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Article: Potential application of the ATP cell viability assay in the measurement of intrinsic radiosensitivity in cervical cancer

TitlePotential application of the ATP cell viability assay in the measurement of intrinsic radiosensitivity in cervical cancer
Authors
KeywordsATP cell viability assay
Cervical cancer
Radiosensitivity
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2005, v. 96 n. 3, p. 765-770 How to Cite?
AbstractBackground. Intrinsic radiosensitivity using the clonogenic assay and the cell surviving fraction at 2 Gy (SF 2) has been shown to be an independent prognostic factor for patient response to radiotherapy in carcinoma of the cervix. The clonogenic assay has significant shortcomings, making it unsuitable for routine clinical use. The ATP cell viability assay (ATP-CVA) has been shown to have a high tumor evaluability rate, technical simplicity, and reproducibility in chemosensitivity testing. Aims. This study compares the ATP-CVA with the clonogenic assay in the in vitro radiosensitivity testing of cervical cancer cell lines. Correlation of in vitro radiosensitivity and in vivo patient response was also determined. Methods. Five cervical carcinoma cell lines (SiHa, HeLa, Caski, C-33A, and C4-1) were tested using the ATP-CVA and the clonogenic assay. Survival curves were plotted and the mean SF 2 values obtained by the two different assay methods were compared using ANOVA to see if there were significant differences. Mean SF 2 values obtained from 27 cervical cancers were compared with clinical outcomes. Results. The SF 2 values for the cell lines ranged from 0.28 to 0.67 when tested using the ATP-CVA. Using the clonogenic assay, the SF 2 values ranged from 0.27 to 0.70. ANOVA with Bonferroni pairwise multiple comparison showed no significant difference between the mean SF 2 values for the individual cell lines between the two assay methods. Twenty-three cervical cancer samples (85%) were evaluable for SF 2 using ATP-CVA. The mean SF 2 values of patients who had locoregional failure were significantly higher than those who achieved local control (P < 0.01). Conclusions. Testing intrinsic radiosensitivity using the surviving fraction at 2 Gy (SF 2) is comparable using the two assay methods of ATP-CVA and clonogenic assay. The ATP-CVA should be further investigated in the testing of intrinsic radiosensitivity in patients with cervical cancer. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87165
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTam, KFen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorTsang, PCKen_HK
dc.contributor.authorKwong, PWKen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:26:10Z-
dc.date.available2010-09-06T09:26:10Z-
dc.date.issued2005en_HK
dc.identifier.citationGynecologic Oncology, 2005, v. 96 n. 3, p. 765-770en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87165-
dc.description.abstractBackground. Intrinsic radiosensitivity using the clonogenic assay and the cell surviving fraction at 2 Gy (SF 2) has been shown to be an independent prognostic factor for patient response to radiotherapy in carcinoma of the cervix. The clonogenic assay has significant shortcomings, making it unsuitable for routine clinical use. The ATP cell viability assay (ATP-CVA) has been shown to have a high tumor evaluability rate, technical simplicity, and reproducibility in chemosensitivity testing. Aims. This study compares the ATP-CVA with the clonogenic assay in the in vitro radiosensitivity testing of cervical cancer cell lines. Correlation of in vitro radiosensitivity and in vivo patient response was also determined. Methods. Five cervical carcinoma cell lines (SiHa, HeLa, Caski, C-33A, and C4-1) were tested using the ATP-CVA and the clonogenic assay. Survival curves were plotted and the mean SF 2 values obtained by the two different assay methods were compared using ANOVA to see if there were significant differences. Mean SF 2 values obtained from 27 cervical cancers were compared with clinical outcomes. Results. The SF 2 values for the cell lines ranged from 0.28 to 0.67 when tested using the ATP-CVA. Using the clonogenic assay, the SF 2 values ranged from 0.27 to 0.70. ANOVA with Bonferroni pairwise multiple comparison showed no significant difference between the mean SF 2 values for the individual cell lines between the two assay methods. Twenty-three cervical cancer samples (85%) were evaluable for SF 2 using ATP-CVA. The mean SF 2 values of patients who had locoregional failure were significantly higher than those who achieved local control (P < 0.01). Conclusions. Testing intrinsic radiosensitivity using the surviving fraction at 2 Gy (SF 2) is comparable using the two assay methods of ATP-CVA and clonogenic assay. The ATP-CVA should be further investigated in the testing of intrinsic radiosensitivity in patients with cervical cancer. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subjectATP cell viability assayen_HK
dc.subjectCervical canceren_HK
dc.subjectRadiosensitivityen_HK
dc.titlePotential application of the ATP cell viability assay in the measurement of intrinsic radiosensitivity in cervical canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=96&issue=3&spage=765&epage=770&date=2005&atitle=Potential+application+of+the+ATP+cell+viability+assay+in+the+measurement+of+intrinsic+radiosensitivity+in+cervical+canceren_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygyno.2004.11.025en_HK
dc.identifier.pmid15721424-
dc.identifier.scopuseid_2-s2.0-13844281564en_HK
dc.identifier.hkuros101903en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13844281564&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume96en_HK
dc.identifier.issue3en_HK
dc.identifier.spage765en_HK
dc.identifier.epage770en_HK
dc.identifier.isiWOS:000227615600029-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTam, KF=35622901400en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridTsang, PCK=7102404070en_HK
dc.identifier.scopusauthoridKwong, PWK=7006992418en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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