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Article: E-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours

TitleE-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumours
Authors
KeywordsAntisense oligonucleotide
Cervical cancer
E-cadherin
Methylation
Methyltransferase
MSP
Issue Date2003
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejca
Citation
European Journal Of Cancer, 2003, v. 39 n. 4, p. 517-523 How to Cite?
AbstractA previous study showed E-cadherin expression was lost in some cervical cancer cell lines and tumours. This study was designed to clarify the significance of DNA methylation in silencing E-cadherin expression. We examined promoter methylation of E-cadherin in five cervical cancer cell lines and 20 cervical cancer tissues using methylation-specific PCR (MSP) and bisulphite DNA sequencing. The correlation of E-cadherin methylation and expression together with methyltransferase (DNMT1) were further studied. We found that hypermethylation of E-cadherin was involved in five cervical cancer cell lines and 40% (8/20) of cervical cancer tissues. E-cadherin protein was lost in 6/8 (75%) samples and 3/5 (60%) cell lines with promoter methylation. E-cadherin methylation was significantly correlated with increased DNMT1. Using an antisense DNMT1 oligo to transfect into SiHa HeLa C33A cell line, E-cadherin protein was re-expressed. We concluded that loss of E-cadherin expression was in part correlated with DNA methylation and DNMT1 expression in cervical cancer. © 2003 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87009
ISSN
2015 Impact Factor: 6.163
2015 SCImago Journal Rankings: 3.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, CLen_HK
dc.contributor.authorLiu, SSen_HK
dc.contributor.authorIp, SMen_HK
dc.contributor.authorWong, LCen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:24:07Z-
dc.date.available2010-09-06T09:24:07Z-
dc.date.issued2003en_HK
dc.identifier.citationEuropean Journal Of Cancer, 2003, v. 39 n. 4, p. 517-523en_HK
dc.identifier.issn0959-8049en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87009-
dc.description.abstractA previous study showed E-cadherin expression was lost in some cervical cancer cell lines and tumours. This study was designed to clarify the significance of DNA methylation in silencing E-cadherin expression. We examined promoter methylation of E-cadherin in five cervical cancer cell lines and 20 cervical cancer tissues using methylation-specific PCR (MSP) and bisulphite DNA sequencing. The correlation of E-cadherin methylation and expression together with methyltransferase (DNMT1) were further studied. We found that hypermethylation of E-cadherin was involved in five cervical cancer cell lines and 40% (8/20) of cervical cancer tissues. E-cadherin protein was lost in 6/8 (75%) samples and 3/5 (60%) cell lines with promoter methylation. E-cadherin methylation was significantly correlated with increased DNMT1. Using an antisense DNMT1 oligo to transfect into SiHa HeLa C33A cell line, E-cadherin protein was re-expressed. We concluded that loss of E-cadherin expression was in part correlated with DNA methylation and DNMT1 expression in cervical cancer. © 2003 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/ejcaen_HK
dc.relation.ispartofEuropean Journal of Canceren_HK
dc.subjectAntisense oligonucleotideen_HK
dc.subjectCervical canceren_HK
dc.subjectE-cadherinen_HK
dc.subjectMethylationen_HK
dc.subjectMethyltransferaseen_HK
dc.subjectMSPen_HK
dc.titleE-cadherin expression is silenced by DNA methylation in cervical cancer cell lines and tumoursen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0959-8049&volume=39&issue=4&spage=517&epage=523&date=2003&atitle=E-cadherin+expression+is+silenced+by+DNA+methylation+in+cervical+cancer+cell+lines+and+tumoursen_HK
dc.identifier.emailLiu, SS:stephasl@hku.hken_HK
dc.identifier.emailNgan, HYS:hysngan@hkucc.hku.hken_HK
dc.identifier.authorityLiu, SS=rp00372en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0959-8049(02)00175-2en_HK
dc.identifier.pmid12751384-
dc.identifier.scopuseid_2-s2.0-0037363349en_HK
dc.identifier.hkuros80936en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037363349&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue4en_HK
dc.identifier.spage517en_HK
dc.identifier.epage523en_HK
dc.identifier.isiWOS:000181686800026-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, CL=8269766700en_HK
dc.identifier.scopusauthoridLiu, SS=37102450400en_HK
dc.identifier.scopusauthoridIp, SM=55041321600en_HK
dc.identifier.scopusauthoridWong, LC=7402092003en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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