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Article: Abnormal expression of epidermal growth factor receptor and c-erbB2 in squamous cell carcinoma of the cervix: Correlation with human papillomavirus and prognosis

TitleAbnormal expression of epidermal growth factor receptor and c-erbB2 in squamous cell carcinoma of the cervix: Correlation with human papillomavirus and prognosis
Authors
Keywordsc-erbB2
DNA amplification
Epidermal growth factor receptor
Human papillomavirus
Immunohistochemical staining
Ribonuclease protection assay
Issue Date2001
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBI
Citation
Tumor Biology, 2001, v. 22 n. 3, p. 176-183 How to Cite?
AbstractThe aim of this study is to assess the expression of epidermal growth factor receptor (EGFR) and c-erbB2 and their correlation with human papillomavirus (HPV) status and prognosis in squamous cell carcinoma of the cervix. The expression of EGFR and c-erbB2 was studied at the protein level using the immunohistochemical (IHC) staining method, at the RNA level using the ribonuclease protection assay and at the DNA level using Southern blot and hybridization method. One hundred and one patients with squamous cell carcinoma of the cervix were recruited. Fifty-one patients were of stage 1B/2A and 50 patients were of stage 2B and above. Positive IHC stainings of EGFR and c-erbB2 proteins were found in 74.2 and 19.8% of cases, respectively. DNA amplifications of EGFR and c-erbB2 genes were detected in 35.4 and 17.2%, respectively. Of the patients showing positive EGFR and c-erbB2 staining, only 39.2 and 25%, respectively, showed DNA amplifications. RNA overexpression of EGFR or c-erbB2 was only detected in 2% of cervical cancers and was associated with positive staining and DNA amplifications. HPV was detected in 79.2% of the cases by HPV consensus primers L1, in 57.4% for HPV 16 and 27.7% for HPV 18. The abnormal expression of EGFR and c-erbB2 had no correlation with HPV detection and had no prognostic significance on survival. Copyright © 2001 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/87158
ISSN
2015 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.989
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorCheng, DKLen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorWong, LCen_HK
dc.date.accessioned2010-09-06T09:26:04Z-
dc.date.available2010-09-06T09:26:04Z-
dc.date.issued2001en_HK
dc.identifier.citationTumor Biology, 2001, v. 22 n. 3, p. 176-183en_HK
dc.identifier.issn1010-4283en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87158-
dc.description.abstractThe aim of this study is to assess the expression of epidermal growth factor receptor (EGFR) and c-erbB2 and their correlation with human papillomavirus (HPV) status and prognosis in squamous cell carcinoma of the cervix. The expression of EGFR and c-erbB2 was studied at the protein level using the immunohistochemical (IHC) staining method, at the RNA level using the ribonuclease protection assay and at the DNA level using Southern blot and hybridization method. One hundred and one patients with squamous cell carcinoma of the cervix were recruited. Fifty-one patients were of stage 1B/2A and 50 patients were of stage 2B and above. Positive IHC stainings of EGFR and c-erbB2 proteins were found in 74.2 and 19.8% of cases, respectively. DNA amplifications of EGFR and c-erbB2 genes were detected in 35.4 and 17.2%, respectively. Of the patients showing positive EGFR and c-erbB2 staining, only 39.2 and 25%, respectively, showed DNA amplifications. RNA overexpression of EGFR or c-erbB2 was only detected in 2% of cervical cancers and was associated with positive staining and DNA amplifications. HPV was detected in 79.2% of the cases by HPV consensus primers L1, in 57.4% for HPV 16 and 27.7% for HPV 18. The abnormal expression of EGFR and c-erbB2 had no correlation with HPV detection and had no prognostic significance on survival. Copyright © 2001 S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBIen_HK
dc.relation.ispartofTumor Biologyen_HK
dc.subjectc-erbB2en_HK
dc.subjectDNA amplificationen_HK
dc.subjectEpidermal growth factor receptoren_HK
dc.subjectHuman papillomavirusen_HK
dc.subjectImmunohistochemical stainingen_HK
dc.subjectRibonuclease protection assayen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshCarcinoma, Squamous Cell - metabolism - mortality - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Amplificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPapillomaviridae - isolation & purificationen_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshReceptor, Epidermal Growth Factor - metabolismen_HK
dc.subject.meshReceptor, erbB-2 - metabolismen_HK
dc.subject.meshSurvival Analysisen_HK
dc.subject.meshUterine Cervical Neoplasms - metabolism - mortality - virologyen_HK
dc.titleAbnormal expression of epidermal growth factor receptor and c-erbB2 in squamous cell carcinoma of the cervix: Correlation with human papillomavirus and prognosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1010-4283&volume=22&spage=176&epage=183&date=2001&atitle=Abnormal+expression+of+epidermal+growth+factor+receptor+and+c-erbB2+in+squamous+cell+carcinoma+of+the+cervix:+correlation+with+human+papillomavirus+and+prognosisen_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000050613en_HK
dc.identifier.pmid11275796-
dc.identifier.scopuseid_2-s2.0-0035077048en_HK
dc.identifier.hkuros57177en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035077048&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue3en_HK
dc.identifier.spage176en_HK
dc.identifier.epage183en_HK
dc.identifier.isiWOS:000167855500007-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridCheng, DKL=7402806161en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridWong, LC=36947357000en_HK

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