Article: Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats

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Publisher Website: 10.1073/pnas.0506735102
Scopus: eid_2-s2.0-25444531712
PMID: 16169905

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Title	Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats
Authors	Lau, SKP1 Woo, PCY1 Li, KSM1 Huang, Y1 Tsoi, HW1 Wong, BHL1 Wong, SSY1 Leung, SY1 Chan, KH1 Yuen, KY1
Issue Date	2005
Publisher	National Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation	Proceedings Of The National Academy Of Sciences Of The United States Of America, 2005, v. 102 n. 39, p. 14040-14045 [How to Cite?] DOI: http://dx.doi.org/10.1073/pnas.0506735102
Abstract	Although the finding of severe acute respiratory syndrome coronavirus (SARS-CoV) in caged palm civets from live animal markets in China has provided evidence for interspecies transmission in the genesis of the SARS epidemic, subsequent studies suggested that the civet may have served only as an amplification host for SARS-CoV. In a surveillance study for CoV in noncaged animals from the wild areas of the Hong Kong Special Administration Region, we identified a CoV closely related to SARS-CoV (bat-SARS-CoV) from 23 (39%) of 59 anal swabs of wild Chinese horseshoe bats (Rhinolophus sinicus) by using RT-PCR. Sequencing and analysis of three bat-SARS-CoV genomes from samples collected at different dates showed that bat-SARS-CoV is closely related to SARS-CoV from humans and civets. Phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b CoV, distantly related to known group 2 CoV. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike genes, ORF 3 and ORF 8, which are the regions where most variations also were observed between human and civet SARS-CoV genomes. In addition, the presence of a 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horse-shoe bats by using an enzyme immunoassay. Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals. © 2005 by The National Academy of Sciences of the USA.
ISSN	0027-8424 2011 Impact Factor: 9.681 2011 SCImago Journal Rankings: 1.754
DOI	http://dx.doi.org/10.1073/pnas.0506735102
ISI Accession Number ID	WOS:000232231900059
References	References in Scopus

DC Field	Value
dc.contributor.author	Lau, SKP
dc.contributor.author	Woo, PCY
dc.contributor.author	Li, KSM
dc.contributor.author	Huang, Y
dc.contributor.author	Tsoi, HW
dc.contributor.author	Wong, BHL
dc.contributor.author	Wong, SSY
dc.contributor.author	Leung, SY
dc.contributor.author	Chan, KH
dc.contributor.author	Yuen, KY
dc.date.accessioned	2010-09-06T07:48:19Z
dc.date.available	2010-09-06T07:48:19Z
dc.date.issued	2005
dc.description.abstract	Although the finding of severe acute respiratory syndrome coronavirus (SARS-CoV) in caged palm civets from live animal markets in China has provided evidence for interspecies transmission in the genesis of the SARS epidemic, subsequent studies suggested that the civet may have served only as an amplification host for SARS-CoV. In a surveillance study for CoV in noncaged animals from the wild areas of the Hong Kong Special Administration Region, we identified a CoV closely related to SARS-CoV (bat-SARS-CoV) from 23 (39%) of 59 anal swabs of wild Chinese horseshoe bats (Rhinolophus sinicus) by using RT-PCR. Sequencing and analysis of three bat-SARS-CoV genomes from samples collected at different dates showed that bat-SARS-CoV is closely related to SARS-CoV from humans and civets. Phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b CoV, distantly related to known group 2 CoV. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike genes, ORF 3 and ORF 8, which are the regions where most variations also were observed between human and civet SARS-CoV genomes. In addition, the presence of a 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horse-shoe bats by using an enzyme immunoassay. Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals. © 2005 by The National Academy of Sciences of the USA.
dc.description.nature	Link_to_subscribed_fulltext
dc.identifier.citation	Proceedings Of The National Academy Of Sciences Of The United States Of America, 2005, v. 102 n. 39, p. 14040-14045 [How to Cite?] DOI: http://dx.doi.org/10.1073/pnas.0506735102
dc.identifier.doi	http://dx.doi.org/10.1073/pnas.0506735102
dc.identifier.epage	14045
dc.identifier.hkuros	118584
dc.identifier.isi	WOS:000232231900059
dc.identifier.issn	0027-8424 2011 Impact Factor: 9.681 2011 SCImago Journal Rankings: 1.754
dc.identifier.issue	39
dc.identifier.pmid	16169905
dc.identifier.scopus	eid_2-s2.0-25444531712
dc.identifier.spage	14040
dc.identifier.uri	http://hdl.handle.net/10722/78913
dc.identifier.volume	102
dc.language	eng
dc.publisher	National Academy of Sciences. The Journal's web site is located at http://www.pnas.org
dc.publisher.place	United States
dc.relation.ispartof	Proceedings of the National Academy of Sciences of the United States of America
dc.relation.references	References in Scopus
dc.subject.mesh	Amino Acid Sequence
dc.subject.mesh	Animals
dc.subject.mesh	Base Sequence
dc.subject.mesh	Chiroptera - virology
dc.subject.mesh	Genome, Viral
dc.subject.mesh	Hong Kong
dc.subject.mesh	Molecular Sequence Data
dc.subject.mesh	Phylogeny
dc.subject.mesh	SARS Virus - classification - genetics - isolation & purification
dc.subject.mesh	Sequence Analysis, DNA
dc.subject.mesh	Viral Proteins - blood - genetics
dc.title	Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats
dc.type	Article

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Article: Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats

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