SCImago Journal Rankings: 0.968
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, RCYen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorTam, KFen_HK
dc.contributor.authorChan, KLen_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:26:42Z-
dc.date.available2010-09-06T09:26:42Z-
dc.date.issued2008en_HK
dc.identifier.citationOncology Reports, 2008, v. 19 n. 5, p. 1339-1345en_HK
dc.identifier.issn1021-335Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87207-
dc.description.abstractThis study was aimed at investigating the death-associated protein kinase (DAPK) promoter methylation and its clinical relevance in cervical cancer. The DAPK promoter methylation was detected by methylation-specific PCR (MSP) and correlated with DAPK mRNA and protein expression. The effect of DAPK expression on the radiosensitivity of the cervical cancer cell line was assessed by overexpressing DAPK in the radioresistant cell line SiHa. DAPK hypermethylation was found in 56.08% of the cervical cancer samples and was associated with the tumor histological cell type of squamous cell carcinoma (p=0.002) and advanced tumor stage (p=0.005). Subsequently, DAPK protein expression was found to significantly decrease in cervical cancer samples when compared to normal tissues. The DAPK mRNA and protein expression levels were absent or remarkably reduced in SiHa and HeLa in which the DAPK promoter was hypermethylated. The expression levels of DAPK could be restored after demethylation treatment with 5-aza-2′-deoxycytidine. Overexpressing DAPK in vitro had no significant influence to the survival of the radioresistant SiHa cell after being challenged by irradiation. Our findings suggest that DAPK might not directly be responsible for the cellular radio-sensitivity, however, DAPK hypermethylation appeared to be of prognostic significance in the advanced stages of cervical cancer.en_HK
dc.languageengen_HK
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htmen_HK
dc.relation.ispartofOncology Reportsen_HK
dc.subjectCervical canceren_HK
dc.subjectDeath- associated protein kinaseen_HK
dc.subjectMethylationen_HK
dc.titlePromoter methylation of death-associated protein kinase and its role in irradiation response in cervical canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1021-335X&volume=19&spage=1339&epage=1345&date=2008&atitle=Promoter+methylation+of+death-associated+protein+kinase+and+its+role+in+irradiation+response+in+cervical+canceren_HK
dc.identifier.emailLiu, SS: stephasl@hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailChan, KL: kklchan@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityChan, KL=rp00499en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-47549085777en_HK
dc.identifier.hkuros145609en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47549085777&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1339en_HK
dc.identifier.epage1345en_HK
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridLeung, RCY=7101876103en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridTam, KF=7201692816en_HK
dc.identifier.scopusauthoridChan, KL=8655666700en_HK
dc.identifier.scopusauthoridWong, LC=55436382200en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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