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Article: Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats

TitleTherapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats
Authors
KeywordsFuzheng Huayu Recipe
Hepatic fibrosis
Hepatic stellate cell
PDGF
Radix Salviae Miltiorrhizae
TGF-β1
TIMP-1
Traditional Chinese Medicine
Issue Date2009
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 142-150 How to Cite?
AbstractBackground/aims: Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe. Methods: Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rβ, and transforming growth factor-beta 1 (TGF-β1) were measured by quantitative RT-PCR and western blot. Results: TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rβ, and it also suppressed protein expression of PDGF-Rβ and TGF-β1. Conclusions: TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rβ, and with the suppression of protein expression of PDGF-Rβ and TGF-β1. © 2009 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/83425
ISSN
2015 Impact Factor: 3.055
2015 SCImago Journal Rankings: 1.156
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant CouncilHKU 7679/06M
Cheng Si Yuan (China-International) Hepatitis Research Foundation
Funding Information:

This study was supported by Research Grant Council to Prof. George K.K. Lau (Grant number: HKU 7679/06M) and Cheng Si Yuan (China-International) Hepatitis Research Foundation.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorCheung, KFen_HK
dc.contributor.authorYe, DWen_HK
dc.contributor.authorYang, ZFen_HK
dc.contributor.authorLu, Len_HK
dc.contributor.authorLiu, CHen_HK
dc.contributor.authorWang, XLen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorLiu, Pen_HK
dc.contributor.authorChen, YCen_HK
dc.contributor.authorLau, GKKen_HK
dc.date.accessioned2010-09-06T08:40:53Z-
dc.date.available2010-09-06T08:40:53Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 142-150en_HK
dc.identifier.issn0378-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83425-
dc.description.abstractBackground/aims: Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe. Methods: Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rβ, and transforming growth factor-beta 1 (TGF-β1) were measured by quantitative RT-PCR and western blot. Results: TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rβ, and it also suppressed protein expression of PDGF-Rβ and TGF-β1. Conclusions: TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rβ, and with the suppression of protein expression of PDGF-Rβ and TGF-β1. © 2009 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_HK
dc.relation.ispartofJournal of Ethnopharmacologyen_HK
dc.rightsJournal of Ethnopharmacology. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectFuzheng Huayu Recipeen_HK
dc.subjectHepatic fibrosisen_HK
dc.subjectHepatic stellate cellen_HK
dc.subjectPDGFen_HK
dc.subjectRadix Salviae Miltiorrhizaeen_HK
dc.subjectTGF-β1en_HK
dc.subjectTIMP-1en_HK
dc.subjectTraditional Chinese Medicineen_HK
dc.subject.meshActins - metabolismen_HK
dc.subject.meshAlanine Transaminase - metabolismen_HK
dc.subject.meshAngiospermsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarbon Tetrachlorideen_HK
dc.subject.meshCollagen - metabolismen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshDrug Combinationsen_HK
dc.subject.meshDrugs, Chinese Herbal - pharmacology - therapeutic useen_HK
dc.subject.meshHepatic Stellate Cells - metabolismen_HK
dc.subject.meshLiver - drug effects - metabolism - pathologyen_HK
dc.subject.meshLiver Cirrhosis - chemically induced - metabolism - prevention & controlen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMedicine, Chinese Traditionalen_HK
dc.subject.meshPhytotherapyen_HK
dc.subject.meshPlatelet-Derived Growth Factor - genetics - metabolismen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshTissue Inhibitor of Metalloproteinase-1 - genetics - metabolismen_HK
dc.subject.meshTransforming Growth Factor beta1 - metabolismen_HK
dc.titleTherapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=124&issue=1&spage=142&epage=150&date=2009&atitle=Therapeutic+efficacy+of+Traditional+Chinese+Medicine+319+recipe+on+hepatic+fibrosis+induced+by+carbon+tetrachloride+in+ratsen_HK
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jep.2009.03.005en_HK
dc.identifier.pmid19501992-
dc.identifier.scopuseid_2-s2.0-67349155604en_HK
dc.identifier.hkuros167040en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349155604&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume124en_HK
dc.identifier.issue1en_HK
dc.identifier.spage142en_HK
dc.identifier.epage150en_HK
dc.identifier.isiWOS:000268201200018-
dc.publisher.placeIrelanden_HK
dc.relation.projectRole of upregulation of toll-like receptor 7 and functional polymorphisms within the toll-like receptor 7 gene in inducing sustained disease remission in chronic hepatitis B infection-
dc.identifier.scopusauthoridCheung, KF=8763216400en_HK
dc.identifier.scopusauthoridYe, DW=35277778400en_HK
dc.identifier.scopusauthoridYang, ZF=14018809600en_HK
dc.identifier.scopusauthoridLu, L=7403963552en_HK
dc.identifier.scopusauthoridLiu, CH=36064322500en_HK
dc.identifier.scopusauthoridWang, XL=24478987700en_HK
dc.identifier.scopusauthoridPoon, RTP=7103097223en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridLiu, P=36065603800en_HK
dc.identifier.scopusauthoridChen, YC=35227073300en_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK

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