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Article: Real-time quantitative analysis of polyoma BK viremia and viruria in renal allograft recipients

TitleReal-time quantitative analysis of polyoma BK viremia and viruria in renal allograft recipients
Authors
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2002, v. 103 n. 1, p. 51-56 How to Cite?
AbstractPolyoma BK virus (BKV) remains dormant in the urinary tract and circulating leucocytes and becomes reactivated during immunosuppression. BK viruria is prevalent in renal allograft recipients and BK viremia may be related to nephropathy and allograft rejection. How BK viruria and viremia are related in renal allograft patients is undefined. In this study, BKV copies in paired urine and serum samples of renal allograft recipients were measured by a real time quantitative polymerase chain reaction (Q-PCR) to test the hypothesis that their quantitative relationship might help to delineate viral reactivation patterns in these tissues. Urine and plasma samples from 44 renal allograft recipients with stable graft function were collected during outpatient follow-up and the genome copies of BKV were determined by Q-PCR. All patients showed quantifiable viremia and two groups of patients were identified: one group of patients (n=35) showed low viral load (median: 270/ml, range: 108-1000/ml) and the other group (n=9) with high viral load (median: 5×104/ml, range: 2×104-6×104/ml). The corresponding median levels of viruria were 2000 and 900 ml. BK viremia and viruria were not related quantitatively. BK viremia/viruria were also not related to age, immunosuppression, time and source of renal grafts and serum creatinine levels. The absence of a quantitative relationship between BK viremia and viruria may reflect independent BKV reactivation in different tissues during immunosuppression. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78643
ISSN
2015 Impact Factor: 1.508
2015 SCImago Journal Rankings: 0.866
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_HK
dc.contributor.authorChan, Men_HK
dc.contributor.authorTang, SCWen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorKwong, YLen_HK
dc.date.accessioned2010-09-06T07:45:09Z-
dc.date.available2010-09-06T07:45:09Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Virological Methods, 2002, v. 103 n. 1, p. 51-56en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78643-
dc.description.abstractPolyoma BK virus (BKV) remains dormant in the urinary tract and circulating leucocytes and becomes reactivated during immunosuppression. BK viruria is prevalent in renal allograft recipients and BK viremia may be related to nephropathy and allograft rejection. How BK viruria and viremia are related in renal allograft patients is undefined. In this study, BKV copies in paired urine and serum samples of renal allograft recipients were measured by a real time quantitative polymerase chain reaction (Q-PCR) to test the hypothesis that their quantitative relationship might help to delineate viral reactivation patterns in these tissues. Urine and plasma samples from 44 renal allograft recipients with stable graft function were collected during outpatient follow-up and the genome copies of BKV were determined by Q-PCR. All patients showed quantifiable viremia and two groups of patients were identified: one group of patients (n=35) showed low viral load (median: 270/ml, range: 108-1000/ml) and the other group (n=9) with high viral load (median: 5×104/ml, range: 2×104-6×104/ml). The corresponding median levels of viruria were 2000 and 900 ml. BK viremia and viruria were not related quantitatively. BK viremia/viruria were also not related to age, immunosuppression, time and source of renal grafts and serum creatinine levels. The absence of a quantitative relationship between BK viremia and viruria may reflect independent BKV reactivation in different tissues during immunosuppression. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.en_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBK Virus - isolation & purification - physiologyen_HK
dc.subject.meshComputer Systemsen_HK
dc.subject.meshDNA, Viral - analysis - blood - urineen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunosuppression - adverse effectsen_HK
dc.subject.meshKidney Transplantationen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshPolyomavirus Infections - blood - urine - virologyen_HK
dc.subject.meshPostoperative Complications - blood - urine - virologyen_HK
dc.subject.meshTransplantation, Homologousen_HK
dc.subject.meshTumor Virus Infections - blood - urine - virologyen_HK
dc.subject.meshUrine - virologyen_HK
dc.subject.meshViral Loaden_HK
dc.subject.meshViremia - virologyen_HK
dc.subject.meshVirus Activationen_HK
dc.titleReal-time quantitative analysis of polyoma BK viremia and viruria in renal allograft recipientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=103&issue=1&spage=51&epage=6&date=2002&atitle=Real-time+quantitative+analysis+of+polyoma+BK+viremia+and+viruria+in+renal+allograft+recipientsen_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.emailTang, SCW:scwtang@hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.identifier.authorityTang, SCW=rp00480en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0166-0934(01)00447-5en_HK
dc.identifier.pmid11906732-
dc.identifier.scopuseid_2-s2.0-0036127654en_HK
dc.identifier.hkuros78423en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036127654&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue1en_HK
dc.identifier.spage51en_HK
dc.identifier.epage56en_HK
dc.identifier.isiWOS:000175643700005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.scopusauthoridChan, M=36941301500en_HK
dc.identifier.scopusauthoridTang, SCW=7403437082en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK

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