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Article: A comparative study of raw and prepared semen samples from two consecutive days

TitleA comparative study of raw and prepared semen samples from two consecutive days
Authors
Makkar, GHungYu Ng, EShuBiu Yeung, WHo, PC
KeywordsAcrosome reaction
Artificial insemination
Computer-aided sperm analysis
Semen
Issue Date2001
PublisherJournal of Reproductive Medicine, Inc. The Journal's web site is located at http://www.reproductivemedicine.com
Citation
Journal Of Reproductive Medicine For The Obstetrician And Gynecologist, 2001, v. 46 n. 6, p. 565-572 How to Cite?
AbstractOBJECTIVE: To study and compare raw and prepared semen samples from two consecutive days by conventional sperm parameters, various motility characteristics of spermatozoa determined by computer-aided sperm analysis and calcium ionophore-induced acrosome reaction. STUDY DESIGN: Semen samples of male partners in couples undergoing 81 cycles of double intrauterine insemination were studied. The first sample was produced after abstinence of 2-7 days and the second, 24 hours after the first. Both samples were processed by isolate sperm separation medium. RESULTS: Semen volume, sperm concentration and total motile spermatozoa were significantly reduced in day 2 raw and prepared samples, whereas normal morphology, motility characteristics and percentage of acrosome-reacted spermatozoa increased significantly in day 2 inseminated samples as compared to day 1. Oligospermic, asthenospermic and teratozoospermic samples showed a significant improvement in concentration, various motility characteristics and normal morphology of spermatozoa in day 2 samples as compared to day 1. CONCLUSION: Men with normal samples showed improvements in normal morphology and acrosome-reacted spermatozoa, whereas those with subnormal semen samples from day 1 showed a significant improvement in concentration, various spermatozoal velocities and normal morphology on day 2.
Persistent Identifierhttp://hdl.handle.net/10722/87035
ISSN
0024-7758
2022 Impact Factor: 0.2
2023 SCImago Journal Rankings: 0.103
ISI Accession Number ID
WOS:000169584700004
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorMakkar, Gen_HK
dc.contributor.authorHungYu Ng, Een_HK
dc.contributor.authorShuBiu Yeung, Wen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2010-09-06T09:24:28Z-
dc.date.available2010-09-06T09:24:28Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Reproductive Medicine For The Obstetrician And Gynecologist, 2001, v. 46 n. 6, p. 565-572en_HK
dc.identifier.issn0024-7758en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87035-
dc.description.abstractOBJECTIVE: To study and compare raw and prepared semen samples from two consecutive days by conventional sperm parameters, various motility characteristics of spermatozoa determined by computer-aided sperm analysis and calcium ionophore-induced acrosome reaction. STUDY DESIGN: Semen samples of male partners in couples undergoing 81 cycles of double intrauterine insemination were studied. The first sample was produced after abstinence of 2-7 days and the second, 24 hours after the first. Both samples were processed by isolate sperm separation medium. RESULTS: Semen volume, sperm concentration and total motile spermatozoa were significantly reduced in day 2 raw and prepared samples, whereas normal morphology, motility characteristics and percentage of acrosome-reacted spermatozoa increased significantly in day 2 inseminated samples as compared to day 1. Oligospermic, asthenospermic and teratozoospermic samples showed a significant improvement in concentration, various motility characteristics and normal morphology of spermatozoa in day 2 samples as compared to day 1. CONCLUSION: Men with normal samples showed improvements in normal morphology and acrosome-reacted spermatozoa, whereas those with subnormal semen samples from day 1 showed a significant improvement in concentration, various spermatozoal velocities and normal morphology on day 2.en_HK
dc.languageengen_HK
dc.publisherJournal of Reproductive Medicine, Inc. The Journal's web site is located at http://www.reproductivemedicine.comen_HK
dc.relation.ispartofJournal of Reproductive Medicine for the Obstetrician and Gynecologisten_HK
dc.subjectAcrosome reaction-
dc.subjectArtificial insemination-
dc.subjectComputer-aided sperm analysis-
dc.subjectSemen-
dc.subject.meshAcrosome Reactionen_HK
dc.subject.meshAdulten_HK
dc.subject.meshDiagnosis, Computer-Assisteden_HK
dc.subject.meshHumansen_HK
dc.subject.meshInsemination, Artificialen_HK
dc.subject.meshMaleen_HK
dc.subject.meshSemen - physiologyen_HK
dc.subject.meshSexual Abstinenceen_HK
dc.subject.meshSpecimen Handlingen_HK
dc.subject.meshSperm Motilityen_HK
dc.subject.meshTime Factorsen_HK
dc.titleA comparative study of raw and prepared semen samples from two consecutive daysen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1004-3845&volume=46&issue=6&spage=565&epage=572&date=2001&atitle=A+comparative+study+of+raw+and+prepared+semen+samples+from+two+consecutive+daysen_HK
dc.identifier.emailHungYu Ng, E:nghye@hkucc.hku.hken_HK
dc.identifier.emailShuBiu Yeung, W:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityHungYu Ng, E=rp00426en_HK
dc.identifier.authorityShuBiu Yeung, W=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11441681-
dc.identifier.scopuseid_2-s2.0-0034951897en_HK
dc.identifier.hkuros65361en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034951897&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue6en_HK
dc.identifier.spage565en_HK
dc.identifier.epage572en_HK
dc.identifier.isiWOS:000169584700004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMakkar, G=6603413197en_HK
dc.identifier.scopusauthoridHungYu Ng, E=35238184300en_HK
dc.identifier.scopusauthoridShuBiu Yeung, W=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.issnl0024-7758-

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