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Article: Distribution of six oncogenic types of human papillomavirus and type 16 integration analysis in Chinese women with cervical precancerous lesions and carcinomas

TitleDistribution of six oncogenic types of human papillomavirus and type 16 integration analysis in Chinese women with cervical precancerous lesions and carcinomas
Authors
KeywordsCervical cancer
Human papillomavirus
Prevalence
Squamous intraepithelial lesion
Viral DNA integration
Issue Date2008
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBI
Citation
Tumor Biology, 2008, v. 29 n. 2, p. 105-113 How to Cite?
AbstractObjectives: The prevalent genotypes of human papillomavirus (HPV) infection and the significance of HPV16 integration in cervical precancerous lesions and cancer of Chinese women were investigated. Methods: HPV genotyping and HPV16 integration status were studied on 100 normal cervical cytology, 90 low-grade (LSIL) and 99 high-grade squamous intraepithelial lesions (HSIL), as well as 96 cervical cancer biopsies using DNA sequencing and quantitative real-time PCR. Results: HPV were detected in 12.0% of normal cytology, 93.3% of LSIL, 90.9% of HSIL and 89.6% of cervical cancer samples. High-risk HPV16, 18, 58, 52, 33 and 31 were the 6 major HPV types observed, composing 45.6, 82.8 and 85.4% of HPV infections in LSIL, HSIL and cervical cancers, respectively, with a significant rising trend in increasing disease severity (p = 0.002). While HPV16 and HPV18 were most commonly found in cervical cancer, HPV58 was prevalently observed in cervical precancerous lesions, and its frequency decreased with increased disease severity. Among HPV16-positive patients, 40.0% of LSIL, 88.9% of HSIL and 86.4% of cancer cases had HPV16 integration. The frequency of the integrated form of HPV16 significantly increased with the severity of the disease. Conclusions: Our results demonstrated that high-risk HPV infection and viral DNA integration were in progressive frequency from low-grade cervical precancerous lesions to cancer. HPV16 integration status had the potential to be a marker for cancer risk assessment of cervical precancerous lesions. Copyright © 2008 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/87022
ISSN
2015 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.989
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorTsang, PCKen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorChan, KKLen_HK
dc.contributor.authorLeung, RCYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:24:17Z-
dc.date.available2010-09-06T09:24:17Z-
dc.date.issued2008en_HK
dc.identifier.citationTumor Biology, 2008, v. 29 n. 2, p. 105-113en_HK
dc.identifier.issn1010-4283en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87022-
dc.description.abstractObjectives: The prevalent genotypes of human papillomavirus (HPV) infection and the significance of HPV16 integration in cervical precancerous lesions and cancer of Chinese women were investigated. Methods: HPV genotyping and HPV16 integration status were studied on 100 normal cervical cytology, 90 low-grade (LSIL) and 99 high-grade squamous intraepithelial lesions (HSIL), as well as 96 cervical cancer biopsies using DNA sequencing and quantitative real-time PCR. Results: HPV were detected in 12.0% of normal cytology, 93.3% of LSIL, 90.9% of HSIL and 89.6% of cervical cancer samples. High-risk HPV16, 18, 58, 52, 33 and 31 were the 6 major HPV types observed, composing 45.6, 82.8 and 85.4% of HPV infections in LSIL, HSIL and cervical cancers, respectively, with a significant rising trend in increasing disease severity (p = 0.002). While HPV16 and HPV18 were most commonly found in cervical cancer, HPV58 was prevalently observed in cervical precancerous lesions, and its frequency decreased with increased disease severity. Among HPV16-positive patients, 40.0% of LSIL, 88.9% of HSIL and 86.4% of cancer cases had HPV16 integration. The frequency of the integrated form of HPV16 significantly increased with the severity of the disease. Conclusions: Our results demonstrated that high-risk HPV infection and viral DNA integration were in progressive frequency from low-grade cervical precancerous lesions to cancer. HPV16 integration status had the potential to be a marker for cancer risk assessment of cervical precancerous lesions. Copyright © 2008 S. Karger AG.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBIen_HK
dc.relation.ispartofTumor Biologyen_HK
dc.subjectCervical canceren_HK
dc.subjectHuman papillomavirusen_HK
dc.subjectPrevalenceen_HK
dc.subjectSquamous intraepithelial lesionen_HK
dc.subjectViral DNA integrationen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshCarcinoma, Squamous Cell - ethnology - genetics - virologyen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshCervical Intraepithelial Neoplasia - ethnology - genetics - virologyen_HK
dc.subject.meshCervix Uteri - virologyen_HK
dc.subject.meshChinaen_HK
dc.subject.meshDNA, Viral - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenes, Viral - geneticsen_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHuman papillomavirus 16 - geneticsen_HK
dc.subject.meshHuman papillomavirus 18 - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOncogenes - geneticsen_HK
dc.subject.meshPapillomavirus Infections - ethnology - geneticsen_HK
dc.subject.meshPrecancerous Conditions - ethnology - genetics - virologyen_HK
dc.subject.meshPrevalenceen_HK
dc.subject.meshTumor Markers, Biological - geneticsen_HK
dc.subject.meshUterine Cervical Neoplasms - ethnology - genetics - virologyen_HK
dc.subject.meshVirus Integration - geneticsen_HK
dc.titleDistribution of six oncogenic types of human papillomavirus and type 16 integration analysis in Chinese women with cervical precancerous lesions and carcinomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1010-4283&volume=29&issue=2&spage=105&epage=113&date=2008&atitle=Distribution+of+Six+Oncogenic+Types+of+Human+Papillomavirus+and+Type+16+Integration+Analysis+in+Chinese+Women+with+Cervical+Precancerous+Lesions+and+Carcinomasen_HK
dc.identifier.emailLiu, SS: stephasl@hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailChan, KKL: kklchan@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityChan, KKL=rp00499en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000135690en_HK
dc.identifier.pmid18515988-
dc.identifier.scopuseid_2-s2.0-47949095648en_HK
dc.identifier.hkuros145292en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47949095648&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue2en_HK
dc.identifier.spage105en_HK
dc.identifier.epage113en_HK
dc.identifier.isiWOS:000257903500005-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridTsang, PCK=7102404070en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridChan, KKL=8655666700en_HK
dc.identifier.scopusauthoridLeung, RCY=7101876103en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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