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Article: Differential gene expression in cervical cancer cell lines before and after ionizing radiation.

TitleDifferential gene expression in cervical cancer cell lines before and after ionizing radiation.
Authors
Issue Date2003
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2003, v. 22 n. 5, p. 1091-1099 How to Cite?
AbstractOur previous study demonstrated a correlation between increased apoptotic index in pre-radiotherapy cervical cancer and poor patient survival. Apoptosis may thus play an important role in the response of cervical cancer to radiation. Most of cervical carcinomas are associated with human papillomavirus (HPV), and the oncoproteins E6 and E7 disrupt the functions of tumour suppressor genes, resulting in genetic alteration. To understand the multiple genetic changes related to cell radiosensitivity and the induction of apoptosis, two cervical cancer cell lines, SiHa (with HPV infection) and C-33A (contains a mutant p53 gene), were selected for present studied. The gene expression patterns in these cell lines were compared before and after radiation. When compared to normal cervical tissues, differential expressions were observed in 46 genes among the two cell lines studied. Thirty-three genes showed altered expressions after radiation induction. Three out of ten genes that showed differential responsiveness to radiation in the two cell lines were further confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). Bak and c-abl were found to be potential genes that may play important roles in signaling apoptosis in cervical cancer cells following radiation induction.
Persistent Identifierhttp://hdl.handle.net/10722/87062
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorCheung, ANen_HK
dc.contributor.authorNgan, HYen_HK
dc.date.accessioned2010-09-06T09:24:49Z-
dc.date.available2010-09-06T09:24:49Z-
dc.date.issued2003en_HK
dc.identifier.citationInternational Journal Of Oncology, 2003, v. 22 n. 5, p. 1091-1099en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87062-
dc.description.abstractOur previous study demonstrated a correlation between increased apoptotic index in pre-radiotherapy cervical cancer and poor patient survival. Apoptosis may thus play an important role in the response of cervical cancer to radiation. Most of cervical carcinomas are associated with human papillomavirus (HPV), and the oncoproteins E6 and E7 disrupt the functions of tumour suppressor genes, resulting in genetic alteration. To understand the multiple genetic changes related to cell radiosensitivity and the induction of apoptosis, two cervical cancer cell lines, SiHa (with HPV infection) and C-33A (contains a mutant p53 gene), were selected for present studied. The gene expression patterns in these cell lines were compared before and after radiation. When compared to normal cervical tissues, differential expressions were observed in 46 genes among the two cell lines studied. Thirty-three genes showed altered expressions after radiation induction. Three out of ten genes that showed differential responsiveness to radiation in the two cell lines were further confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). Bak and c-abl were found to be potential genes that may play important roles in signaling apoptosis in cervical cancer cells following radiation induction.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational journal of oncologyen_HK
dc.subject.meshEnzymes - genetics-
dc.subject.meshGene Expression Regulation, Neoplastic - radiation effects-
dc.subject.meshNeoplasm Proteins/genetics-
dc.subject.meshDNA Primers-
dc.subject.meshUterine Cervical Neoplasms - genetics - mortality - pathology - radiotherapy-
dc.titleDifferential gene expression in cervical cancer cell lines before and after ionizing radiation.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=22&issue=5&spage=1091&epage=1099&date=2003&atitle=Differential+gene+expression+in+cervical+cancer+cell+lines+before+and+after+ionizing+radiationen_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.emailCheung, AN:anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HY:hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityCheung, AN=rp00542en_HK
dc.identifier.authorityNgan, HY=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12684676-
dc.identifier.scopuseid_2-s2.0-0042128628en_HK
dc.identifier.hkuros83965en_HK
dc.identifier.volume22en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1091en_HK
dc.identifier.epage1099en_HK
dc.identifier.isiWOS:000182165400019-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridCheung, AN=54927484100en_HK
dc.identifier.scopusauthoridNgan, HY=34571944100en_HK

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