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Article: p73 Expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy

Titlep73 Expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapy
Authors
Issue Date2004
PublisherAmerican Association for Cancer Research.
Citation
Clinical Cancer Research, 2004, v. 10 n. 10, p. 3309-3316 How to Cite?
AbstractApoptosis is one of the causes of cell death in cervical cancer following radiotherapy (S. S. Liu et al., Eur. J. Cancer, 37: 1104-1110, 2001). By studying the gene expression profile with cDNA apoptotic array, the p73 gene was found overexpressed in radiosensitive cervical cancers when compared with radioresistant ones. To investigate the role of the p73 gene in relation to clinical assessment of radiosensitivity in cervical cancer based on the findings of residual tumor cells in cervical biopsies after completion of radiotherapy, we studied the protein expression of p73 in 59 cervical cancers after radiotherapy and 68 normal cervices using immunohistochemistry. The expression of p73 was found to be significantly increased in cancer samples and, more importantly, in those samples sensitive to radiotherapy (P < 6.001). The overexpression of p73 actually predicted a better prognosis in cervical cancer patients (P < 0.001). To investigate the possible involvement of p73 downstream genes, the protein expressions of p21 and Bax were studied. The expression of p21, but not Bax, was found to be positively correlated with the expression of p73 (P = 0.001). Furthermore, the epigenetic regulation of p73 expression via DNA methylation was also investigated in 103 cervical cancers and 124 normals. Hypermethylation of p73 gene was observed in 38.8% of cervical cancers, and it was significantly associated with reduced or absent p73 expression (P < 0.001). Reactivation of p73 expression in two cervical cancer cell lines by demethylation treatment supported the role of methylation in the regulation of p73 expression. Our findings suggested that p73 expression was related to the radiosensitivity of cervical cancer cells and may play an important role in the regulation of cellular radiosensitivity.
Persistent Identifierhttp://hdl.handle.net/10722/87032
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorLeung, RCYen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorTam, KFen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:24:26Z-
dc.date.available2010-09-06T09:24:26Z-
dc.date.issued2004en_HK
dc.identifier.citationClinical Cancer Research, 2004, v. 10 n. 10, p. 3309-3316en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87032-
dc.description.abstractApoptosis is one of the causes of cell death in cervical cancer following radiotherapy (S. S. Liu et al., Eur. J. Cancer, 37: 1104-1110, 2001). By studying the gene expression profile with cDNA apoptotic array, the p73 gene was found overexpressed in radiosensitive cervical cancers when compared with radioresistant ones. To investigate the role of the p73 gene in relation to clinical assessment of radiosensitivity in cervical cancer based on the findings of residual tumor cells in cervical biopsies after completion of radiotherapy, we studied the protein expression of p73 in 59 cervical cancers after radiotherapy and 68 normal cervices using immunohistochemistry. The expression of p73 was found to be significantly increased in cancer samples and, more importantly, in those samples sensitive to radiotherapy (P < 6.001). The overexpression of p73 actually predicted a better prognosis in cervical cancer patients (P < 0.001). To investigate the possible involvement of p73 downstream genes, the protein expressions of p21 and Bax were studied. The expression of p21, but not Bax, was found to be positively correlated with the expression of p73 (P = 0.001). Furthermore, the epigenetic regulation of p73 expression via DNA methylation was also investigated in 103 cervical cancers and 124 normals. Hypermethylation of p73 gene was observed in 38.8% of cervical cancers, and it was significantly associated with reduced or absent p73 expression (P < 0.001). Reactivation of p73 expression in two cervical cancer cell lines by demethylation treatment supported the role of methylation in the regulation of p73 expression. Our findings suggested that p73 expression was related to the radiosensitivity of cervical cancer cells and may play an important role in the regulation of cellular radiosensitivity.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.en_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshApoptosisen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCervix Uteri - metabolismen_HK
dc.subject.meshDNA - chemistryen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshDNA Primers - chemistryen_HK
dc.subject.meshDNA, Complementary - metabolismen_HK
dc.subject.meshDNA, Viral - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGenes, p53en_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - metabolismen_HK
dc.subject.meshRNA - metabolismen_HK
dc.subject.meshRadiation Tolerance - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSulfites - chemistryen_HK
dc.subject.meshTemperatureen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshUterine Cervical Neoplasms - metabolism - radiotherapyen_HK
dc.subject.meshbcl-2-Associated X Proteinen_HK
dc.subject.meshrho GTP-Binding Proteins - metabolismen_HK
dc.titlep73 Expression is associated with the cellular radiosensitivity in cervical cancer after radiotherapyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=10&spage=3309&epage=3316&date=2004&atitle=p73+expression+is+associated+with+the+cellular+radiosensitivity+in+cervical+cancer+after+radiotherapyen_HK
dc.identifier.emailLiu, SS: stephasl@hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/1078-0432.CCR-03-0119en_HK
dc.identifier.pmid15161684-
dc.identifier.scopuseid_2-s2.0-2542433039en_HK
dc.identifier.hkuros86766en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2542433039&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3309en_HK
dc.identifier.epage3316en_HK
dc.identifier.isiWOS:000221626600010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridLeung, RCY=7101876103en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridTam, KF=7201692816en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridWong, LC=55436382200en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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