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Article: The cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferation

TitleThe cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferation
Authors
Keywordsω-3 fatty acids
Cell cycle
Cyclooxygenase-2
Docosahexaenoic acid
Hepatocellular carcinoma
Issue Date2010
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2010, v. 36 n. 4, p. 991-998 How to Cite?
AbstractGiven the reported side effects associated with chemotherapy and surgical resection, dietary intervention with ω-3 polyunsaturated fatty acids (PUFAs) has been postulated to be an alterative way to prevent liver cancer progression and metastasis. We studied the effects of an ω-3 PUFA, docahexaenoic acid (DHA) on COX-2 expression and the cell cycle control machinery that co-ordinately regulate the HCC cells growth. Our data showed that DHA (0-200 μM) retarded proliferation of the human metastatic HCC cell line MHCC97L dose-dependently. In addition, inhibition of cyclin A/Cdk2 interfered with S-phase progression further in agreement with the result of bivariate flow cytometric analysis which indicated that DNA synthesis time (T s) was significantly prolonged by DHA in MHCC97L. The N-myc oncogene, the heat shock proteins Hsp27 and glucose-related protein 78 (GRP78) as well as the antioxidant enzymes superoxide dismutase may play significant roles in the cell cycle control and reduced-proliferation of MHCC97L by DHA. Our data indicated that it is imperative to develop therapeutic strategy with ω-3 PUFA that simultaneously targets COX-2 and other cell cycle regulators in hepatocarcinogenesis. This study provides novel mechanistic insights into the modulation of DHA on human hepatocarcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/83369
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

This study was supported by grants of the Strategic Research Theme oil Cancer by The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorLee, CYKen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorJor, IWYen_HK
dc.contributor.authorWong, LLYen_HK
dc.contributor.authorWan, MLYen_HK
dc.contributor.authorTanUn, KCen_HK
dc.contributor.authorWan, JMFen_HK
dc.date.accessioned2010-09-06T08:40:13Z-
dc.date.available2010-09-06T08:40:13Z-
dc.date.issued2010en_HK
dc.identifier.citationInternational Journal Of Oncology, 2010, v. 36 n. 4, p. 991-998en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/83369-
dc.description.abstractGiven the reported side effects associated with chemotherapy and surgical resection, dietary intervention with ω-3 polyunsaturated fatty acids (PUFAs) has been postulated to be an alterative way to prevent liver cancer progression and metastasis. We studied the effects of an ω-3 PUFA, docahexaenoic acid (DHA) on COX-2 expression and the cell cycle control machinery that co-ordinately regulate the HCC cells growth. Our data showed that DHA (0-200 μM) retarded proliferation of the human metastatic HCC cell line MHCC97L dose-dependently. In addition, inhibition of cyclin A/Cdk2 interfered with S-phase progression further in agreement with the result of bivariate flow cytometric analysis which indicated that DNA synthesis time (T s) was significantly prolonged by DHA in MHCC97L. The N-myc oncogene, the heat shock proteins Hsp27 and glucose-related protein 78 (GRP78) as well as the antioxidant enzymes superoxide dismutase may play significant roles in the cell cycle control and reduced-proliferation of MHCC97L by DHA. Our data indicated that it is imperative to develop therapeutic strategy with ω-3 PUFA that simultaneously targets COX-2 and other cell cycle regulators in hepatocarcinogenesis. This study provides novel mechanistic insights into the modulation of DHA on human hepatocarcinoma.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectω-3 fatty acidsen_HK
dc.subjectCell cycleen_HK
dc.subjectCyclooxygenase-2en_HK
dc.subjectDocosahexaenoic aciden_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subject.meshAntineoplastic Agents - pharmacology-
dc.subject.meshCarcinoma, Hepatocellular - genetics - metabolism - pathology - secondary-
dc.subject.meshCell Cycle - drug effects-
dc.subject.meshDocosahexaenoic Acids - pharmacology-
dc.subject.meshLiver Neoplasms - genetics - metabolism - pathology-
dc.titleThe cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=36&issue=4&spage=991&epage=998&date=2010&atitle=The+cell+cycle+effects+of+docosahexaenoic+acid+on+human+metastatic+hepatocellular+carcinoma+proliferationen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3892/ijo-00000579en_HK
dc.identifier.pmid20198345-
dc.identifier.scopuseid_2-s2.0-77749299218en_HK
dc.identifier.hkuros169121en_HK
dc.identifier.hkuros258396-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77749299218&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue4en_HK
dc.identifier.spage991en_HK
dc.identifier.epage998en_HK
dc.identifier.isiWOS:000275794300030-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridLee, CYK=38162995600en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridJor, IWY=35731710200en_HK
dc.identifier.scopusauthoridWong, LLY=36128985900en_HK
dc.identifier.scopusauthoridWan, MLY=36129068600en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK

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