File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Hypermethylation of SOX2 gene in hydatidiform mole and choriocarcinoma

TitleHypermethylation of SOX2 gene in hydatidiform mole and choriocarcinoma
Authors
KeywordsChoriocarcinoma
Hydatidiform mole
Methylation
Placenta
SOX2
Issue Date2008
PublisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
Citation
Reproductive Sciences, 2008, v. 15 n. 7, p. 735-744 How to Cite?
AbstractThis study investigated the expression and methylation profiles of SOX2, a stem cell-related transcription factor, in placentas and gestational trophoblastic disease. The methylation status of SOX2 promoter region in 55 hydatidiform moles, 4 choriocarcinoma, 23 first trimester, and 15 term placentas was evaluated by methylation-specific polymerase chain reaction. The methylated allele was found in 4.4% (1/23) of first trimester placentas, 26.7% (4/15) term placentas, and 56.4% (31/55) of hydatidiform moles and all choriocarcinoma samples and cell lines. A significant reduction in SOX2 messenger RNA expression was found in the hydatidiform moles (P =.027) when compared with that in the placentas. SOX2 messenger RNA expression was significantly correlated with SOX2 hypermethylation (P <.001). SOX2 expression was restored in choriocarcinoma cell lines following treatment to 5-Aza-2′-deoxycytidine and/or Trichostatin A, demethylation and histone deacetylase inhibitors, respectively, and the response was synergistic. Epigenetic mechanisms may play important role on the transcriptional regulation of SOX2 and contribute to pathogenesis of gestational trophoblastic disease. © 2008 by the Society for Gynecologic Investigation.
Persistent Identifierhttp://hdl.handle.net/10722/87094
ISSN
2015 Impact Factor: 2.429
2015 SCImago Journal Rankings: 0.989
PubMed Central ID
References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLi, ASMen_HK
dc.contributor.authorSiu, MKYen_HK
dc.contributor.authorZhang, Hen_HK
dc.contributor.authorWong, ESYen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-09-06T09:25:15Z-
dc.date.available2010-09-06T09:25:15Z-
dc.date.issued2008en_HK
dc.identifier.citationReproductive Sciences, 2008, v. 15 n. 7, p. 735-744en_HK
dc.identifier.issn1933-7191en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87094-
dc.description.abstractThis study investigated the expression and methylation profiles of SOX2, a stem cell-related transcription factor, in placentas and gestational trophoblastic disease. The methylation status of SOX2 promoter region in 55 hydatidiform moles, 4 choriocarcinoma, 23 first trimester, and 15 term placentas was evaluated by methylation-specific polymerase chain reaction. The methylated allele was found in 4.4% (1/23) of first trimester placentas, 26.7% (4/15) term placentas, and 56.4% (31/55) of hydatidiform moles and all choriocarcinoma samples and cell lines. A significant reduction in SOX2 messenger RNA expression was found in the hydatidiform moles (P =.027) when compared with that in the placentas. SOX2 messenger RNA expression was significantly correlated with SOX2 hypermethylation (P <.001). SOX2 expression was restored in choriocarcinoma cell lines following treatment to 5-Aza-2′-deoxycytidine and/or Trichostatin A, demethylation and histone deacetylase inhibitors, respectively, and the response was synergistic. Epigenetic mechanisms may play important role on the transcriptional regulation of SOX2 and contribute to pathogenesis of gestational trophoblastic disease. © 2008 by the Society for Gynecologic Investigation.en_HK
dc.languageengen_HK
dc.publisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.comen_HK
dc.relation.ispartofReproductive Sciencesen_HK
dc.rightsReproductive Sciences. Copyright © Sage Publications, Inc.en_HK
dc.subjectChoriocarcinomaen_HK
dc.subjectHydatidiform moleen_HK
dc.subjectMethylationen_HK
dc.subjectPlacentaen_HK
dc.subjectSOX2en_HK
dc.subject.meshChoriocarcinoma - genetics - metabolism-
dc.subject.meshDNA Methylation-
dc.subject.meshHydatidiform Mole - genetics - metabolism-
dc.subject.meshSOXB1 Transcription Factors - genetics - metabolism-
dc.subject.meshUterine Neoplasms - genetics - metabolism-
dc.titleHypermethylation of SOX2 gene in hydatidiform mole and choriocarcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1933-7191&volume=15&issue=7&spage=735&epage=744&date=2008&atitle=Hypermethylation+of+SOX2+gene+in+hydatidiform+mole+and+choriocarcinomaen_HK
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authoritySiu, MKY=rp00275en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1177/1933719108322433en_HK
dc.identifier.pmid18836133-
dc.identifier.pmcidPMC2929617-
dc.identifier.scopuseid_2-s2.0-52449132308en_HK
dc.identifier.hkuros166974en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-52449132308&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue7en_HK
dc.identifier.spage735en_HK
dc.identifier.epage744en_HK
dc.publisher.placeUnited Statesen_HK
dc.relation.projectStem cell related genes in gestational trophoblastic diseases-
dc.identifier.scopusauthoridLi, ASM=24076332400en_HK
dc.identifier.scopusauthoridSiu, MKY=24924018400en_HK
dc.identifier.scopusauthoridZhang, H=36560843800en_HK
dc.identifier.scopusauthoridWong, ESY=23101622300en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats