File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Comparative study of the biological activity of spermatozoa-zona pellucida binding inhibitory factors from human follicular fluid on various sperm function parameters

TitleComparative study of the biological activity of spermatozoa-zona pellucida binding inhibitory factors from human follicular fluid on various sperm function parameters
Authors
KeywordsAcrosome reaction
Female reproductive tract
Fertilization
Follicle
Sperm
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2002, v. 61 n. 2, p. 205-212 How to Cite?
AbstractPrevious studies showed that human follicular fluid (hFF) from gonadotrophin stimulated cycles contained two glycoproteins, named as ZIF-1 and ZIF-2, that reduced the zona binding capacity of spermatozoa. The present study showed that the spermatozoa-zona pellucida binding inhibitory activity was also present in hFF from natural cycle. Using the hemizona binding assay, the inhibitory effect of ZIF-1 on the zona binding capacity of spermatozoa was dose-dependent. The effect of ZIF-2 was also dose-dependent, in the range of 10-100 ng/ml. The inhibitory effects of both ZIF-1 and -2 increased with the duration of the spermatozoa-ZIF interaction. The effect of the former was present up to 120 min incubation, whilst that of latter occurred for the first 90 min. The zona binding inhibitory effect of ZIF-1 and -2 was additive when they were used together to treat the spermatozoa. The biological activity of ZIFs on other sperm parameters that might affect spermatozoa-zona pellucida binding was also investigated. ZIF-1 did not affect the acrosomal status of human spermatozoa while ZIF-2 significantly increased the number of acrosome reacted spermatozoa in the range of 0.1-10 μg. However, the increase in the incidence of acrosome-reacted spermatozoa after ZIF-2 treatment could not totally account the inhibitory effect of ZIF-2 on zona binding. Both glycoproteins did not affect the motility of human spermatozoa. Radioactively-labelled ZIFs bound to human spermatozoa. Unlabelled ZIF displaced the bound radioactivity of spermatozoa treated with the corresponding labelled ZIF. These suggested the presence of specific binding sites of ZIFs on human spermatozoa. © 2002 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/87029
ISSN
2015 Impact Factor: 2.141
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:24:23Z-
dc.date.available2010-09-06T09:24:23Z-
dc.date.issued2002en_HK
dc.identifier.citationMolecular Reproduction And Development, 2002, v. 61 n. 2, p. 205-212en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87029-
dc.description.abstractPrevious studies showed that human follicular fluid (hFF) from gonadotrophin stimulated cycles contained two glycoproteins, named as ZIF-1 and ZIF-2, that reduced the zona binding capacity of spermatozoa. The present study showed that the spermatozoa-zona pellucida binding inhibitory activity was also present in hFF from natural cycle. Using the hemizona binding assay, the inhibitory effect of ZIF-1 on the zona binding capacity of spermatozoa was dose-dependent. The effect of ZIF-2 was also dose-dependent, in the range of 10-100 ng/ml. The inhibitory effects of both ZIF-1 and -2 increased with the duration of the spermatozoa-ZIF interaction. The effect of the former was present up to 120 min incubation, whilst that of latter occurred for the first 90 min. The zona binding inhibitory effect of ZIF-1 and -2 was additive when they were used together to treat the spermatozoa. The biological activity of ZIFs on other sperm parameters that might affect spermatozoa-zona pellucida binding was also investigated. ZIF-1 did not affect the acrosomal status of human spermatozoa while ZIF-2 significantly increased the number of acrosome reacted spermatozoa in the range of 0.1-10 μg. However, the increase in the incidence of acrosome-reacted spermatozoa after ZIF-2 treatment could not totally account the inhibitory effect of ZIF-2 on zona binding. Both glycoproteins did not affect the motility of human spermatozoa. Radioactively-labelled ZIFs bound to human spermatozoa. Unlabelled ZIF displaced the bound radioactivity of spermatozoa treated with the corresponding labelled ZIF. These suggested the presence of specific binding sites of ZIFs on human spermatozoa. © 2002 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.rightsMolecular Reproduction And Development. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectAcrosome reactionen_HK
dc.subjectFemale reproductive tracten_HK
dc.subjectFertilizationen_HK
dc.subjectFollicleen_HK
dc.subjectSpermen_HK
dc.titleComparative study of the biological activity of spermatozoa-zona pellucida binding inhibitory factors from human follicular fluid on various sperm function parametersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1040-452X&volume=61&spage=205&epage=212&date=2002&atitle=Comparative+study+of+the+biological+activity+of+spermatozoa-zona+pellucida+binding+inhibitory+factors+from+human+follicular+fluid+on+various+sperm+function+parametersen_HK
dc.identifier.emailChiu, PCN:pchiucn@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrd.1149en_HK
dc.identifier.pmid11803556-
dc.identifier.scopuseid_2-s2.0-0036137510en_HK
dc.identifier.hkuros65425en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036137510&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue2en_HK
dc.identifier.spage205en_HK
dc.identifier.epage212en_HK
dc.identifier.isiWOS:000173100800010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats