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Article: Detection of human papillomavirus DNA in malignant lesions from Chinese women with carcinomas of the upper genital tract

TitleDetection of human papillomavirus DNA in malignant lesions from Chinese women with carcinomas of the upper genital tract
Authors
KeywordsEndometrial and ovarian carcinomas
Human papillomavirus
Polymerase chain reaction
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2002, v. 87 n. 1, p. 104-111 How to Cite?
AbstractObjective. The aim of this study was to determine the prevalence of high-risk oncogenic human papillomaviruses (HPVs) in malignant lesions from Hong Kong Chinese women with carcinomas of the upper genital tract. Methods. The presence of high-risk HPVs in 55 cases of endometrial adenocarcinomas and 60 cases of primary epithelial ovarian cancers was detected by polymerase chain reaction (PCR) using consensus primers complementary to late 1 (L1) gene of the genital HPVs. Amplified PCR products were verified and typed by Southern blot analysis using 32P-labeled DNA probes prepared from cloned HPV-16 and -18 plasmids. To confirm the presence of high-risk HPV types in the tumor tissues, PCR amplification using HPV type 16- and 18-specific primers for part of the E6 gene were also carried out. Results. While HPV-18 was not detected, HPV-16 DNA sequences were identified in 5 (9.1%) of the 55 studied endometrial carcinoma samples. Of the 5 HPV-16-positive cases, there were 4 stage I, and 1 stage II endometrial cancer. In addition, 6 (10%) of the 60 epithelial ovarian carcinomas were positive for high-risk HPVs, which included 5 cases with HPV-16 and 1 case with HPV-18. Clinical staging revealed that 5 of the 6 HPV-positive cases were stage I and the remaining case was stage III ovarian cancer. Histology of the 6 HPV-positive cases showed that there were 1 case of clear-cell adenocarcinoma, 1 case of mucinous cystadenocarcinoma, and 4 cases of mucinous tumor of borderline malignancy. No other HPV types were detected. Conclusion. High-risk HPV was detected in ∼10% of the tumor samples from women with upper genital tract carcinomas. As compared to the high positive rate of HPV infections in cervical cancer, it appears that HPV infection plays a relatively minor role in the pathogenesis of endometrial and ovarian carcinomas. © 2002 Elsevier Science (USA).
Persistent Identifierhttp://hdl.handle.net/10722/87040
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorIp, SMen_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorXu, CMen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorTsang, PCKen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:24:32Z-
dc.date.available2010-09-06T09:24:32Z-
dc.date.issued2002en_HK
dc.identifier.citationGynecologic Oncology, 2002, v. 87 n. 1, p. 104-111en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87040-
dc.description.abstractObjective. The aim of this study was to determine the prevalence of high-risk oncogenic human papillomaviruses (HPVs) in malignant lesions from Hong Kong Chinese women with carcinomas of the upper genital tract. Methods. The presence of high-risk HPVs in 55 cases of endometrial adenocarcinomas and 60 cases of primary epithelial ovarian cancers was detected by polymerase chain reaction (PCR) using consensus primers complementary to late 1 (L1) gene of the genital HPVs. Amplified PCR products were verified and typed by Southern blot analysis using 32P-labeled DNA probes prepared from cloned HPV-16 and -18 plasmids. To confirm the presence of high-risk HPV types in the tumor tissues, PCR amplification using HPV type 16- and 18-specific primers for part of the E6 gene were also carried out. Results. While HPV-18 was not detected, HPV-16 DNA sequences were identified in 5 (9.1%) of the 55 studied endometrial carcinoma samples. Of the 5 HPV-16-positive cases, there were 4 stage I, and 1 stage II endometrial cancer. In addition, 6 (10%) of the 60 epithelial ovarian carcinomas were positive for high-risk HPVs, which included 5 cases with HPV-16 and 1 case with HPV-18. Clinical staging revealed that 5 of the 6 HPV-positive cases were stage I and the remaining case was stage III ovarian cancer. Histology of the 6 HPV-positive cases showed that there were 1 case of clear-cell adenocarcinoma, 1 case of mucinous cystadenocarcinoma, and 4 cases of mucinous tumor of borderline malignancy. No other HPV types were detected. Conclusion. High-risk HPV was detected in ∼10% of the tumor samples from women with upper genital tract carcinomas. As compared to the high positive rate of HPV infections in cervical cancer, it appears that HPV infection plays a relatively minor role in the pathogenesis of endometrial and ovarian carcinomas. © 2002 Elsevier Science (USA).en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subjectEndometrial and ovarian carcinomasen_HK
dc.subjectHuman papillomavirusen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subject.meshAdenocarcinoma - virologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshCapsid Proteinsen_HK
dc.subject.meshConsensus Sequenceen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshDNA, Viral - analysis - geneticsen_HK
dc.subject.meshDNA-Binding Proteinsen_HK
dc.subject.meshEndometrial Neoplasms - virologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOncogene Proteins, Viral - geneticsen_HK
dc.subject.meshOvarian Neoplasms - virologyen_HK
dc.subject.meshPapillomaviridae - geneticsen_HK
dc.subject.meshPapillomavirus Infections - complications - virologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRepressor Proteinsen_HK
dc.subject.meshTumor Virus Infections - complications - virologyen_HK
dc.titleDetection of human papillomavirus DNA in malignant lesions from Chinese women with carcinomas of the upper genital tracten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=87&issue=1&spage=104&epage=111&date=2002&atitle=Detection+of+human+papillomavirus+DNA+in+Malignant+lesions+from+Chinese+women+with+carcinomas+of+the+upper+genital+tracten_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/gyno.2002.6784en_HK
dc.identifier.pmid12468350-
dc.identifier.scopuseid_2-s2.0-0036924679en_HK
dc.identifier.hkuros78600en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036924679&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue1en_HK
dc.identifier.spage104en_HK
dc.identifier.epage111en_HK
dc.identifier.isiWOS:000178688700016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridIp, SM=55041321600en_HK
dc.identifier.scopusauthoridWong, LC=7402092003en_HK
dc.identifier.scopusauthoridXu, CM=7404179693en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridTsang, PCK=7102404070en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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