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Article: Establishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: Two newly established human esophageal squamous cell carcinoma cell lines

TitleEstablishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: Two newly established human esophageal squamous cell carcinoma cell lines
Authors
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
Citation
Cancer Genetics And Cytogenetics, 2002, v. 135 n. 2, p. 120-127 How to Cite?
AbstractThe establishment of esophageal cancer cell lines can facilitate the search for molecular mechanisms underlying its pathogenesis. Two novel human esophageal squamous cell carcinoma (ESCC) cell lines, HKESC-2 and HKESC-3, were established from a moderately differentiated ESCC of a 46-year-old Chinese woman and a well-differentiated ESCC of a 74-year-old Chinese man, both from Hong Kong. The pathological characteristics (morphological, immunohistochemical, and electron microscopic studies), tumorigenicity in nude mice, cytogenetic features, and DNA ploidy of the two cell lines were investigated. The two cell lines have been maintained in vitro for more than 17 months and passaged over 85 times for HKESC-2 and 58 times for HKESC-3. Both grew as monolayers, with a doubling time of 24 hours for HKESC-2 and 48 h for HKESC-3. Their squamous epithelial nature was authenticated by their strong immunopositivity with the anti-cytokeratin antibodies and the ultrastructural demonstration of tonofilaments and desmosomes. They are tumorigenic in nude mice and had DNA aneuploidy. G-banding cytogenetic analysis showed hyperdiploidy in HKESC-2 and near-tetraploidy in HKESC-3. Frequent breakpoints were noted at 1p22, 1p32, and 9q34 in HKESC-2 and at 1p31, 3p25, 3p14, 6q16, 6q21, 8p21, 9q34, 13q32, and 17q25 in HKESC-3. Comparative genomic hybridization analysis found that chromosomal gains were at 3q24∼qter, 5q21∼qter, 8q11∼qter, 13q21∼q31, 17q11∼qter, 19, 22q22 for HKESC-2 and at 3q13∼qter, 5p, 6p, 9q21∼qter, 10q21∼q22, 12q15∼pter, 14q24∼qter, 16, 17q24∼qter, 20 for HKESC-3. Chromosomal losses were at 3p13∼pter, 18q12∼qter for HKESC-3. These two newly established cell lines will be useful tools in the study of the molecular pathogenesis and biological behavior of ESCC cells and for testing new therapeutic reagents for ESCC in the future. © 2002 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/78452
ISSN
2012 Impact Factor: 1.929
2013 SCImago Journal Rankings: 0.872
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, YCen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorLaw, SYen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorMa, ESKen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T07:43:03Z-
dc.date.available2010-09-06T07:43:03Z-
dc.date.issued2002en_HK
dc.identifier.citationCancer Genetics And Cytogenetics, 2002, v. 135 n. 2, p. 120-127en_HK
dc.identifier.issn0165-4608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/78452-
dc.description.abstractThe establishment of esophageal cancer cell lines can facilitate the search for molecular mechanisms underlying its pathogenesis. Two novel human esophageal squamous cell carcinoma (ESCC) cell lines, HKESC-2 and HKESC-3, were established from a moderately differentiated ESCC of a 46-year-old Chinese woman and a well-differentiated ESCC of a 74-year-old Chinese man, both from Hong Kong. The pathological characteristics (morphological, immunohistochemical, and electron microscopic studies), tumorigenicity in nude mice, cytogenetic features, and DNA ploidy of the two cell lines were investigated. The two cell lines have been maintained in vitro for more than 17 months and passaged over 85 times for HKESC-2 and 58 times for HKESC-3. Both grew as monolayers, with a doubling time of 24 hours for HKESC-2 and 48 h for HKESC-3. Their squamous epithelial nature was authenticated by their strong immunopositivity with the anti-cytokeratin antibodies and the ultrastructural demonstration of tonofilaments and desmosomes. They are tumorigenic in nude mice and had DNA aneuploidy. G-banding cytogenetic analysis showed hyperdiploidy in HKESC-2 and near-tetraploidy in HKESC-3. Frequent breakpoints were noted at 1p22, 1p32, and 9q34 in HKESC-2 and at 1p31, 3p25, 3p14, 6q16, 6q21, 8p21, 9q34, 13q32, and 17q25 in HKESC-3. Comparative genomic hybridization analysis found that chromosomal gains were at 3q24∼qter, 5q21∼qter, 8q11∼qter, 13q21∼q31, 17q11∼qter, 19, 22q22 for HKESC-2 and at 3q13∼qter, 5p, 6p, 9q21∼qter, 10q21∼q22, 12q15∼pter, 14q24∼qter, 16, 17q24∼qter, 20 for HKESC-3. Chromosomal losses were at 3p13∼pter, 18q12∼qter for HKESC-3. These two newly established cell lines will be useful tools in the study of the molecular pathogenesis and biological behavior of ESCC cells and for testing new therapeutic reagents for ESCC in the future. © 2002 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergeneen_HK
dc.relation.ispartofCancer Genetics and Cytogeneticsen_HK
dc.rightsCancer Genetics and Cytogenetics. Copyright © Elsevier Inc.en_HK
dc.subject.meshAgeden_HK
dc.subject.meshAneuploidyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAsian Continental Ancestry Groupen_HK
dc.subject.meshCarcinoma, Squamous Cell - genetics - pathologyen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshChromosomes, Human - ultrastructureen_HK
dc.subject.meshEsophageal Neoplasms - genetics - pathologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshKeratins - analysisen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNeoplasm Proteins - analysisen_HK
dc.subject.meshNeoplasm Transplantationen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.subject.meshTumor Cells, Cultured - chemistry - pathologyen_HK
dc.titleEstablishment, characterization, karyotyping, and comparative genomic hybridization analysis of HKESC-2 and HKESC-3: Two newly established human esophageal squamous cell carcinoma cell linesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-4608&volume=135&spage=120&epage=127&date=2002&atitle=Establishment,+characterization,+karyotyping,+and+comparative+genomic+hybridization+analysis+of+HKESC-2+and+HKESC-3:+two+newly+established+human+esophageal+squamous+cell+carcinoma+cell+linesen_HK
dc.identifier.emailLaw, SY: slaw@hku.hken_HK
dc.identifier.emailKwong, YL: ylkwong@hku.hken_HK
dc.identifier.emailChan, LC: chanlc@hkucc.hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.authorityLaw, SY=rp00437en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0165-4608(01)00580-5en_HK
dc.identifier.pmid12127396-
dc.identifier.scopuseid_2-s2.0-0036070581en_HK
dc.identifier.hkuros67701en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036070581&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume135en_HK
dc.identifier.issue2en_HK
dc.identifier.spage120en_HK
dc.identifier.epage127en_HK
dc.identifier.isiWOS:000176967700003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, YC=16642628100en_HK
dc.identifier.scopusauthoridLam, KY=7403657165en_HK
dc.identifier.scopusauthoridLaw, SY=7202241293en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridMa, ESK=7202039934en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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