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Article: Apoptosis in gestational trophoblastic disease is correlated with clinical outcome and Bcl-2 expression but not Bax expression

TitleApoptosis in gestational trophoblastic disease is correlated with clinical outcome and Bcl-2 expression but not Bax expression
Authors
KeywordsApoptosis
Bar
Bcl-2
Gestational trophoblastic disease
Issue Date1999
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
Citation
Modern Pathology, 1999, v. 12 n. 11, p. 1025-1033 How to Cite?
AbstractApoptosis has been found to play a crucial role in the pathogenesis and prognosis of many human diseases. The pathogenesis of gestational trophoblastic disease (GTD), which encompasses hydatidiform moles (HMs) and choriocarcinomas (CCAs), is not fully understood. Prognostic indicators of HM have also been scanty. In this study, we investigated apoptotic activity and the expression of two apoptosis regulatory genes, Bcl-2 and Bax, in an attempt to determine the role of apoptosis in GTD. Formalin-fixed paraffin- embedded tissue of 33 normal placentas, 14 spontaneous abortions, 14 partial moles, 34 complete moles, and eight CCAs were examined. Apoptotic activity was assessed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. Quantitative assessment of apoptotic index (AI) was calculated as a percentage of TUNEL- positive nuclei. Expression of Bcl-2 and Bax were assessed immunohistochemically. Extensive apoptosis was located in syncytiotrophoblasts, cytotrophoblasts, and vinous stromal cells in all HM cases. Apoptosis was detected at a much lower level in spontaneous abortions and normal placentas. Moreover, in normal placentas, TUNEL positive nuclei were exclusively found in syncytiotrophoblasts. AIs were significantly different among various categories of trophoblastic lesions (P < .001) in an ascending order: normal placentas less than spontaneous abortions less than CCAs less than HMs. Furthermore, AIs of those cases that spontaneously regressed was statistically higher than those that developed persistent trophoblastic disease requiring chemotherapy. AIs of trophoblastic lesions in general inversely correlated with Bcl-2 expression (P < .001), but no significant correlation was found between AI and Bax expression (P > .5). We conclude that AI may be a useful prognostic marker for clinical progress of HMs. Bcl-2 expression is probably regulating apoptosis in normal placentas and GTD, whereas Bax expression is not. The difference in AI and Bcl-2 expression between non-molar placentas and HMs offers a potential adjunctive diagnostic tool to distinguish the two entities.
Persistent Identifierhttp://hdl.handle.net/10722/87036
ISSN
2015 Impact Factor: 5.485
2015 SCImago Journal Rankings: 2.803
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, SYYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorChan, CCWen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2010-09-06T09:24:29Z-
dc.date.available2010-09-06T09:24:29Z-
dc.date.issued1999en_HK
dc.identifier.citationModern Pathology, 1999, v. 12 n. 11, p. 1025-1033en_HK
dc.identifier.issn0893-3952en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87036-
dc.description.abstractApoptosis has been found to play a crucial role in the pathogenesis and prognosis of many human diseases. The pathogenesis of gestational trophoblastic disease (GTD), which encompasses hydatidiform moles (HMs) and choriocarcinomas (CCAs), is not fully understood. Prognostic indicators of HM have also been scanty. In this study, we investigated apoptotic activity and the expression of two apoptosis regulatory genes, Bcl-2 and Bax, in an attempt to determine the role of apoptosis in GTD. Formalin-fixed paraffin- embedded tissue of 33 normal placentas, 14 spontaneous abortions, 14 partial moles, 34 complete moles, and eight CCAs were examined. Apoptotic activity was assessed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. Quantitative assessment of apoptotic index (AI) was calculated as a percentage of TUNEL- positive nuclei. Expression of Bcl-2 and Bax were assessed immunohistochemically. Extensive apoptosis was located in syncytiotrophoblasts, cytotrophoblasts, and vinous stromal cells in all HM cases. Apoptosis was detected at a much lower level in spontaneous abortions and normal placentas. Moreover, in normal placentas, TUNEL positive nuclei were exclusively found in syncytiotrophoblasts. AIs were significantly different among various categories of trophoblastic lesions (P < .001) in an ascending order: normal placentas less than spontaneous abortions less than CCAs less than HMs. Furthermore, AIs of those cases that spontaneously regressed was statistically higher than those that developed persistent trophoblastic disease requiring chemotherapy. AIs of trophoblastic lesions in general inversely correlated with Bcl-2 expression (P < .001), but no significant correlation was found between AI and Bax expression (P > .5). We conclude that AI may be a useful prognostic marker for clinical progress of HMs. Bcl-2 expression is probably regulating apoptosis in normal placentas and GTD, whereas Bax expression is not. The difference in AI and Bcl-2 expression between non-molar placentas and HMs offers a potential adjunctive diagnostic tool to distinguish the two entities.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/en_HK
dc.relation.ispartofModern Pathologyen_HK
dc.subjectApoptosisen_HK
dc.subjectBaren_HK
dc.subjectBcl-2en_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subject.meshApoptosis - physiologyen_HK
dc.subject.meshChoriocarcinoma - genetics - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - physiologyen_HK
dc.subject.meshGenes, bcl-2en_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydatidiform Mole - genetics - pathologyen_HK
dc.subject.meshIn Situ Nick-End Labelingen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshProto-Oncogene Proteins - geneticsen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - geneticsen_HK
dc.subject.meshRetrospective Studiesen_HK
dc.subject.meshTreatment Outcomeen_HK
dc.subject.meshTrophoblastic Tumor, Placental Site - genetics - pathologyen_HK
dc.subject.meshUterine Neoplasms - genetics - pathologyen_HK
dc.subject.meshbcl-2-Associated X Proteinen_HK
dc.titleApoptosis in gestational trophoblastic disease is correlated with clinical outcome and Bcl-2 expression but not Bax expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0893-3952&volume=12&issue=11&spage=1025&epage=1033&date=1999&atitle=Apoptosis+in+gestational+trophoblastic+disease+is+correlated+with+clinical+outcome+and+Bcl-2+expression+but+not+Bax+expressionen_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY:anycheun@hkucc.hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10574599-
dc.identifier.scopuseid_2-s2.0-0032708058en_HK
dc.identifier.hkuros48332en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032708058&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1025en_HK
dc.identifier.epage1033en_HK
dc.identifier.isiWOS:000083821200005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, SYY=7404589620en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridChan, CCW=26643394500en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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